Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_96 and Pf6N2E2_97 are separated by 93 nucleotides Pf6N2E2_97 and Pf6N2E2_98 overlap by 4 nucleotides Pf6N2E2_98 and Pf6N2E2_99 are separated by 53 nucleotides Pf6N2E2_99 and Pf6N2E2_100 are separated by 298 nucleotides
Pf6N2E2_96 - FIG011065: hypothetical protein, at 141,787 to 142,551
_96
Pf6N2E2_97 - putataive branched-chain amino acid transport protein AzlC, at 142,645 to 143,355
_97
Pf6N2E2_98 - putative transmembrane protein, at 143,352 to 143,660
_98
Pf6N2E2_99 - hypothetical protein, at 143,714 to 143,854
_99
Pf6N2E2_100 - membrane protein, putative, at 144,153 to 145,364
_100
Position (kb)
143
144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.391 kb on - strand, within Pf6N2E2_96 at 142.528 kb on + strand at 142.528 kb on + strand at 142.528 kb on + strand at 142.528 kb on + strand at 142.529 kb on - strand at 142.560 kb on + strand at 142.602 kb on - strand at 142.602 kb on - strand at 142.602 kb on - strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.789 kb on - strand, within Pf6N2E2_97 at 142.800 kb on - strand, within Pf6N2E2_97 at 143.230 kb on - strand, within Pf6N2E2_97 at 143.392 kb on + strand, within Pf6N2E2_98 at 143.393 kb on - strand, within Pf6N2E2_98 at 143.629 kb on + strand, within Pf6N2E2_98 at 143.630 kb on - strand at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.768 kb on - strand, within Pf6N2E2_99 at 143.870 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.924 kb on - strand at 143.924 kb on - strand at 143.954 kb on - strand at 143.954 kb on - strand at 143.976 kb on - strand at 144.117 kb on + strand at 144.117 kb on + strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.128 kb on + strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.173 kb on - strand at 144.173 kb on - strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.276 kb on - strand, within Pf6N2E2_100 at 144.300 kb on + strand, within Pf6N2E2_100 at 144.403 kb on + strand, within Pf6N2E2_100 at 144.404 kb on - strand, within Pf6N2E2_100 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.600 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.631 kb on + strand, within Pf6N2E2_100
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 142,390 + Pf6N2E2_96 0.79 +0.1 142,390 + Pf6N2E2_96 0.79 -1.1 142,390 + Pf6N2E2_96 0.79 +0.7 142,391 - Pf6N2E2_96 0.79 -0.2 142,528 + +0.6 142,528 + -0.4 142,528 + -0.1 142,528 + +0.5 142,529 - +0.1 142,560 + +0.1 142,602 - +2.7 142,602 - +0.7 142,602 - -3.6 142,621 + -0.0 142,621 + -0.8 142,621 + +0.7 142,621 + -1.3 142,622 - +2.1 142,622 - -2.5 142,622 - +1.9 142,622 - +0.3 142,622 - -0.3 142,622 - +2.0 142,622 - +1.2 142,693 + +1.5 142,693 + -0.4 142,693 + -1.2 142,693 + +0.3 142,693 + -2.5 142,693 + +1.5 142,694 - -1.9 142,694 - -2.1 142,694 - -2.7 142,694 - +1.3 142,788 + Pf6N2E2_97 0.20 -1.8 142,788 + Pf6N2E2_97 0.20 -0.1 142,788 + Pf6N2E2_97 0.20 -1.0 142,788 + Pf6N2E2_97 0.20 +0.5 142,789 - Pf6N2E2_97 0.20 +0.8 142,800 - Pf6N2E2_97 0.22 -2.5 143,230 - Pf6N2E2_97 0.82 +1.1 143,392 + Pf6N2E2_98 0.13 -1.1 143,393 - Pf6N2E2_98 0.13 -0.2 143,629 + Pf6N2E2_98 0.90 -1.3 143,630 - -0.3 143,757 - Pf6N2E2_99 0.30 -0.1 143,757 - Pf6N2E2_99 0.30 -0.5 143,757 - Pf6N2E2_99 0.30 +0.1 143,768 - Pf6N2E2_99 0.38 +0.3 143,870 + -0.9 143,923 + +1.3 143,923 + -1.1 143,923 + -2.9 143,923 + +0.3 143,923 + -2.8 143,923 + -1.5 143,924 - -3.1 143,924 - -3.2 143,954 - -1.8 143,954 - +0.6 143,976 - +0.2 144,117 + -0.1 144,117 + +0.8 144,118 - -1.3 144,118 - +1.8 144,118 - +1.1 144,118 - +1.1 144,128 + +0.7 144,129 - -0.7 144,129 - +1.9 144,129 - -0.6 144,129 - -1.6 144,132 - +1.1 144,132 - -1.3 144,132 - -2.3 144,132 - -0.9 144,132 - +0.4 144,172 + +0.3 144,172 + +1.4 144,172 + +0.6 144,173 - -0.7 144,173 - +0.5 144,196 + -0.8 144,196 + -3.1 144,196 + -0.9 144,197 - +0.4 144,197 - -0.1 144,197 - -1.4 144,276 - Pf6N2E2_100 0.10 -1.5 144,300 + Pf6N2E2_100 0.12 +1.0 144,403 + Pf6N2E2_100 0.21 +0.9 144,404 - Pf6N2E2_100 0.21 -2.8 144,511 + Pf6N2E2_100 0.30 +0.8 144,511 + Pf6N2E2_100 0.30 +0.6 144,512 - Pf6N2E2_100 0.30 -1.6 144,512 - Pf6N2E2_100 0.30 +0.5 144,600 + Pf6N2E2_100 0.37 +0.0 144,610 + Pf6N2E2_100 0.38 -0.4 144,610 + Pf6N2E2_100 0.38 -1.5 144,610 + Pf6N2E2_100 0.38 +0.8 144,631 + Pf6N2E2_100 0.39 -1.9
Or see this region's nucleotide sequence