Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_227 and Pf6N2E2_228 are separated by 21 nucleotides Pf6N2E2_228 and Pf6N2E2_229 are separated by 2 nucleotides Pf6N2E2_229 and Pf6N2E2_230 are separated by 345 nucleotides
Pf6N2E2_227 - 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-), at 288,729 to 290,375
_227
Pf6N2E2_228 - Transcriptional regulator, IclR family, at 290,397 to 291,218
_228
Pf6N2E2_229 - Putative fumarylacetoacetate (FAA) hydrolase, at 291,221 to 292,072
_229
Pf6N2E2_230 - lignostilbene-alpha,beta-dioxygenase, at 292,418 to 293,866
_230
Position (kb)
291
292
293 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 290.379 kb on + strand at 290.379 kb on + strand at 290.582 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.801 kb on - strand, within Pf6N2E2_228 at 290.970 kb on - strand, within Pf6N2E2_228 at 290.993 kb on - strand, within Pf6N2E2_228 at 290.993 kb on - strand, within Pf6N2E2_228 at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.223 kb on - strand at 291.607 kb on - strand, within Pf6N2E2_229 at 291.607 kb on - strand, within Pf6N2E2_229 at 291.607 kb on - strand, within Pf6N2E2_229 at 291.784 kb on - strand, within Pf6N2E2_229 at 291.826 kb on - strand, within Pf6N2E2_229 at 292.032 kb on + strand at 292.032 kb on + strand at 292.032 kb on + strand at 292.032 kb on + strand at 292.033 kb on - strand at 292.036 kb on + strand at 292.037 kb on - strand at 292.037 kb on - strand at 292.039 kb on + strand at 292.055 kb on - strand at 292.055 kb on - strand at 292.110 kb on - strand at 292.196 kb on - strand at 292.196 kb on - strand at 292.337 kb on + strand at 292.338 kb on - strand at 292.388 kb on + strand at 292.388 kb on + strand at 292.388 kb on + strand at 292.389 kb on - strand at 292.389 kb on - strand at 292.570 kb on - strand, within Pf6N2E2_230 at 292.570 kb on - strand, within Pf6N2E2_230 at 292.570 kb on - strand, within Pf6N2E2_230 at 292.709 kb on + strand, within Pf6N2E2_230 at 292.710 kb on - strand, within Pf6N2E2_230 at 292.731 kb on + strand, within Pf6N2E2_230 at 292.732 kb on - strand, within Pf6N2E2_230 at 292.732 kb on - strand, within Pf6N2E2_230 at 292.789 kb on - strand, within Pf6N2E2_230 at 292.789 kb on - strand, within Pf6N2E2_230 at 292.821 kb on + strand, within Pf6N2E2_230 at 292.822 kb on - strand, within Pf6N2E2_230 at 292.822 kb on - strand, within Pf6N2E2_230 at 292.916 kb on + strand, within Pf6N2E2_230 at 292.916 kb on + strand, within Pf6N2E2_230 at 292.917 kb on - strand, within Pf6N2E2_230 at 292.920 kb on + strand, within Pf6N2E2_230 at 292.921 kb on - strand, within Pf6N2E2_230 at 292.944 kb on + strand, within Pf6N2E2_230 at 292.944 kb on + strand, within Pf6N2E2_230 at 292.945 kb on - strand, within Pf6N2E2_230 at 292.945 kb on - strand, within Pf6N2E2_230 at 292.945 kb on - strand, within Pf6N2E2_230 at 292.945 kb on - strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.981 kb on - strand, within Pf6N2E2_230 at 292.981 kb on - strand, within Pf6N2E2_230 at 293.014 kb on + strand, within Pf6N2E2_230 at 293.015 kb on - strand, within Pf6N2E2_230
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 290,379 + +1.1 290,379 + +1.3 290,582 - Pf6N2E2_228 0.23 -0.5 290,618 - Pf6N2E2_228 0.27 -0.1 290,618 - Pf6N2E2_228 0.27 -0.6 290,618 - Pf6N2E2_228 0.27 -2.4 290,618 - Pf6N2E2_228 0.27 +0.2 290,618 - Pf6N2E2_228 0.27 -0.6 290,618 - Pf6N2E2_228 0.27 -1.8 290,801 - Pf6N2E2_228 0.49 -2.7 290,970 - Pf6N2E2_228 0.70 -1.5 290,993 - Pf6N2E2_228 0.73 +1.1 290,993 - Pf6N2E2_228 0.73 -2.3 291,208 - -3.7 291,208 - +0.4 291,208 - +1.5 291,208 - +0.7 291,208 - -0.5 291,208 - +0.5 291,208 - -0.9 291,223 - -0.3 291,607 - Pf6N2E2_229 0.45 +0.3 291,607 - Pf6N2E2_229 0.45 +0.6 291,607 - Pf6N2E2_229 0.45 -3.0 291,784 - Pf6N2E2_229 0.66 -1.6 291,826 - Pf6N2E2_229 0.71 -0.9 292,032 + -3.5 292,032 + +0.6 292,032 + +0.5 292,032 + -1.7 292,033 - -0.7 292,036 + +1.5 292,037 - -0.5 292,037 - -0.3 292,039 + -0.7 292,055 - +0.6 292,055 - -1.6 292,110 - +1.2 292,196 - +0.1 292,196 - +0.4 292,337 + -1.5 292,338 - -3.3 292,388 + +1.3 292,388 + +4.0 292,388 + +1.2 292,389 - +1.7 292,389 - +1.1 292,570 - Pf6N2E2_230 0.10 -0.1 292,570 - Pf6N2E2_230 0.10 +1.3 292,570 - Pf6N2E2_230 0.10 +1.9 292,709 + Pf6N2E2_230 0.20 -0.6 292,710 - Pf6N2E2_230 0.20 +1.8 292,731 + Pf6N2E2_230 0.22 -0.5 292,732 - Pf6N2E2_230 0.22 +0.8 292,732 - Pf6N2E2_230 0.22 -1.0 292,789 - Pf6N2E2_230 0.26 -0.0 292,789 - Pf6N2E2_230 0.26 -0.1 292,821 + Pf6N2E2_230 0.28 -0.5 292,822 - Pf6N2E2_230 0.28 +0.2 292,822 - Pf6N2E2_230 0.28 +0.4 292,916 + Pf6N2E2_230 0.34 +1.4 292,916 + Pf6N2E2_230 0.34 +0.6 292,917 - Pf6N2E2_230 0.34 +0.5 292,920 + Pf6N2E2_230 0.35 +1.3 292,921 - Pf6N2E2_230 0.35 -2.2 292,944 + Pf6N2E2_230 0.36 +0.6 292,944 + Pf6N2E2_230 0.36 +0.5 292,945 - Pf6N2E2_230 0.36 -1.7 292,945 - Pf6N2E2_230 0.36 -0.6 292,945 - Pf6N2E2_230 0.36 +0.1 292,945 - Pf6N2E2_230 0.36 -0.1 292,980 + Pf6N2E2_230 0.39 -1.4 292,980 + Pf6N2E2_230 0.39 -2.0 292,980 + Pf6N2E2_230 0.39 -1.0 292,980 + Pf6N2E2_230 0.39 +1.9 292,980 + Pf6N2E2_230 0.39 +0.8 292,981 - Pf6N2E2_230 0.39 -0.4 292,981 - Pf6N2E2_230 0.39 +0.6 293,014 + Pf6N2E2_230 0.41 +0.4 293,015 - Pf6N2E2_230 0.41 +1.0
Or see this region's nucleotide sequence