Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6000 and Pf6N2E2_6001 are separated by 89 nucleotides Pf6N2E2_6001 and Pf6N2E2_6002 are separated by 131 nucleotides
Pf6N2E2_6000 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 6,816,772 to 6,818,916
_6000
Pf6N2E2_6001 - Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85), at 6,819,006 to 6,819,740
_6001
Pf6N2E2_6002 - Magnesium and cobalt transport protein CorA, at 6,819,872 to 6,820,843
_6002
Position (kb)
6819
6820 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6818.298 kb on + strand, within Pf6N2E2_6000 at 6818.299 kb on - strand, within Pf6N2E2_6000 at 6818.299 kb on - strand, within Pf6N2E2_6000 at 6818.525 kb on - strand, within Pf6N2E2_6000 at 6818.525 kb on - strand, within Pf6N2E2_6000 at 6818.560 kb on + strand, within Pf6N2E2_6000 at 6818.560 kb on + strand, within Pf6N2E2_6000 at 6818.560 kb on + strand, within Pf6N2E2_6000 at 6818.561 kb on - strand, within Pf6N2E2_6000 at 6818.587 kb on + strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.588 kb on - strand, within Pf6N2E2_6000 at 6818.699 kb on + strand, within Pf6N2E2_6000 at 6818.836 kb on + strand at 6818.978 kb on - strand at 6818.998 kb on + strand at 6819.005 kb on + strand at 6819.005 kb on + strand at 6819.006 kb on - strand at 6819.006 kb on - strand at 6819.013 kb on + strand at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.135 kb on + strand, within Pf6N2E2_6001 at 6819.136 kb on - strand, within Pf6N2E2_6001 at 6819.136 kb on - strand, within Pf6N2E2_6001 at 6819.186 kb on + strand, within Pf6N2E2_6001 at 6819.186 kb on + strand, within Pf6N2E2_6001 at 6819.252 kb on + strand, within Pf6N2E2_6001 at 6819.252 kb on + strand, within Pf6N2E2_6001 at 6819.252 kb on + strand, within Pf6N2E2_6001 at 6819.253 kb on - strand, within Pf6N2E2_6001 at 6819.523 kb on - strand, within Pf6N2E2_6001 at 6819.580 kb on - strand, within Pf6N2E2_6001 at 6819.580 kb on - strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.630 kb on + strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.631 kb on - strand, within Pf6N2E2_6001 at 6819.633 kb on - strand, within Pf6N2E2_6001 at 6819.675 kb on - strand at 6819.675 kb on - strand at 6819.873 kb on + strand at 6819.873 kb on + strand at 6819.874 kb on - strand at 6819.928 kb on + strand at 6819.929 kb on - strand at 6819.929 kb on - strand at 6819.935 kb on + strand at 6819.935 kb on + strand at 6819.935 kb on + strand at 6819.936 kb on - strand at 6819.936 kb on - strand at 6819.936 kb on - strand at 6819.936 kb on - strand at 6819.936 kb on - strand at 6819.936 kb on - strand at 6819.954 kb on + strand at 6819.996 kb on + strand, within Pf6N2E2_6002 at 6819.996 kb on + strand, within Pf6N2E2_6002 at 6819.997 kb on - strand, within Pf6N2E2_6002 at 6819.997 kb on - strand, within Pf6N2E2_6002 at 6820.180 kb on - strand, within Pf6N2E2_6002 at 6820.180 kb on - strand, within Pf6N2E2_6002 at 6820.180 kb on - strand, within Pf6N2E2_6002 at 6820.274 kb on + strand, within Pf6N2E2_6002 at 6820.389 kb on + strand, within Pf6N2E2_6002 at 6820.389 kb on + strand, within Pf6N2E2_6002 at 6820.389 kb on + strand, within Pf6N2E2_6002 at 6820.390 kb on - strand, within Pf6N2E2_6002 at 6820.390 kb on - strand, within Pf6N2E2_6002 at 6820.524 kb on + strand, within Pf6N2E2_6002 at 6820.525 kb on - strand, within Pf6N2E2_6002 at 6820.525 kb on - strand, within Pf6N2E2_6002 at 6820.525 kb on - strand, within Pf6N2E2_6002 at 6820.544 kb on - strand, within Pf6N2E2_6002 at 6820.590 kb on + strand, within Pf6N2E2_6002 at 6820.591 kb on - strand, within Pf6N2E2_6002 at 6820.600 kb on + strand, within Pf6N2E2_6002 at 6820.740 kb on - strand, within Pf6N2E2_6002
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 6,818,298 + Pf6N2E2_6000 0.71 +0.9 6,818,299 - Pf6N2E2_6000 0.71 +0.4 6,818,299 - Pf6N2E2_6000 0.71 +0.2 6,818,525 - Pf6N2E2_6000 0.82 -0.3 6,818,525 - Pf6N2E2_6000 0.82 -0.1 6,818,560 + Pf6N2E2_6000 0.83 -1.0 6,818,560 + Pf6N2E2_6000 0.83 -0.6 6,818,560 + Pf6N2E2_6000 0.83 -0.4 6,818,561 - Pf6N2E2_6000 0.83 +0.6 6,818,587 + Pf6N2E2_6000 0.85 +0.6 6,818,588 - Pf6N2E2_6000 0.85 +0.7 6,818,588 - Pf6N2E2_6000 0.85 +0.7 6,818,588 - Pf6N2E2_6000 0.85 -0.7 6,818,588 - Pf6N2E2_6000 0.85 +0.4 6,818,699 + Pf6N2E2_6000 0.90 +0.2 6,818,836 + +0.4 6,818,978 - -0.4 6,818,998 + +0.4 6,819,005 + +0.3 6,819,005 + -0.8 6,819,006 - -0.4 6,819,006 - -0.5 6,819,013 + +0.0 6,819,135 + Pf6N2E2_6001 0.18 +0.6 6,819,135 + Pf6N2E2_6001 0.18 +0.2 6,819,135 + Pf6N2E2_6001 0.18 -0.6 6,819,135 + Pf6N2E2_6001 0.18 +0.4 6,819,136 - Pf6N2E2_6001 0.18 +0.2 6,819,136 - Pf6N2E2_6001 0.18 +0.5 6,819,186 + Pf6N2E2_6001 0.24 -0.5 6,819,186 + Pf6N2E2_6001 0.24 +0.2 6,819,252 + Pf6N2E2_6001 0.33 +0.1 6,819,252 + Pf6N2E2_6001 0.33 -0.1 6,819,252 + Pf6N2E2_6001 0.33 +0.1 6,819,253 - Pf6N2E2_6001 0.34 +1.5 6,819,523 - Pf6N2E2_6001 0.70 +0.1 6,819,580 - Pf6N2E2_6001 0.78 +0.3 6,819,580 - Pf6N2E2_6001 0.78 -0.3 6,819,630 + Pf6N2E2_6001 0.85 +0.1 6,819,630 + Pf6N2E2_6001 0.85 +0.4 6,819,630 + Pf6N2E2_6001 0.85 +0.2 6,819,630 + Pf6N2E2_6001 0.85 +0.5 6,819,630 + Pf6N2E2_6001 0.85 +0.3 6,819,630 + Pf6N2E2_6001 0.85 +0.0 6,819,630 + Pf6N2E2_6001 0.85 +0.6 6,819,630 + Pf6N2E2_6001 0.85 +0.5 6,819,631 - Pf6N2E2_6001 0.85 +0.8 6,819,631 - Pf6N2E2_6001 0.85 +0.4 6,819,631 - Pf6N2E2_6001 0.85 +2.1 6,819,631 - Pf6N2E2_6001 0.85 -0.8 6,819,633 - Pf6N2E2_6001 0.85 +0.8 6,819,675 - +0.5 6,819,675 - +0.7 6,819,873 + -1.9 6,819,873 + +0.6 6,819,874 - +0.6 6,819,928 + +0.2 6,819,929 - -0.0 6,819,929 - +0.6 6,819,935 + -1.1 6,819,935 + +0.3 6,819,935 + +0.9 6,819,936 - -0.9 6,819,936 - -0.1 6,819,936 - -0.6 6,819,936 - -2.6 6,819,936 - +0.9 6,819,936 - -0.9 6,819,954 + -1.5 6,819,996 + Pf6N2E2_6002 0.13 +0.1 6,819,996 + Pf6N2E2_6002 0.13 -0.9 6,819,997 - Pf6N2E2_6002 0.13 -0.5 6,819,997 - Pf6N2E2_6002 0.13 -0.7 6,820,180 - Pf6N2E2_6002 0.32 -1.7 6,820,180 - Pf6N2E2_6002 0.32 +0.4 6,820,180 - Pf6N2E2_6002 0.32 -0.4 6,820,274 + Pf6N2E2_6002 0.41 -0.6 6,820,389 + Pf6N2E2_6002 0.53 +1.2 6,820,389 + Pf6N2E2_6002 0.53 +0.1 6,820,389 + Pf6N2E2_6002 0.53 -0.1 6,820,390 - Pf6N2E2_6002 0.53 +0.5 6,820,390 - Pf6N2E2_6002 0.53 +0.2 6,820,524 + Pf6N2E2_6002 0.67 +1.9 6,820,525 - Pf6N2E2_6002 0.67 -2.3 6,820,525 - Pf6N2E2_6002 0.67 +0.1 6,820,525 - Pf6N2E2_6002 0.67 -0.5 6,820,544 - Pf6N2E2_6002 0.69 +0.9 6,820,590 + Pf6N2E2_6002 0.74 -0.2 6,820,591 - Pf6N2E2_6002 0.74 +1.1 6,820,600 + Pf6N2E2_6002 0.75 +1.2 6,820,740 - Pf6N2E2_6002 0.89 -0.7
Or see this region's nucleotide sequence