Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5503 and Pf6N2E2_5504 overlap by 4 nucleotides Pf6N2E2_5504 and Pf6N2E2_5505 are separated by 12 nucleotides Pf6N2E2_5505 and Pf6N2E2_5506 are separated by 119 nucleotides Pf6N2E2_5506 and Pf6N2E2_5507 are separated by 81 nucleotides Pf6N2E2_5507 and Pf6N2E2_5508 are separated by 306 nucleotides
Pf6N2E2_5503 - FIG01248689: hypothetical protein, at 6,321,480 to 6,322,454
_5503
Pf6N2E2_5504 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 6,322,451 to 6,323,176
_5504
Pf6N2E2_5505 - hypothetical protein, at 6,323,189 to 6,323,314
_5505
Pf6N2E2_5506 - Exodeoxyribonuclease I (EC 3.1.11.1), at 6,323,434 to 6,324,870
_5506
Pf6N2E2_5507 - transcriptional regulator MvaT, P16 subunit, at 6,324,952 to 6,325,329
_5507
Pf6N2E2_5508 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 6,325,636 to 6,326,484
_5508
Position (kb)
6323
6324
6325 Strain fitness (log2 ratio)
-1
0
1 at 6322.923 kb on + strand, within Pf6N2E2_5504 at 6322.923 kb on + strand, within Pf6N2E2_5504 at 6322.923 kb on + strand, within Pf6N2E2_5504 at 6322.923 kb on + strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6323.025 kb on - strand, within Pf6N2E2_5504 at 6323.226 kb on + strand, within Pf6N2E2_5505 at 6323.226 kb on + strand, within Pf6N2E2_5505 at 6323.251 kb on + strand, within Pf6N2E2_5505 at 6323.310 kb on + strand at 6323.310 kb on + strand at 6323.310 kb on + strand at 6323.311 kb on - strand at 6323.311 kb on - strand at 6323.401 kb on - strand at 6323.401 kb on - strand at 6323.458 kb on + strand at 6323.459 kb on - strand at 6323.560 kb on + strand at 6323.560 kb on + strand at 6323.845 kb on + strand, within Pf6N2E2_5506 at 6323.895 kb on - strand, within Pf6N2E2_5506 at 6324.096 kb on + strand, within Pf6N2E2_5506 at 6324.096 kb on + strand, within Pf6N2E2_5506 at 6324.097 kb on - strand, within Pf6N2E2_5506 at 6324.127 kb on + strand, within Pf6N2E2_5506 at 6324.128 kb on - strand, within Pf6N2E2_5506 at 6324.128 kb on - strand, within Pf6N2E2_5506 at 6324.186 kb on - strand, within Pf6N2E2_5506 at 6324.186 kb on - strand, within Pf6N2E2_5506 at 6324.253 kb on + strand, within Pf6N2E2_5506 at 6324.253 kb on + strand, within Pf6N2E2_5506 at 6324.253 kb on + strand, within Pf6N2E2_5506 at 6324.253 kb on + strand, within Pf6N2E2_5506 at 6324.254 kb on - strand, within Pf6N2E2_5506 at 6324.254 kb on - strand, within Pf6N2E2_5506 at 6324.254 kb on - strand, within Pf6N2E2_5506 at 6324.254 kb on - strand, within Pf6N2E2_5506 at 6324.254 kb on - strand, within Pf6N2E2_5506 at 6324.254 kb on - strand, within Pf6N2E2_5506 at 6324.256 kb on - strand, within Pf6N2E2_5506 at 6324.307 kb on - strand, within Pf6N2E2_5506 at 6324.394 kb on + strand, within Pf6N2E2_5506 at 6324.395 kb on - strand, within Pf6N2E2_5506 at 6324.456 kb on + strand, within Pf6N2E2_5506 at 6324.456 kb on + strand, within Pf6N2E2_5506 at 6324.456 kb on + strand, within Pf6N2E2_5506 at 6324.457 kb on - strand, within Pf6N2E2_5506 at 6324.457 kb on - strand, within Pf6N2E2_5506 at 6324.460 kb on + strand, within Pf6N2E2_5506 at 6324.506 kb on + strand, within Pf6N2E2_5506 at 6324.506 kb on + strand, within Pf6N2E2_5506 at 6324.507 kb on - strand, within Pf6N2E2_5506 at 6324.507 kb on - strand, within Pf6N2E2_5506 at 6324.507 kb on - strand, within Pf6N2E2_5506 at 6324.508 kb on + strand, within Pf6N2E2_5506 at 6324.542 kb on + strand, within Pf6N2E2_5506 at 6324.542 kb on + strand, within Pf6N2E2_5506 at 6324.543 kb on - strand, within Pf6N2E2_5506 at 6324.543 kb on - strand, within Pf6N2E2_5506 at 6324.624 kb on - strand, within Pf6N2E2_5506 at 6324.624 kb on - strand, within Pf6N2E2_5506 at 6324.624 kb on - strand, within Pf6N2E2_5506 at 6324.738 kb on + strand at 6324.738 kb on + strand at 6324.738 kb on + strand at 6324.739 kb on - strand at 6324.786 kb on + strand at 6324.807 kb on - strand at 6325.379 kb on - strand at 6325.382 kb on - strand at 6325.503 kb on + strand at 6325.503 kb on + strand at 6325.505 kb on + strand at 6325.505 kb on + strand at 6325.505 kb on + strand at 6325.506 kb on - strand at 6325.506 kb on - strand at 6325.506 kb on - strand at 6325.598 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 6,322,923 + Pf6N2E2_5504 0.65 +0.5 6,322,923 + Pf6N2E2_5504 0.65 -0.5 6,322,923 + Pf6N2E2_5504 0.65 -0.1 6,322,923 + Pf6N2E2_5504 0.65 -0.2 6,322,924 - Pf6N2E2_5504 0.65 +0.5 6,322,924 - Pf6N2E2_5504 0.65 +1.6 6,322,924 - Pf6N2E2_5504 0.65 +0.8 6,322,924 - Pf6N2E2_5504 0.65 +0.4 6,322,924 - Pf6N2E2_5504 0.65 +1.0 6,322,924 - Pf6N2E2_5504 0.65 -0.2 6,323,025 - Pf6N2E2_5504 0.79 +0.4 6,323,226 + Pf6N2E2_5505 0.29 +0.2 6,323,226 + Pf6N2E2_5505 0.29 +0.3 6,323,251 + Pf6N2E2_5505 0.49 -1.4 6,323,310 + -0.2 6,323,310 + -0.8 6,323,310 + +1.1 6,323,311 - -0.2 6,323,311 - -0.6 6,323,401 - -0.3 6,323,401 - +1.1 6,323,458 + -1.2 6,323,459 - -0.3 6,323,560 + +0.1 6,323,560 + +0.1 6,323,845 + Pf6N2E2_5506 0.29 -1.5 6,323,895 - Pf6N2E2_5506 0.32 +0.9 6,324,096 + Pf6N2E2_5506 0.46 -0.6 6,324,096 + Pf6N2E2_5506 0.46 +0.7 6,324,097 - Pf6N2E2_5506 0.46 +0.3 6,324,127 + Pf6N2E2_5506 0.48 -0.1 6,324,128 - Pf6N2E2_5506 0.48 -0.6 6,324,128 - Pf6N2E2_5506 0.48 +1.5 6,324,186 - Pf6N2E2_5506 0.52 -0.6 6,324,186 - Pf6N2E2_5506 0.52 -0.3 6,324,253 + Pf6N2E2_5506 0.57 +0.1 6,324,253 + Pf6N2E2_5506 0.57 -0.4 6,324,253 + Pf6N2E2_5506 0.57 -0.4 6,324,253 + Pf6N2E2_5506 0.57 -0.4 6,324,254 - Pf6N2E2_5506 0.57 -0.1 6,324,254 - Pf6N2E2_5506 0.57 -0.9 6,324,254 - Pf6N2E2_5506 0.57 +0.2 6,324,254 - Pf6N2E2_5506 0.57 +0.9 6,324,254 - Pf6N2E2_5506 0.57 -1.5 6,324,254 - Pf6N2E2_5506 0.57 +0.1 6,324,256 - Pf6N2E2_5506 0.57 +0.6 6,324,307 - Pf6N2E2_5506 0.61 +0.4 6,324,394 + Pf6N2E2_5506 0.67 -0.5 6,324,395 - Pf6N2E2_5506 0.67 -0.9 6,324,456 + Pf6N2E2_5506 0.71 -0.6 6,324,456 + Pf6N2E2_5506 0.71 -0.7 6,324,456 + Pf6N2E2_5506 0.71 +1.1 6,324,457 - Pf6N2E2_5506 0.71 +0.9 6,324,457 - Pf6N2E2_5506 0.71 -0.2 6,324,460 + Pf6N2E2_5506 0.71 -0.2 6,324,506 + Pf6N2E2_5506 0.75 +0.5 6,324,506 + Pf6N2E2_5506 0.75 -0.8 6,324,507 - Pf6N2E2_5506 0.75 -0.9 6,324,507 - Pf6N2E2_5506 0.75 +0.7 6,324,507 - Pf6N2E2_5506 0.75 +0.3 6,324,508 + Pf6N2E2_5506 0.75 +0.1 6,324,542 + Pf6N2E2_5506 0.77 -0.2 6,324,542 + Pf6N2E2_5506 0.77 +1.0 6,324,543 - Pf6N2E2_5506 0.77 +0.8 6,324,543 - Pf6N2E2_5506 0.77 -0.2 6,324,624 - Pf6N2E2_5506 0.83 +0.5 6,324,624 - Pf6N2E2_5506 0.83 +0.9 6,324,624 - Pf6N2E2_5506 0.83 +0.2 6,324,738 + +0.5 6,324,738 + -0.0 6,324,738 + -1.4 6,324,739 - +0.2 6,324,786 + -0.4 6,324,807 - -0.5 6,325,379 - +1.2 6,325,382 - +0.5 6,325,503 + +1.7 6,325,503 + +1.4 6,325,505 + -0.1 6,325,505 + +0.4 6,325,505 + -1.4 6,325,506 - +0.1 6,325,506 - -1.1 6,325,506 - +0.2 6,325,598 + +0.5
Or see this region's nucleotide sequence