Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5544 and Pf6N2E2_5545 are separated by 166 nucleotides Pf6N2E2_5545 and Pf6N2E2_5546 are separated by 1 nucleotides Pf6N2E2_5546 and Pf6N2E2_6157 are separated by 63 nucleotides Pf6N2E2_6157 and Pf6N2E2_5547 are separated by 152 nucleotides
Pf6N2E2_5544 - TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain, at 6,358,942 to 6,359,706
_5544
Pf6N2E2_5545 - Queuosine Biosynthesis QueE Radical SAM, at 6,359,873 to 6,360,520
_5545
Pf6N2E2_5546 - Queuosine Biosynthesis QueC ATPase, at 6,360,522 to 6,361,214
_5546
Pf6N2E2_6157 - tRNA-Lys-TTT, at 6,361,278 to 6,361,350
_6157
Pf6N2E2_5547 - hypothetical protein, at 6,361,503 to 6,362,933
_5547
Position (kb)
6359
6360
6361 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 6358.889 kb on + strand at 6358.965 kb on + strand at 6358.965 kb on + strand at 6358.980 kb on + strand at 6359.192 kb on + strand, within Pf6N2E2_5544 at 6359.193 kb on - strand, within Pf6N2E2_5544 at 6359.350 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.354 kb on - strand, within Pf6N2E2_5544 at 6359.431 kb on + strand, within Pf6N2E2_5544 at 6359.431 kb on + strand, within Pf6N2E2_5544 at 6359.431 kb on + strand, within Pf6N2E2_5544 at 6359.464 kb on + strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.575 kb on + strand, within Pf6N2E2_5544 at 6359.591 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.705 kb on - strand at 6359.705 kb on - strand at 6359.777 kb on - strand at 6359.850 kb on + strand at 6359.857 kb on + strand at 6359.858 kb on - strand at 6359.873 kb on - strand at 6359.949 kb on + strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.987 kb on + strand, within Pf6N2E2_5545 at 6360.063 kb on - strand, within Pf6N2E2_5545 at 6360.074 kb on + strand, within Pf6N2E2_5545 at 6360.075 kb on - strand, within Pf6N2E2_5545 at 6360.188 kb on - strand, within Pf6N2E2_5545 at 6360.362 kb on + strand, within Pf6N2E2_5545 at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.488 kb on - strand at 6360.488 kb on - strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.492 kb on - strand at 6360.521 kb on + strand at 6360.521 kb on + strand at 6360.522 kb on - strand at 6360.522 kb on - strand at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.649 kb on - strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.833 kb on + strand, within Pf6N2E2_5546 at 6360.833 kb on + strand, within Pf6N2E2_5546 at 6360.841 kb on + strand, within Pf6N2E2_5546 at 6360.842 kb on - strand, within Pf6N2E2_5546 at 6361.424 kb on + strand at 6361.424 kb on + strand at 6361.424 kb on + strand at 6361.425 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 6,358,889 + +0.8 6,358,965 + -0.9 6,358,965 + -0.9 6,358,980 + -2.5 6,359,192 + Pf6N2E2_5544 0.33 -0.8 6,359,193 - Pf6N2E2_5544 0.33 +0.1 6,359,350 + Pf6N2E2_5544 0.53 -0.4 6,359,353 + Pf6N2E2_5544 0.54 -0.5 6,359,353 + Pf6N2E2_5544 0.54 -1.4 6,359,353 + Pf6N2E2_5544 0.54 +0.8 6,359,353 + Pf6N2E2_5544 0.54 -0.7 6,359,353 + Pf6N2E2_5544 0.54 +0.3 6,359,354 - Pf6N2E2_5544 0.54 -0.1 6,359,431 + Pf6N2E2_5544 0.64 +0.7 6,359,431 + Pf6N2E2_5544 0.64 -0.2 6,359,431 + Pf6N2E2_5544 0.64 -0.2 6,359,464 + Pf6N2E2_5544 0.68 +0.2 6,359,543 - Pf6N2E2_5544 0.79 -0.2 6,359,543 - Pf6N2E2_5544 0.79 -1.0 6,359,543 - Pf6N2E2_5544 0.79 +0.6 6,359,543 - Pf6N2E2_5544 0.79 -1.2 6,359,575 + Pf6N2E2_5544 0.83 +0.3 6,359,591 + Pf6N2E2_5544 0.85 -0.5 6,359,621 + Pf6N2E2_5544 0.89 -1.1 6,359,621 + Pf6N2E2_5544 0.89 -0.6 6,359,621 + Pf6N2E2_5544 0.89 +0.3 6,359,621 + Pf6N2E2_5544 0.89 +0.4 6,359,622 - Pf6N2E2_5544 0.89 +0.2 6,359,622 - Pf6N2E2_5544 0.89 -0.4 6,359,622 - Pf6N2E2_5544 0.89 +1.2 6,359,704 + -0.2 6,359,704 + +0.4 6,359,704 + +0.6 6,359,704 + +1.1 6,359,705 - +0.6 6,359,705 - -0.2 6,359,777 - -1.0 6,359,850 + +0.3 6,359,857 + -0.3 6,359,858 - +0.3 6,359,873 - -0.3 6,359,949 + Pf6N2E2_5545 0.12 -0.8 6,359,950 - Pf6N2E2_5545 0.12 -1.1 6,359,950 - Pf6N2E2_5545 0.12 -0.6 6,359,987 + Pf6N2E2_5545 0.18 -0.7 6,360,063 - Pf6N2E2_5545 0.29 -0.1 6,360,074 + Pf6N2E2_5545 0.31 -0.1 6,360,075 - Pf6N2E2_5545 0.31 -0.7 6,360,188 - Pf6N2E2_5545 0.49 -0.6 6,360,362 + Pf6N2E2_5545 0.75 -0.9 6,360,487 + -0.1 6,360,487 + -0.3 6,360,487 + +2.3 6,360,488 - +0.4 6,360,488 - +0.2 6,360,491 + -0.9 6,360,491 + -3.0 6,360,491 + -0.6 6,360,492 - +0.1 6,360,521 + +1.7 6,360,521 + -0.2 6,360,522 - +0.7 6,360,522 - +1.1 6,360,631 - Pf6N2E2_5546 0.16 -0.6 6,360,631 - Pf6N2E2_5546 0.16 +1.1 6,360,631 - Pf6N2E2_5546 0.16 -0.5 6,360,649 - Pf6N2E2_5546 0.18 -1.6 6,360,819 + Pf6N2E2_5546 0.43 -0.5 6,360,819 + Pf6N2E2_5546 0.43 +1.1 6,360,819 + Pf6N2E2_5546 0.43 -1.9 6,360,819 + Pf6N2E2_5546 0.43 +0.8 6,360,819 + Pf6N2E2_5546 0.43 -0.4 6,360,819 + Pf6N2E2_5546 0.43 +3.9 6,360,819 + Pf6N2E2_5546 0.43 -0.3 6,360,819 + Pf6N2E2_5546 0.43 -0.9 6,360,819 + Pf6N2E2_5546 0.43 -0.3 6,360,819 + Pf6N2E2_5546 0.43 +0.6 6,360,819 + Pf6N2E2_5546 0.43 -0.2 6,360,820 - Pf6N2E2_5546 0.43 -0.6 6,360,820 - Pf6N2E2_5546 0.43 +0.6 6,360,820 - Pf6N2E2_5546 0.43 +0.7 6,360,820 - Pf6N2E2_5546 0.43 +0.4 6,360,820 - Pf6N2E2_5546 0.43 -2.7 6,360,820 - Pf6N2E2_5546 0.43 +0.1 6,360,833 + Pf6N2E2_5546 0.45 +0.1 6,360,833 + Pf6N2E2_5546 0.45 -1.1 6,360,841 + Pf6N2E2_5546 0.46 +0.1 6,360,842 - Pf6N2E2_5546 0.46 +0.7 6,361,424 + -0.6 6,361,424 + +0.1 6,361,424 + -0.2 6,361,425 - -1.5
Or see this region's nucleotide sequence