Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3349 and Pf6N2E2_3350 are separated by 194 nucleotides Pf6N2E2_3350 and Pf6N2E2_3351 are separated by 143 nucleotides Pf6N2E2_3351 and Pf6N2E2_3352 are separated by 4 nucleotides Pf6N2E2_3352 and Pf6N2E2_3353 are separated by 159 nucleotides
Pf6N2E2_3349 - Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), at 3,877,057 to 3,879,162
_3349
Pf6N2E2_3350 - SSU ribosomal protein S15p (S13e), at 3,879,357 to 3,879,626
_3350
Pf6N2E2_3351 - tRNA pseudouridine synthase B (EC 4.2.1.70), at 3,879,770 to 3,880,687
_3351
Pf6N2E2_3352 - Ribosome-binding factor A, at 3,880,692 to 3,881,093
_3352
Pf6N2E2_3353 - Translation initiation factor 2, at 3,881,253 to 3,883,772
_3353
Position (kb)
3879
3880
3881 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3879.272 kb on - strand at 3879.272 kb on - strand at 3879.848 kb on - strand at 3879.897 kb on - strand, within Pf6N2E2_3351 at 3879.919 kb on - strand, within Pf6N2E2_3351 at 3880.102 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.190 kb on - strand, within Pf6N2E2_3351 at 3880.193 kb on - strand, within Pf6N2E2_3351 at 3880.229 kb on - strand, within Pf6N2E2_3351 at 3880.253 kb on + strand, within Pf6N2E2_3351 at 3880.276 kb on + strand, within Pf6N2E2_3351 at 3880.276 kb on + strand, within Pf6N2E2_3351 at 3880.277 kb on - strand, within Pf6N2E2_3351 at 3880.278 kb on + strand, within Pf6N2E2_3351 at 3880.311 kb on + strand, within Pf6N2E2_3351
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 3,879,272 - +1.4 3,879,272 - -1.0 3,879,848 - -1.0 3,879,897 - Pf6N2E2_3351 0.14 -1.5 3,879,919 - Pf6N2E2_3351 0.16 -1.4 3,880,102 - Pf6N2E2_3351 0.36 -1.9 3,880,153 - Pf6N2E2_3351 0.42 +0.3 3,880,153 - Pf6N2E2_3351 0.42 +0.1 3,880,153 - Pf6N2E2_3351 0.42 -0.7 3,880,153 - Pf6N2E2_3351 0.42 -1.7 3,880,190 - Pf6N2E2_3351 0.46 -3.0 3,880,193 - Pf6N2E2_3351 0.46 -2.9 3,880,229 - Pf6N2E2_3351 0.50 -1.5 3,880,253 + Pf6N2E2_3351 0.53 -2.2 3,880,276 + Pf6N2E2_3351 0.55 +0.6 3,880,276 + Pf6N2E2_3351 0.55 +0.3 3,880,277 - Pf6N2E2_3351 0.55 -1.3 3,880,278 + Pf6N2E2_3351 0.55 -2.5 3,880,311 + Pf6N2E2_3351 0.59 -1.4
Or see this region's nucleotide sequence