Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_389 and Pf6N2E2_390 are separated by 58 nucleotides Pf6N2E2_390 and Pf6N2E2_391 are separated by 48 nucleotides
Pf6N2E2_389 - Choline dehydrogenase (EC 1.1.99.1), at 456,997 to 458,562
_389
Pf6N2E2_390 - hypothetical protein, at 458,621 to 459,394
_390
Pf6N2E2_391 - hypothetical protein, at 459,443 to 460,921
_391
Position (kb) 458
459
460 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 457.632 kb on - strand, within Pf6N2E2_389 at 457.641 kb on + strand, within Pf6N2E2_389 at 457.808 kb on + strand, within Pf6N2E2_389 at 457.937 kb on + strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.947 kb on - strand, within Pf6N2E2_389 at 457.978 kb on + strand, within Pf6N2E2_389 at 457.979 kb on - strand, within Pf6N2E2_389 at 457.979 kb on - strand, within Pf6N2E2_389 at 458.183 kb on + strand, within Pf6N2E2_389 at 458.183 kb on + strand, within Pf6N2E2_389 at 458.183 kb on + strand, within Pf6N2E2_389 at 458.538 kb on - strand at 458.538 kb on - strand at 458.794 kb on - strand, within Pf6N2E2_390 at 458.794 kb on - strand, within Pf6N2E2_390 at 458.794 kb on - strand, within Pf6N2E2_390 at 458.943 kb on + strand, within Pf6N2E2_390 at 458.944 kb on - strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.055 kb on - strand, within Pf6N2E2_390 at 459.288 kb on - strand, within Pf6N2E2_390 at 459.288 kb on - strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.459 kb on + strand at 459.899 kb on + strand, within Pf6N2E2_391 at 459.899 kb on + strand, within Pf6N2E2_391 at 459.900 kb on - strand, within Pf6N2E2_391 at 459.900 kb on - strand, within Pf6N2E2_391 at 460.310 kb on + strand, within Pf6N2E2_391 at 460.310 kb on + strand, within Pf6N2E2_391
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 457,632 - Pf6N2E2_389 0.41 +0.7 457,641 + Pf6N2E2_389 0.41 +1.9 457,808 + Pf6N2E2_389 0.52 -0.5 457,937 + Pf6N2E2_389 0.60 +0.8 457,938 - Pf6N2E2_389 0.60 -0.9 457,938 - Pf6N2E2_389 0.60 -0.2 457,938 - Pf6N2E2_389 0.60 +0.5 457,938 - Pf6N2E2_389 0.60 +0.1 457,947 - Pf6N2E2_389 0.61 +0.3 457,978 + Pf6N2E2_389 0.63 -0.1 457,979 - Pf6N2E2_389 0.63 +0.4 457,979 - Pf6N2E2_389 0.63 +0.2 458,183 + Pf6N2E2_389 0.76 -0.9 458,183 + Pf6N2E2_389 0.76 +1.1 458,183 + Pf6N2E2_389 0.76 +0.7 458,538 - +0.7 458,538 - +0.2 458,794 - Pf6N2E2_390 0.22 -1.5 458,794 - Pf6N2E2_390 0.22 -0.7 458,794 - Pf6N2E2_390 0.22 -0.0 458,943 + Pf6N2E2_390 0.42 -0.3 458,944 - Pf6N2E2_390 0.42 +0.7 459,054 + Pf6N2E2_390 0.56 -0.0 459,054 + Pf6N2E2_390 0.56 -0.3 459,054 + Pf6N2E2_390 0.56 -0.4 459,055 - Pf6N2E2_390 0.56 -0.5 459,288 - Pf6N2E2_390 0.86 -0.3 459,288 - Pf6N2E2_390 0.86 +0.2 459,310 + Pf6N2E2_390 0.89 -2.1 459,310 + Pf6N2E2_390 0.89 -0.5 459,310 + Pf6N2E2_390 0.89 +0.2 459,311 - Pf6N2E2_390 0.89 -0.3 459,311 - Pf6N2E2_390 0.89 -0.5 459,311 - Pf6N2E2_390 0.89 -0.3 459,311 - Pf6N2E2_390 0.89 +0.8 459,311 - Pf6N2E2_390 0.89 -0.8 459,459 + +0.6 459,899 + Pf6N2E2_391 0.31 -0.5 459,899 + Pf6N2E2_391 0.31 +0.3 459,900 - Pf6N2E2_391 0.31 -0.8 459,900 - Pf6N2E2_391 0.31 -2.9 460,310 + Pf6N2E2_391 0.59 -0.6 460,310 + Pf6N2E2_391 0.59 +0.4
Or see this region's nucleotide sequence