Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_301 and Pf6N2E2_302 are separated by 0 nucleotides Pf6N2E2_302 and Pf6N2E2_303 are separated by 127 nucleotides
Pf6N2E2_301 - Seryl-tRNA synthetase (EC 6.1.1.11), at 371,114 to 372,394
_301
Pf6N2E2_302 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 372,395 to 373,789
_302
Pf6N2E2_303 - Glutathione S-transferase, omega (EC 2.5.1.18), at 373,917 to 374,915
_303
Position (kb) 372
373
374 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 372.401 kb on + strand at 372.587 kb on + strand, within Pf6N2E2_302 at 372.588 kb on - strand, within Pf6N2E2_302 at 373.049 kb on + strand, within Pf6N2E2_302 at 373.193 kb on + strand, within Pf6N2E2_302 at 373.193 kb on + strand, within Pf6N2E2_302 at 373.440 kb on - strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.539 kb on - strand, within Pf6N2E2_302 at 373.539 kb on - strand, within Pf6N2E2_302 at 373.539 kb on - strand, within Pf6N2E2_302 at 373.539 kb on - strand, within Pf6N2E2_302 at 373.755 kb on + strand at 373.755 kb on + strand at 373.787 kb on + strand at 373.788 kb on - strand at 374.035 kb on + strand, within Pf6N2E2_303 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.052 kb on - strand, within Pf6N2E2_303 at 374.092 kb on + strand, within Pf6N2E2_303 at 374.167 kb on + strand, within Pf6N2E2_303 at 374.167 kb on + strand, within Pf6N2E2_303 at 374.168 kb on - strand, within Pf6N2E2_303 at 374.225 kb on - strand, within Pf6N2E2_303 at 374.225 kb on - strand, within Pf6N2E2_303 at 374.239 kb on + strand, within Pf6N2E2_303 at 374.308 kb on + strand, within Pf6N2E2_303 at 374.309 kb on - strand, within Pf6N2E2_303 at 374.366 kb on - strand, within Pf6N2E2_303 at 374.545 kb on + strand, within Pf6N2E2_303 at 374.546 kb on - strand, within Pf6N2E2_303 at 374.566 kb on + strand, within Pf6N2E2_303 at 374.656 kb on + strand, within Pf6N2E2_303 at 374.670 kb on + strand, within Pf6N2E2_303 at 374.670 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 372,401 + -0.2 372,587 + Pf6N2E2_302 0.14 -3.8 372,588 - Pf6N2E2_302 0.14 -2.8 373,049 + Pf6N2E2_302 0.47 -3.0 373,193 + Pf6N2E2_302 0.57 -2.6 373,193 + Pf6N2E2_302 0.57 -3.5 373,440 - Pf6N2E2_302 0.75 -0.0 373,538 + Pf6N2E2_302 0.82 -3.1 373,538 + Pf6N2E2_302 0.82 -1.6 373,538 + Pf6N2E2_302 0.82 -3.3 373,538 + Pf6N2E2_302 0.82 -2.7 373,538 + Pf6N2E2_302 0.82 -3.3 373,539 - Pf6N2E2_302 0.82 -2.8 373,539 - Pf6N2E2_302 0.82 -1.7 373,539 - Pf6N2E2_302 0.82 -1.9 373,539 - Pf6N2E2_302 0.82 -3.1 373,755 + -1.9 373,755 + -1.7 373,787 + -0.1 373,788 - -2.4 374,035 + Pf6N2E2_303 0.12 -0.2 374,035 + Pf6N2E2_303 0.12 -0.0 374,035 + Pf6N2E2_303 0.12 -0.5 374,036 - Pf6N2E2_303 0.12 -1.5 374,036 - Pf6N2E2_303 0.12 +1.3 374,036 - Pf6N2E2_303 0.12 +1.6 374,036 - Pf6N2E2_303 0.12 +0.3 374,039 - Pf6N2E2_303 0.12 +0.2 374,039 - Pf6N2E2_303 0.12 -0.0 374,039 - Pf6N2E2_303 0.12 +0.1 374,052 - Pf6N2E2_303 0.14 +0.6 374,092 + Pf6N2E2_303 0.18 +1.7 374,167 + Pf6N2E2_303 0.25 +1.0 374,167 + Pf6N2E2_303 0.25 +0.5 374,168 - Pf6N2E2_303 0.25 +1.3 374,225 - Pf6N2E2_303 0.31 +0.2 374,225 - Pf6N2E2_303 0.31 +0.3 374,239 + Pf6N2E2_303 0.32 -1.7 374,308 + Pf6N2E2_303 0.39 -1.0 374,309 - Pf6N2E2_303 0.39 -3.0 374,366 - Pf6N2E2_303 0.45 -1.6 374,545 + Pf6N2E2_303 0.63 +0.7 374,546 - Pf6N2E2_303 0.63 +0.4 374,566 + Pf6N2E2_303 0.65 +0.4 374,656 + Pf6N2E2_303 0.74 -0.5 374,670 + Pf6N2E2_303 0.75 +1.7 374,670 + Pf6N2E2_303 0.75 +0.9 374,749 + Pf6N2E2_303 0.83 -0.4 374,749 + Pf6N2E2_303 0.83 -0.3 374,749 + Pf6N2E2_303 0.83 -3.3 374,749 + Pf6N2E2_303 0.83 -1.3 374,749 + Pf6N2E2_303 0.83 +0.2 374,749 + Pf6N2E2_303 0.83 -3.9 374,749 + Pf6N2E2_303 0.83 -0.2 374,749 + Pf6N2E2_303 0.83 -1.0 374,749 + Pf6N2E2_303 0.83 +1.3 374,749 + Pf6N2E2_303 0.83 +0.7 374,750 - Pf6N2E2_303 0.83 -0.5 374,750 - Pf6N2E2_303 0.83 -0.6 374,750 - Pf6N2E2_303 0.83 +1.4 374,750 - Pf6N2E2_303 0.83 +0.3 374,750 - Pf6N2E2_303 0.83 +0.9 374,750 - Pf6N2E2_303 0.83 -2.8
Or see this region's nucleotide sequence