Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4537 and Pf6N2E2_4538 overlap by 1 nucleotides Pf6N2E2_4538 and Pf6N2E2_4539 are separated by 15 nucleotides Pf6N2E2_4539 and Pf6N2E2_4540 are separated by 419 nucleotides Pf6N2E2_4540 and Pf6N2E2_4541 are separated by 124 nucleotides
Pf6N2E2_4537 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 5,271,079 to 5,271,333
_4537
Pf6N2E2_4538 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 5,271,333 to 5,272,130
_4538
Pf6N2E2_4539 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 5,272,146 to 5,273,246
_4539
Pf6N2E2_4540 - hypothetical protein, at 5,273,666 to 5,273,881
_4540
Pf6N2E2_4541 - hypothetical protein, at 5,274,006 to 5,274,455
_4541
Position (kb) 5272
5273
5274 Strain fitness (log2 ratio) -1
0
1 at 5271.167 kb on + strand, within Pf6N2E2_4537 at 5271.167 kb on + strand, within Pf6N2E2_4537 at 5271.328 kb on - strand at 5271.357 kb on + strand at 5271.403 kb on - strand at 5271.414 kb on + strand, within Pf6N2E2_4538 at 5271.621 kb on + strand, within Pf6N2E2_4538 at 5271.621 kb on + strand, within Pf6N2E2_4538 at 5271.621 kb on + strand, within Pf6N2E2_4538 at 5271.622 kb on - strand, within Pf6N2E2_4538 at 5271.622 kb on - strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.964 kb on - strand, within Pf6N2E2_4538 at 5271.964 kb on - strand, within Pf6N2E2_4538 at 5271.964 kb on - strand, within Pf6N2E2_4538 at 5271.965 kb on + strand, within Pf6N2E2_4538 at 5272.039 kb on - strand, within Pf6N2E2_4538 at 5272.427 kb on - strand, within Pf6N2E2_4539 at 5272.465 kb on + strand, within Pf6N2E2_4539 at 5272.618 kb on - strand, within Pf6N2E2_4539 at 5272.680 kb on + strand, within Pf6N2E2_4539 at 5272.680 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.776 kb on + strand, within Pf6N2E2_4539 at 5272.776 kb on + strand, within Pf6N2E2_4539 at 5272.855 kb on + strand, within Pf6N2E2_4539 at 5272.855 kb on + strand, within Pf6N2E2_4539 at 5272.980 kb on + strand, within Pf6N2E2_4539 at 5272.980 kb on + strand, within Pf6N2E2_4539 at 5272.981 kb on - strand, within Pf6N2E2_4539 at 5273.113 kb on - strand, within Pf6N2E2_4539 at 5273.133 kb on + strand, within Pf6N2E2_4539 at 5273.173 kb on - strand at 5273.266 kb on + strand at 5273.394 kb on + strand at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.762 kb on - strand, within Pf6N2E2_4540 at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.898 kb on - strand at 5273.999 kb on + strand at 5273.999 kb on + strand at 5274.000 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 5,271,167 + Pf6N2E2_4537 0.35 -1.2 5,271,167 + Pf6N2E2_4537 0.35 +0.1 5,271,328 - +0.9 5,271,357 + -0.9 5,271,403 - -1.1 5,271,414 + Pf6N2E2_4538 0.10 +0.5 5,271,621 + Pf6N2E2_4538 0.36 +0.0 5,271,621 + Pf6N2E2_4538 0.36 +0.4 5,271,621 + Pf6N2E2_4538 0.36 +0.4 5,271,622 - Pf6N2E2_4538 0.36 +0.0 5,271,622 - Pf6N2E2_4538 0.36 +0.8 5,271,798 + Pf6N2E2_4538 0.58 -0.0 5,271,798 + Pf6N2E2_4538 0.58 +1.5 5,271,798 + Pf6N2E2_4538 0.58 -1.4 5,271,798 + Pf6N2E2_4538 0.58 +0.5 5,271,798 + Pf6N2E2_4538 0.58 +0.2 5,271,964 - Pf6N2E2_4538 0.79 -0.1 5,271,964 - Pf6N2E2_4538 0.79 -0.0 5,271,964 - Pf6N2E2_4538 0.79 +0.4 5,271,965 + Pf6N2E2_4538 0.79 -1.1 5,272,039 - Pf6N2E2_4538 0.88 +0.9 5,272,427 - Pf6N2E2_4539 0.26 -0.5 5,272,465 + Pf6N2E2_4539 0.29 -1.3 5,272,618 - Pf6N2E2_4539 0.43 -0.5 5,272,680 + Pf6N2E2_4539 0.49 +0.8 5,272,680 + Pf6N2E2_4539 0.49 +0.1 5,272,735 + Pf6N2E2_4539 0.53 +0.1 5,272,735 + Pf6N2E2_4539 0.53 -0.3 5,272,735 + Pf6N2E2_4539 0.53 +0.7 5,272,735 + Pf6N2E2_4539 0.53 -0.4 5,272,735 + Pf6N2E2_4539 0.53 -0.7 5,272,735 + Pf6N2E2_4539 0.53 +0.7 5,272,736 - Pf6N2E2_4539 0.54 -1.1 5,272,736 - Pf6N2E2_4539 0.54 -1.5 5,272,736 - Pf6N2E2_4539 0.54 -0.7 5,272,736 - Pf6N2E2_4539 0.54 +0.9 5,272,776 + Pf6N2E2_4539 0.57 +1.1 5,272,776 + Pf6N2E2_4539 0.57 -1.5 5,272,855 + Pf6N2E2_4539 0.64 -0.4 5,272,855 + Pf6N2E2_4539 0.64 -0.6 5,272,980 + Pf6N2E2_4539 0.76 +0.3 5,272,980 + Pf6N2E2_4539 0.76 -0.5 5,272,981 - Pf6N2E2_4539 0.76 -0.9 5,273,113 - Pf6N2E2_4539 0.88 -0.2 5,273,133 + Pf6N2E2_4539 0.90 -1.0 5,273,173 - +0.5 5,273,266 + +0.2 5,273,394 + -0.4 5,273,761 + Pf6N2E2_4540 0.44 +1.5 5,273,761 + Pf6N2E2_4540 0.44 +0.7 5,273,761 + Pf6N2E2_4540 0.44 -0.3 5,273,762 - Pf6N2E2_4540 0.44 +0.5 5,273,897 + +0.0 5,273,897 + -1.2 5,273,897 + +0.2 5,273,898 - +1.2 5,273,999 + -0.5 5,273,999 + -0.0 5,274,000 - +0.5
Or see this region's nucleotide sequence