Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1884 and Pf6N2E2_1885 are separated by 666 nucleotides Pf6N2E2_1885 and Pf6N2E2_1886 are separated by 639 nucleotides Pf6N2E2_1886 and Pf6N2E2_1887 are separated by 17 nucleotides Pf6N2E2_1887 and Pf6N2E2_1888 overlap by 47 nucleotides
Pf6N2E2_1884 - oxidoreductase, short-chain dehydrogenase/reductase family, at 2,235,697 to 2,236,422
_1884
Pf6N2E2_1885 - Transcriptional regulator, AraC family, at 2,237,089 to 2,237,466
_1885
Pf6N2E2_1886 - hypothetical protein, at 2,238,106 to 2,238,237
_1886
Pf6N2E2_1887 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 2,238,255 to 2,238,368
_1887
Pf6N2E2_1888 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 2,238,322 to 2,239,461
_1888
Position (kb)
2237
2238 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2236.393 kb on + strand at 2236.394 kb on - strand at 2236.464 kb on - strand at 2236.464 kb on - strand at 2236.464 kb on - strand at 2236.485 kb on - strand at 2236.496 kb on - strand at 2236.496 kb on - strand at 2236.501 kb on + strand at 2236.501 kb on + strand at 2236.677 kb on + strand at 2236.677 kb on + strand at 2236.768 kb on + strand at 2236.769 kb on - strand at 2236.783 kb on + strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.787 kb on + strand at 2236.788 kb on - strand at 2236.788 kb on - strand at 2236.815 kb on - strand at 2236.880 kb on + strand at 2236.881 kb on - strand at 2236.883 kb on + strand at 2236.884 kb on - strand at 2236.884 kb on - strand at 2236.934 kb on + strand at 2236.941 kb on - strand at 2236.941 kb on - strand at 2236.950 kb on - strand at 2236.950 kb on - strand at 2237.042 kb on + strand at 2237.060 kb on - strand at 2237.062 kb on - strand at 2237.062 kb on - strand at 2237.132 kb on + strand, within Pf6N2E2_1885 at 2237.132 kb on + strand, within Pf6N2E2_1885 at 2237.151 kb on - strand, within Pf6N2E2_1885 at 2237.270 kb on - strand, within Pf6N2E2_1885 at 2237.464 kb on + strand at 2237.570 kb on + strand at 2237.571 kb on - strand at 2237.571 kb on - strand at 2237.743 kb on + strand at 2237.744 kb on - strand at 2237.744 kb on - strand at 2237.772 kb on + strand at 2237.864 kb on + strand at 2237.865 kb on - strand at 2237.920 kb on + strand at 2237.921 kb on - strand at 2237.921 kb on - strand at 2238.026 kb on + strand at 2238.102 kb on - strand at 2238.102 kb on - strand at 2238.157 kb on - strand, within Pf6N2E2_1886 at 2238.157 kb on - strand, within Pf6N2E2_1886 at 2238.160 kb on - strand, within Pf6N2E2_1886 at 2238.236 kb on - strand at 2238.236 kb on - strand at 2238.364 kb on + strand at 2238.364 kb on + strand at 2238.364 kb on + strand at 2238.364 kb on + strand at 2238.365 kb on - strand at 2238.365 kb on - strand at 2238.412 kb on + strand at 2238.415 kb on + strand at 2238.449 kb on + strand, within Pf6N2E2_1888 at 2238.450 kb on - strand, within Pf6N2E2_1888 at 2238.450 kb on - strand, within Pf6N2E2_1888
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 2,236,393 + -1.0 2,236,394 - +0.2 2,236,464 - +1.1 2,236,464 - -2.3 2,236,464 - +0.0 2,236,485 - +1.3 2,236,496 - +0.3 2,236,496 - +0.5 2,236,501 + +0.0 2,236,501 + -1.1 2,236,677 + -2.5 2,236,677 + +1.0 2,236,768 + -3.9 2,236,769 - +0.2 2,236,783 + -0.1 2,236,784 - -0.5 2,236,784 - +1.3 2,236,784 - -1.2 2,236,784 - +1.1 2,236,784 - +1.3 2,236,787 + -0.2 2,236,788 - +0.7 2,236,788 - -2.3 2,236,815 - -1.7 2,236,880 + -0.9 2,236,881 - -0.1 2,236,883 + +1.4 2,236,884 - +0.9 2,236,884 - +0.7 2,236,934 + -0.1 2,236,941 - -3.2 2,236,941 - +0.7 2,236,950 - +0.0 2,236,950 - -0.1 2,237,042 + +1.3 2,237,060 - +0.9 2,237,062 - +0.3 2,237,062 - -2.5 2,237,132 + Pf6N2E2_1885 0.11 -2.2 2,237,132 + Pf6N2E2_1885 0.11 -0.5 2,237,151 - Pf6N2E2_1885 0.16 -1.1 2,237,270 - Pf6N2E2_1885 0.48 -2.4 2,237,464 + -1.9 2,237,570 + -1.3 2,237,571 - +1.0 2,237,571 - -0.2 2,237,743 + +0.1 2,237,744 - +0.5 2,237,744 - -1.9 2,237,772 + -0.6 2,237,864 + -1.1 2,237,865 - +1.0 2,237,920 + -0.3 2,237,921 - +1.8 2,237,921 - -0.0 2,238,026 + -0.5 2,238,102 - +1.1 2,238,102 - +0.6 2,238,157 - Pf6N2E2_1886 0.39 -2.5 2,238,157 - Pf6N2E2_1886 0.39 +0.5 2,238,160 - Pf6N2E2_1886 0.41 -2.1 2,238,236 - -0.6 2,238,236 - +0.8 2,238,364 + +0.7 2,238,364 + -0.5 2,238,364 + -0.5 2,238,364 + -0.0 2,238,365 - +0.7 2,238,365 - +0.1 2,238,412 + +2.1 2,238,415 + +0.7 2,238,449 + Pf6N2E2_1888 0.11 -0.1 2,238,450 - Pf6N2E2_1888 0.11 -1.4 2,238,450 - Pf6N2E2_1888 0.11 -1.1
Or see this region's nucleotide sequence