Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4526 and Pf6N2E2_4527 overlap by 4 nucleotides Pf6N2E2_4527 and Pf6N2E2_4528 are separated by 226 nucleotides Pf6N2E2_4528 and Pf6N2E2_4529 overlap by 30 nucleotides Pf6N2E2_4529 and Pf6N2E2_4530 overlap by 4 nucleotides
Pf6N2E2_4526 - Phosphinothricin N-acetyltransferase (EC 2.3.1.-), at 5,261,276 to 5,261,872
_4526
Pf6N2E2_4527 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,261,869 to 5,262,420
_4527
Pf6N2E2_4528 - hypothetical protein, at 5,262,647 to 5,262,760
_4528
Pf6N2E2_4529 - type III secretion protein HrpJ, at 5,262,731 to 5,263,858
_4529
Pf6N2E2_4530 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 5,263,855 to 5,265,942
_4530
Position (kb) 5262
5263
5264 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5261.758 kb on + strand, within Pf6N2E2_4526 at 5261.758 kb on + strand, within Pf6N2E2_4526 at 5261.759 kb on - strand, within Pf6N2E2_4526 at 5261.786 kb on - strand, within Pf6N2E2_4526 at 5261.786 kb on - strand, within Pf6N2E2_4526 at 5261.888 kb on + strand at 5262.158 kb on + strand, within Pf6N2E2_4527 at 5262.159 kb on - strand, within Pf6N2E2_4527 at 5262.159 kb on - strand, within Pf6N2E2_4527 at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.439 kb on - strand at 5262.514 kb on - strand at 5262.556 kb on + strand at 5262.738 kb on + strand at 5262.738 kb on + strand at 5262.739 kb on - strand at 5262.739 kb on - strand at 5262.739 kb on - strand at 5262.760 kb on + strand at 5262.892 kb on + strand, within Pf6N2E2_4529 at 5262.893 kb on - strand, within Pf6N2E2_4529 at 5262.893 kb on - strand, within Pf6N2E2_4529 at 5263.197 kb on + strand at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.305 kb on - strand, within Pf6N2E2_4529 at 5263.307 kb on + strand, within Pf6N2E2_4529 at 5263.307 kb on + strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.552 kb on + strand, within Pf6N2E2_4529 at 5263.552 kb on + strand, within Pf6N2E2_4529 at 5263.638 kb on + strand, within Pf6N2E2_4529 at 5263.638 kb on + strand, within Pf6N2E2_4529 at 5263.639 kb on - strand, within Pf6N2E2_4529 at 5263.645 kb on + strand, within Pf6N2E2_4529 at 5263.646 kb on - strand, within Pf6N2E2_4529 at 5263.706 kb on + strand, within Pf6N2E2_4529 at 5264.032 kb on + strand at 5264.033 kb on - strand at 5264.551 kb on + strand, within Pf6N2E2_4530 at 5264.552 kb on - strand, within Pf6N2E2_4530 at 5264.552 kb on - strand, within Pf6N2E2_4530 at 5264.552 kb on - strand, within Pf6N2E2_4530 at 5264.613 kb on + strand, within Pf6N2E2_4530 at 5264.614 kb on - strand, within Pf6N2E2_4530 at 5264.828 kb on - strand, within Pf6N2E2_4530
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 5,261,758 + Pf6N2E2_4526 0.81 +0.4 5,261,758 + Pf6N2E2_4526 0.81 -0.3 5,261,759 - Pf6N2E2_4526 0.81 -2.0 5,261,786 - Pf6N2E2_4526 0.85 +0.5 5,261,786 - Pf6N2E2_4526 0.85 +0.1 5,261,888 + -0.3 5,262,158 + Pf6N2E2_4527 0.52 -2.7 5,262,159 - Pf6N2E2_4527 0.53 +1.0 5,262,159 - Pf6N2E2_4527 0.53 +0.3 5,262,424 + -1.2 5,262,424 + +1.2 5,262,424 + +0.6 5,262,424 + -0.2 5,262,425 - +1.0 5,262,425 - +1.5 5,262,425 - -2.3 5,262,425 - -0.6 5,262,439 - -0.3 5,262,514 - +1.3 5,262,556 + -0.3 5,262,738 + +0.4 5,262,738 + +0.0 5,262,739 - -0.2 5,262,739 - +0.7 5,262,739 - +0.8 5,262,760 + -3.3 5,262,892 + Pf6N2E2_4529 0.14 -0.8 5,262,893 - Pf6N2E2_4529 0.14 +1.4 5,262,893 - Pf6N2E2_4529 0.14 +0.3 5,263,197 + +1.5 5,263,198 - Pf6N2E2_4529 0.41 -1.0 5,263,198 - Pf6N2E2_4529 0.41 -0.9 5,263,198 - Pf6N2E2_4529 0.41 -0.9 5,263,304 + Pf6N2E2_4529 0.51 -0.3 5,263,304 + Pf6N2E2_4529 0.51 +0.3 5,263,304 + Pf6N2E2_4529 0.51 +1.1 5,263,305 - Pf6N2E2_4529 0.51 +0.5 5,263,307 + Pf6N2E2_4529 0.51 -0.4 5,263,307 + Pf6N2E2_4529 0.51 -0.2 5,263,308 - Pf6N2E2_4529 0.51 -3.6 5,263,308 - Pf6N2E2_4529 0.51 -3.2 5,263,308 - Pf6N2E2_4529 0.51 -0.7 5,263,552 + Pf6N2E2_4529 0.73 +0.7 5,263,552 + Pf6N2E2_4529 0.73 -0.7 5,263,638 + Pf6N2E2_4529 0.80 +0.2 5,263,638 + Pf6N2E2_4529 0.80 +0.7 5,263,639 - Pf6N2E2_4529 0.80 -1.3 5,263,645 + Pf6N2E2_4529 0.81 -0.7 5,263,646 - Pf6N2E2_4529 0.81 +0.3 5,263,706 + Pf6N2E2_4529 0.86 +0.1 5,264,032 + +0.8 5,264,033 - +0.3 5,264,551 + Pf6N2E2_4530 0.33 -0.2 5,264,552 - Pf6N2E2_4530 0.33 +0.1 5,264,552 - Pf6N2E2_4530 0.33 -1.6 5,264,552 - Pf6N2E2_4530 0.33 -0.0 5,264,613 + Pf6N2E2_4530 0.36 -0.2 5,264,614 - Pf6N2E2_4530 0.36 +0.2 5,264,828 - Pf6N2E2_4530 0.47 -0.2
Or see this region's nucleotide sequence