Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2060 and Pf6N2E2_2061 are separated by 3 nucleotides Pf6N2E2_2061 and Pf6N2E2_2062 are separated by 834 nucleotides
Pf6N2E2_2060 - CobN component of cobalt chelatase involved in B12 biosynthesis, at 2,449,483 to 2,453,256
_2060
Pf6N2E2_2061 - CobW GTPase involved in cobalt insertion for B12 biosynthesis, at 2,453,260 to 2,454,324
_2061
Pf6N2E2_2062 - FIG00955561: hypothetical protein, at 2,455,159 to 2,455,347
_2062
Position (kb)
2453
2454
2455 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2452.464 kb on + strand, within Pf6N2E2_2060 at 2452.638 kb on - strand, within Pf6N2E2_2060 at 2452.721 kb on + strand, within Pf6N2E2_2060 at 2452.721 kb on + strand, within Pf6N2E2_2060 at 2452.721 kb on + strand, within Pf6N2E2_2060 at 2452.722 kb on - strand, within Pf6N2E2_2060 at 2452.722 kb on - strand, within Pf6N2E2_2060 at 2452.764 kb on - strand, within Pf6N2E2_2060 at 2453.046 kb on - strand at 2453.046 kb on - strand at 2453.261 kb on + strand at 2453.459 kb on - strand, within Pf6N2E2_2061 at 2453.648 kb on - strand, within Pf6N2E2_2061 at 2454.021 kb on + strand, within Pf6N2E2_2061 at 2454.022 kb on - strand, within Pf6N2E2_2061 at 2454.022 kb on - strand, within Pf6N2E2_2061 at 2454.116 kb on + strand, within Pf6N2E2_2061 at 2454.116 kb on + strand, within Pf6N2E2_2061 at 2454.116 kb on + strand, within Pf6N2E2_2061 at 2454.117 kb on - strand, within Pf6N2E2_2061 at 2454.422 kb on - strand at 2454.422 kb on - strand at 2454.422 kb on - strand at 2454.422 kb on - strand at 2454.510 kb on + strand at 2454.511 kb on - strand at 2454.595 kb on + strand at 2454.595 kb on + strand at 2454.596 kb on - strand at 2454.596 kb on - strand at 2454.635 kb on + strand at 2454.636 kb on - strand at 2454.670 kb on + strand at 2454.670 kb on + strand at 2454.670 kb on + strand at 2454.671 kb on - strand at 2454.671 kb on - strand at 2454.671 kb on - strand at 2454.671 kb on - strand at 2454.671 kb on - strand at 2454.671 kb on - strand at 2454.739 kb on + strand at 2454.739 kb on + strand at 2454.805 kb on + strand at 2454.805 kb on + strand at 2454.805 kb on + strand at 2454.805 kb on + strand at 2454.805 kb on + strand at 2454.805 kb on + strand at 2454.806 kb on - strand at 2454.806 kb on - strand at 2454.806 kb on - strand at 2454.806 kb on - strand at 2454.846 kb on + strand at 2454.846 kb on + strand at 2454.851 kb on - strand at 2454.900 kb on + strand at 2454.901 kb on - strand at 2455.194 kb on + strand, within Pf6N2E2_2062 at 2455.195 kb on - strand, within Pf6N2E2_2062
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 2,452,464 + Pf6N2E2_2060 0.79 +1.0 2,452,638 - Pf6N2E2_2060 0.84 +2.5 2,452,721 + Pf6N2E2_2060 0.86 -0.4 2,452,721 + Pf6N2E2_2060 0.86 -0.8 2,452,721 + Pf6N2E2_2060 0.86 +0.1 2,452,722 - Pf6N2E2_2060 0.86 -1.2 2,452,722 - Pf6N2E2_2060 0.86 +0.4 2,452,764 - Pf6N2E2_2060 0.87 -1.5 2,453,046 - -1.3 2,453,046 - -1.6 2,453,261 + +0.4 2,453,459 - Pf6N2E2_2061 0.19 -2.3 2,453,648 - Pf6N2E2_2061 0.36 +0.2 2,454,021 + Pf6N2E2_2061 0.71 -0.7 2,454,022 - Pf6N2E2_2061 0.72 -1.3 2,454,022 - Pf6N2E2_2061 0.72 -0.3 2,454,116 + Pf6N2E2_2061 0.80 -0.7 2,454,116 + Pf6N2E2_2061 0.80 -0.7 2,454,116 + Pf6N2E2_2061 0.80 -1.0 2,454,117 - Pf6N2E2_2061 0.80 -0.6 2,454,422 - -0.5 2,454,422 - -0.2 2,454,422 - -0.2 2,454,422 - -0.0 2,454,510 + -1.0 2,454,511 - -0.9 2,454,595 + +0.2 2,454,595 + -0.8 2,454,596 - -2.7 2,454,596 - +0.3 2,454,635 + -0.8 2,454,636 - +1.1 2,454,670 + -0.1 2,454,670 + +0.4 2,454,670 + +0.2 2,454,671 - -1.0 2,454,671 - -1.2 2,454,671 - -0.7 2,454,671 - -0.8 2,454,671 - +0.6 2,454,671 - -0.9 2,454,739 + -0.3 2,454,739 + +0.3 2,454,805 + +0.7 2,454,805 + -1.6 2,454,805 + -1.0 2,454,805 + +0.2 2,454,805 + -3.5 2,454,805 + -0.3 2,454,806 - +0.0 2,454,806 - +0.5 2,454,806 - -0.7 2,454,806 - -1.0 2,454,846 + +0.3 2,454,846 + +0.5 2,454,851 - -1.1 2,454,900 + -0.1 2,454,901 - -0.5 2,455,194 + Pf6N2E2_2062 0.19 -0.2 2,455,195 - Pf6N2E2_2062 0.19 -0.4
Or see this region's nucleotide sequence