Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2280 and Pf6N2E2_2281 are separated by 115 nucleotides Pf6N2E2_2281 and Pf6N2E2_2282 are separated by 171 nucleotides
Pf6N2E2_2280 - COG0553: Superfamily II DNA/RNA helicases, SNF2 family, at 2,697,766 to 2,700,459
_2280
Pf6N2E2_2281 - Beta N-acetyl-glucosaminidase (EC 3.2.1.52), at 2,700,575 to 2,701,585
_2281
Pf6N2E2_2282 - Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family, at 2,701,757 to 2,702,464
_2282
Position (kb)
2700
2701
2702 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2699.627 kb on + strand, within Pf6N2E2_2280 at 2699.627 kb on + strand, within Pf6N2E2_2280 at 2699.628 kb on - strand, within Pf6N2E2_2280 at 2699.655 kb on - strand, within Pf6N2E2_2280 at 2699.761 kb on + strand, within Pf6N2E2_2280 at 2699.761 kb on + strand, within Pf6N2E2_2280 at 2700.164 kb on + strand, within Pf6N2E2_2280 at 2700.164 kb on + strand, within Pf6N2E2_2280 at 2700.286 kb on + strand at 2700.287 kb on - strand at 2700.297 kb on - strand at 2700.577 kb on - strand at 2700.598 kb on + strand at 2700.654 kb on + strand at 2700.654 kb on + strand at 2700.655 kb on - strand at 2700.655 kb on - strand at 2700.655 kb on - strand at 2700.940 kb on - strand, within Pf6N2E2_2281 at 2700.940 kb on - strand, within Pf6N2E2_2281 at 2701.299 kb on + strand, within Pf6N2E2_2281 at 2701.585 kb on + strand at 2701.585 kb on + strand at 2701.585 kb on + strand at 2701.585 kb on + strand at 2701.585 kb on + strand at 2701.599 kb on + strand at 2701.600 kb on - strand at 2701.682 kb on + strand at 2701.683 kb on - strand at 2701.692 kb on + strand at 2701.718 kb on + strand at 2701.732 kb on + strand at 2701.732 kb on + strand at 2701.733 kb on - strand at 2701.733 kb on - strand at 2701.893 kb on - strand, within Pf6N2E2_2282 at 2701.893 kb on - strand, within Pf6N2E2_2282 at 2701.895 kb on - strand, within Pf6N2E2_2282 at 2701.895 kb on - strand, within Pf6N2E2_2282 at 2702.047 kb on - strand, within Pf6N2E2_2282 at 2702.083 kb on - strand, within Pf6N2E2_2282 at 2702.223 kb on - strand, within Pf6N2E2_2282 at 2702.223 kb on - strand, within Pf6N2E2_2282 at 2702.335 kb on - strand, within Pf6N2E2_2282 at 2702.499 kb on + strand at 2702.500 kb on - strand at 2702.500 kb on - strand at 2702.558 kb on + strand at 2702.559 kb on - strand at 2702.559 kb on - strand at 2702.559 kb on - strand at 2702.559 kb on - strand at 2702.563 kb on - strand at 2702.585 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 2,699,627 + Pf6N2E2_2280 0.69 +0.3 2,699,627 + Pf6N2E2_2280 0.69 -0.3 2,699,628 - Pf6N2E2_2280 0.69 +0.0 2,699,655 - Pf6N2E2_2280 0.70 -2.6 2,699,761 + Pf6N2E2_2280 0.74 +0.6 2,699,761 + Pf6N2E2_2280 0.74 -3.5 2,700,164 + Pf6N2E2_2280 0.89 -0.2 2,700,164 + Pf6N2E2_2280 0.89 +0.5 2,700,286 + +0.7 2,700,287 - -1.9 2,700,297 - +0.9 2,700,577 - -1.3 2,700,598 + -0.7 2,700,654 + +0.1 2,700,654 + +1.0 2,700,655 - +0.5 2,700,655 - -0.1 2,700,655 - -2.9 2,700,940 - Pf6N2E2_2281 0.36 -2.2 2,700,940 - Pf6N2E2_2281 0.36 -0.1 2,701,299 + Pf6N2E2_2281 0.72 -2.3 2,701,585 + -2.2 2,701,585 + -0.4 2,701,585 + -0.3 2,701,585 + +0.3 2,701,585 + -0.6 2,701,599 + +1.3 2,701,600 - -0.1 2,701,682 + +0.1 2,701,683 - -0.6 2,701,692 + +0.6 2,701,718 + -3.2 2,701,732 + +0.2 2,701,732 + +0.7 2,701,733 - -1.0 2,701,733 - -1.6 2,701,893 - Pf6N2E2_2282 0.19 -1.7 2,701,893 - Pf6N2E2_2282 0.19 +1.1 2,701,895 - Pf6N2E2_2282 0.19 -1.3 2,701,895 - Pf6N2E2_2282 0.19 -0.1 2,702,047 - Pf6N2E2_2282 0.41 +1.6 2,702,083 - Pf6N2E2_2282 0.46 +0.5 2,702,223 - Pf6N2E2_2282 0.66 -2.7 2,702,223 - Pf6N2E2_2282 0.66 -2.1 2,702,335 - Pf6N2E2_2282 0.82 -1.9 2,702,499 + +1.7 2,702,500 - +1.4 2,702,500 - -0.6 2,702,558 + -0.1 2,702,559 - +1.4 2,702,559 - -0.5 2,702,559 - -0.0 2,702,559 - +1.9 2,702,563 - +0.1 2,702,585 + -0.1
Or see this region's nucleotide sequence