Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_303 and Pf6N2E2_304 are separated by 88 nucleotides Pf6N2E2_304 and Pf6N2E2_305 overlap by 4 nucleotides Pf6N2E2_305 and Pf6N2E2_306 overlap by 4 nucleotides Pf6N2E2_306 and Pf6N2E2_307 overlap by 1 nucleotides Pf6N2E2_307 and Pf6N2E2_308 overlap by 1 nucleotides
Pf6N2E2_303 - Glutathione S-transferase, omega (EC 2.5.1.18), at 373,917 to 374,915
_303
Pf6N2E2_304 - Anthranilate phosphoribosyltransferase like (EC 2.4.2.18), at 375,004 to 376,005
_304
Pf6N2E2_305 - tRNA 2-thiouridine synthesizing protein E (EC 2.8.1.-), at 376,002 to 376,337
_305
Pf6N2E2_306 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusB, at 376,334 to 376,627
_306
Pf6N2E2_307 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusC, at 376,627 to 376,989
_307
Pf6N2E2_308 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 376,989 to 377,381
_308
Position (kb) 375
376
377 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.052 kb on - strand, within Pf6N2E2_303 at 374.092 kb on + strand, within Pf6N2E2_303 at 374.167 kb on + strand, within Pf6N2E2_303 at 374.167 kb on + strand, within Pf6N2E2_303 at 374.168 kb on - strand, within Pf6N2E2_303 at 374.225 kb on - strand, within Pf6N2E2_303 at 374.225 kb on - strand, within Pf6N2E2_303 at 374.239 kb on + strand, within Pf6N2E2_303 at 374.308 kb on + strand, within Pf6N2E2_303 at 374.309 kb on - strand, within Pf6N2E2_303 at 374.366 kb on - strand, within Pf6N2E2_303 at 374.545 kb on + strand, within Pf6N2E2_303 at 374.546 kb on - strand, within Pf6N2E2_303 at 374.566 kb on + strand, within Pf6N2E2_303 at 374.656 kb on + strand, within Pf6N2E2_303 at 374.670 kb on + strand, within Pf6N2E2_303 at 374.670 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.875 kb on + strand at 374.875 kb on + strand at 374.875 kb on + strand at 374.876 kb on - strand at 374.876 kb on - strand at 374.904 kb on + strand at 374.953 kb on + strand at 374.973 kb on - strand at 375.005 kb on + strand at 375.032 kb on + strand at 375.123 kb on - strand, within Pf6N2E2_304 at 375.563 kb on - strand, within Pf6N2E2_304 at 375.599 kb on + strand, within Pf6N2E2_304 at 375.684 kb on - strand, within Pf6N2E2_304 at 375.713 kb on + strand, within Pf6N2E2_304 at 375.713 kb on + strand, within Pf6N2E2_304 at 375.820 kb on + strand, within Pf6N2E2_304
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 374,035 + Pf6N2E2_303 0.12 -0.2 374,035 + Pf6N2E2_303 0.12 -0.0 374,035 + Pf6N2E2_303 0.12 -0.5 374,036 - Pf6N2E2_303 0.12 -1.5 374,036 - Pf6N2E2_303 0.12 +1.3 374,036 - Pf6N2E2_303 0.12 +1.6 374,036 - Pf6N2E2_303 0.12 +0.3 374,039 - Pf6N2E2_303 0.12 +0.2 374,039 - Pf6N2E2_303 0.12 -0.0 374,039 - Pf6N2E2_303 0.12 +0.1 374,052 - Pf6N2E2_303 0.14 +0.6 374,092 + Pf6N2E2_303 0.18 +1.7 374,167 + Pf6N2E2_303 0.25 +1.0 374,167 + Pf6N2E2_303 0.25 +0.5 374,168 - Pf6N2E2_303 0.25 +1.3 374,225 - Pf6N2E2_303 0.31 +0.2 374,225 - Pf6N2E2_303 0.31 +0.3 374,239 + Pf6N2E2_303 0.32 -1.7 374,308 + Pf6N2E2_303 0.39 -1.0 374,309 - Pf6N2E2_303 0.39 -3.0 374,366 - Pf6N2E2_303 0.45 -1.6 374,545 + Pf6N2E2_303 0.63 +0.7 374,546 - Pf6N2E2_303 0.63 +0.4 374,566 + Pf6N2E2_303 0.65 +0.4 374,656 + Pf6N2E2_303 0.74 -0.5 374,670 + Pf6N2E2_303 0.75 +1.7 374,670 + Pf6N2E2_303 0.75 +0.9 374,749 + Pf6N2E2_303 0.83 -0.4 374,749 + Pf6N2E2_303 0.83 -0.3 374,749 + Pf6N2E2_303 0.83 -3.3 374,749 + Pf6N2E2_303 0.83 -1.3 374,749 + Pf6N2E2_303 0.83 +0.2 374,749 + Pf6N2E2_303 0.83 -3.9 374,749 + Pf6N2E2_303 0.83 -0.2 374,749 + Pf6N2E2_303 0.83 -1.0 374,749 + Pf6N2E2_303 0.83 +1.3 374,749 + Pf6N2E2_303 0.83 +0.7 374,750 - Pf6N2E2_303 0.83 -0.5 374,750 - Pf6N2E2_303 0.83 -0.6 374,750 - Pf6N2E2_303 0.83 +1.4 374,750 - Pf6N2E2_303 0.83 +0.3 374,750 - Pf6N2E2_303 0.83 +0.9 374,750 - Pf6N2E2_303 0.83 -2.8 374,875 + +0.2 374,875 + -0.7 374,875 + -0.5 374,876 - +0.0 374,876 - -0.8 374,904 + +1.4 374,953 + -0.4 374,973 - +0.6 375,005 + -0.5 375,032 + +1.4 375,123 - Pf6N2E2_304 0.12 +0.4 375,563 - Pf6N2E2_304 0.56 -1.7 375,599 + Pf6N2E2_304 0.59 -2.2 375,684 - Pf6N2E2_304 0.68 -0.2 375,713 + Pf6N2E2_304 0.71 -1.4 375,713 + Pf6N2E2_304 0.71 -0.7 375,820 + Pf6N2E2_304 0.81 -0.4
Or see this region's nucleotide sequence