Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5153 and Pf6N2E2_5154 are separated by 275 nucleotides Pf6N2E2_5154 and Pf6N2E2_5155 are separated by 3 nucleotides Pf6N2E2_5155 and Pf6N2E2_5156 are separated by 718 nucleotides
Pf6N2E2_5153 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 5,940,125 to 5,940,925
_5153
Pf6N2E2_5154 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 5,941,201 to 5,941,686
_5154
Pf6N2E2_5155 - Poly(A) polymerase (EC 2.7.7.19), at 5,941,690 to 5,943,087
_5155
Pf6N2E2_5156 - Two-component sensor CbrB: intrcellular carbon:nitrogen balance, at 5,943,806 to 5,945,239
_5156
Position (kb)
5941
5942
5943
5944 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5940.726 kb on + strand, within Pf6N2E2_5153 at 5940.727 kb on - strand, within Pf6N2E2_5153 at 5940.853 kb on - strand at 5940.853 kb on - strand at 5940.853 kb on - strand at 5940.914 kb on - strand at 5940.916 kb on + strand at 5940.916 kb on + strand at 5940.916 kb on + strand at 5940.916 kb on + strand at 5940.917 kb on - strand at 5940.917 kb on - strand at 5940.917 kb on - strand at 5940.917 kb on - strand at 5940.940 kb on + strand at 5940.941 kb on - strand at 5940.955 kb on + strand at 5940.956 kb on - strand at 5940.965 kb on - strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.988 kb on - strand at 5941.002 kb on + strand at 5941.002 kb on + strand at 5941.002 kb on + strand at 5941.002 kb on + strand at 5941.003 kb on - strand at 5941.003 kb on - strand at 5941.003 kb on - strand at 5941.003 kb on - strand at 5941.017 kb on + strand at 5941.018 kb on - strand at 5941.018 kb on - strand at 5941.018 kb on - strand at 5941.069 kb on - strand at 5941.069 kb on - strand at 5941.069 kb on - strand at 5941.070 kb on + strand at 5941.070 kb on + strand at 5941.070 kb on + strand at 5941.071 kb on - strand at 5941.071 kb on - strand at 5941.071 kb on - strand at 5941.071 kb on - strand at 5941.130 kb on + strand at 5941.206 kb on + strand at 5941.674 kb on - strand at 5941.674 kb on - strand at 5942.235 kb on - strand, within Pf6N2E2_5155 at 5942.462 kb on - strand, within Pf6N2E2_5155 at 5942.592 kb on - strand, within Pf6N2E2_5155 at 5942.595 kb on - strand, within Pf6N2E2_5155 at 5942.830 kb on + strand, within Pf6N2E2_5155 at 5942.919 kb on - strand, within Pf6N2E2_5155 at 5942.919 kb on - strand, within Pf6N2E2_5155 at 5943.110 kb on - strand at 5943.154 kb on + strand at 5943.154 kb on + strand at 5943.155 kb on - strand at 5943.161 kb on + strand at 5943.161 kb on + strand at 5943.223 kb on + strand at 5943.224 kb on - strand at 5943.282 kb on + strand at 5943.283 kb on - strand at 5943.287 kb on - strand at 5943.302 kb on + strand at 5943.306 kb on - strand at 5943.468 kb on - strand at 5943.468 kb on - strand at 5943.503 kb on + strand at 5943.504 kb on - strand at 5943.504 kb on - strand at 5943.504 kb on - strand at 5943.504 kb on - strand at 5943.529 kb on + strand at 5943.530 kb on - strand at 5943.557 kb on - strand at 5943.581 kb on - strand at 5943.586 kb on + strand at 5943.608 kb on + strand at 5943.619 kb on + strand at 5943.628 kb on + strand at 5943.628 kb on + strand at 5943.629 kb on - strand at 5943.686 kb on + strand at 5943.686 kb on + strand at 5943.687 kb on - strand at 5943.688 kb on + strand at 5943.689 kb on - strand at 5943.732 kb on + strand at 5943.733 kb on - strand at 5943.733 kb on - strand at 5943.750 kb on - strand at 5943.768 kb on - strand at 5943.768 kb on - strand at 5943.955 kb on - strand, within Pf6N2E2_5156
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 5,940,726 + Pf6N2E2_5153 0.75 +1.0 5,940,727 - Pf6N2E2_5153 0.75 -1.3 5,940,853 - +0.0 5,940,853 - +1.1 5,940,853 - +1.0 5,940,914 - -0.5 5,940,916 + -2.2 5,940,916 + +1.3 5,940,916 + -0.9 5,940,916 + -0.5 5,940,917 - +0.9 5,940,917 - -0.8 5,940,917 - +1.6 5,940,917 - +0.3 5,940,940 + +0.4 5,940,941 - -0.1 5,940,955 + -0.9 5,940,956 - -0.4 5,940,965 - +1.2 5,940,985 + -1.0 5,940,985 + -0.6 5,940,985 + +1.3 5,940,985 + -0.7 5,940,985 + +0.9 5,940,985 + -0.6 5,940,985 + -0.3 5,940,986 - +0.9 5,940,986 - +2.1 5,940,986 - +1.6 5,940,986 - -1.2 5,940,986 - +1.7 5,940,986 - -1.9 5,940,986 - +0.1 5,940,986 - +2.8 5,940,986 - +1.3 5,940,986 - +0.7 5,940,986 - -0.0 5,940,986 - -0.5 5,940,988 - +1.1 5,941,002 + -0.1 5,941,002 + -2.2 5,941,002 + -0.6 5,941,002 + +1.1 5,941,003 - -0.7 5,941,003 - +1.0 5,941,003 - -0.5 5,941,003 - -0.6 5,941,017 + +0.6 5,941,018 - +0.1 5,941,018 - +0.6 5,941,018 - -0.9 5,941,069 - +2.0 5,941,069 - +0.8 5,941,069 - +1.1 5,941,070 + -0.1 5,941,070 + +0.5 5,941,070 + -3.1 5,941,071 - +0.6 5,941,071 - -2.1 5,941,071 - +0.4 5,941,071 - +0.3 5,941,130 + -0.9 5,941,206 + -0.0 5,941,674 - -0.3 5,941,674 - +0.4 5,942,235 - Pf6N2E2_5155 0.39 -1.3 5,942,462 - Pf6N2E2_5155 0.55 -2.9 5,942,592 - Pf6N2E2_5155 0.65 +1.6 5,942,595 - Pf6N2E2_5155 0.65 +0.4 5,942,830 + Pf6N2E2_5155 0.82 -1.6 5,942,919 - Pf6N2E2_5155 0.88 -1.9 5,942,919 - Pf6N2E2_5155 0.88 -2.1 5,943,110 - -0.6 5,943,154 + -0.5 5,943,154 + -0.3 5,943,155 - -0.9 5,943,161 + +0.1 5,943,161 + +1.8 5,943,223 + -1.6 5,943,224 - -2.2 5,943,282 + -2.3 5,943,283 - -0.5 5,943,287 - +0.1 5,943,302 + +1.7 5,943,306 - -0.4 5,943,468 - -0.7 5,943,468 - -0.7 5,943,503 + +0.4 5,943,504 - +1.4 5,943,504 - -0.0 5,943,504 - +0.4 5,943,504 - +0.4 5,943,529 + -1.8 5,943,530 - +1.0 5,943,557 - +0.0 5,943,581 - +0.5 5,943,586 + -1.3 5,943,608 + +1.3 5,943,619 + -2.3 5,943,628 + +0.3 5,943,628 + +0.3 5,943,629 - +0.5 5,943,686 + +1.9 5,943,686 + -0.9 5,943,687 - +1.2 5,943,688 + -0.0 5,943,689 - -1.1 5,943,732 + +1.2 5,943,733 - +0.1 5,943,733 - +0.5 5,943,750 - +2.1 5,943,768 - +1.4 5,943,768 - +1.1 5,943,955 - Pf6N2E2_5156 0.10 -1.6
Or see this region's nucleotide sequence