Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2365 and Pf6N2E2_2366 overlap by 8 nucleotides Pf6N2E2_2366 and Pf6N2E2_2367 are separated by 541 nucleotides Pf6N2E2_2367 and Pf6N2E2_2368 overlap by 4 nucleotides Pf6N2E2_2368 and Pf6N2E2_2369 overlap by 17 nucleotides
Pf6N2E2_2365 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 2,799,421 to 2,800,080
_2365
Pf6N2E2_2366 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 2,800,073 to 2,800,453
_2366
Pf6N2E2_2367 - hypothetical protein, at 2,800,995 to 2,801,870
_2367
Pf6N2E2_2368 - Surface presentation of antigens protein SpaM, at 2,801,867 to 2,802,322
_2368
Pf6N2E2_2369 - Flagellum-specific ATP synthase FliI, at 2,802,306 to 2,803,556
_2369
Position (kb)
2800
2801
2802 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2800.004 kb on + strand, within Pf6N2E2_2365 at 2800.005 kb on - strand, within Pf6N2E2_2365 at 2800.370 kb on + strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.384 kb on + strand, within Pf6N2E2_2366 at 2800.385 kb on - strand, within Pf6N2E2_2366 at 2800.878 kb on - strand at 2801.664 kb on - strand, within Pf6N2E2_2367 at 2801.664 kb on - strand, within Pf6N2E2_2367 at 2802.616 kb on + strand, within Pf6N2E2_2369 at 2802.617 kb on - strand, within Pf6N2E2_2369 at 2802.694 kb on + strand, within Pf6N2E2_2369 at 2802.695 kb on - strand, within Pf6N2E2_2369
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 2,800,004 + Pf6N2E2_2365 0.88 -3.5 2,800,005 - Pf6N2E2_2365 0.88 -0.1 2,800,370 + Pf6N2E2_2366 0.78 +1.0 2,800,371 - Pf6N2E2_2366 0.78 -1.4 2,800,371 - Pf6N2E2_2366 0.78 +1.3 2,800,371 - Pf6N2E2_2366 0.78 -1.3 2,800,371 - Pf6N2E2_2366 0.78 +1.5 2,800,384 + Pf6N2E2_2366 0.82 -0.0 2,800,385 - Pf6N2E2_2366 0.82 +0.5 2,800,878 - +0.9 2,801,664 - Pf6N2E2_2367 0.76 -0.7 2,801,664 - Pf6N2E2_2367 0.76 -1.5 2,802,616 + Pf6N2E2_2369 0.25 +1.4 2,802,617 - Pf6N2E2_2369 0.25 -0.5 2,802,694 + Pf6N2E2_2369 0.31 +0.8 2,802,695 - Pf6N2E2_2369 0.31 +0.2
Or see this region's nucleotide sequence