Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1543 and Pf6N2E2_1544 overlap by 11 nucleotides Pf6N2E2_1544 and Pf6N2E2_1545 are separated by 199 nucleotides Pf6N2E2_1545 and Pf6N2E2_1546 are separated by 164 nucleotides
Pf6N2E2_1543 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,801,238 to 1,802,719
_1543
Pf6N2E2_1544 - Thermostable hemolysin delta-VPH, at 1,802,709 to 1,803,386
_1544
Pf6N2E2_1545 - N-carbamoylputrescine amidase (EC 3.5.1.53), at 1,803,586 to 1,805,337
_1545
Pf6N2E2_1546 - Transcriptional regulator, LysR family, at 1,805,502 to 1,806,356
_1546
Position (kb)
1803
1804
1805
1806 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1802.593 kb on - strand at 1802.607 kb on + strand at 1802.986 kb on + strand, within Pf6N2E2_1544 at 1802.986 kb on + strand, within Pf6N2E2_1544 at 1802.986 kb on + strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1803.121 kb on + strand, within Pf6N2E2_1544 at 1803.121 kb on + strand, within Pf6N2E2_1544 at 1803.121 kb on + strand, within Pf6N2E2_1544 at 1803.422 kb on + strand at 1803.423 kb on - strand at 1803.423 kb on - strand at 1803.439 kb on - strand at 1803.523 kb on + strand at 1803.523 kb on + strand at 1803.718 kb on + strand at 1803.872 kb on - strand, within Pf6N2E2_1545 at 1803.889 kb on + strand, within Pf6N2E2_1545 at 1803.889 kb on + strand, within Pf6N2E2_1545 at 1803.889 kb on + strand, within Pf6N2E2_1545 at 1803.890 kb on - strand, within Pf6N2E2_1545 at 1804.087 kb on + strand, within Pf6N2E2_1545 at 1804.228 kb on - strand, within Pf6N2E2_1545 at 1804.498 kb on + strand, within Pf6N2E2_1545 at 1804.698 kb on + strand, within Pf6N2E2_1545 at 1804.698 kb on + strand, within Pf6N2E2_1545 at 1804.699 kb on - strand, within Pf6N2E2_1545 at 1804.703 kb on + strand, within Pf6N2E2_1545 at 1804.907 kb on - strand, within Pf6N2E2_1545 at 1804.907 kb on - strand, within Pf6N2E2_1545 at 1805.169 kb on + strand at 1805.243 kb on - strand at 1805.504 kb on - strand at 1805.515 kb on + strand at 1805.516 kb on - strand at 1805.955 kb on + strand, within Pf6N2E2_1546 at 1805.956 kb on - strand, within Pf6N2E2_1546 at 1806.049 kb on + strand, within Pf6N2E2_1546 at 1806.050 kb on - strand, within Pf6N2E2_1546 at 1806.071 kb on + strand, within Pf6N2E2_1546 at 1806.071 kb on + strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.239 kb on + strand, within Pf6N2E2_1546 at 1806.240 kb on - strand, within Pf6N2E2_1546 at 1806.240 kb on - strand, within Pf6N2E2_1546 at 1806.323 kb on + strand at 1806.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 1,802,593 - -0.3 1,802,607 + +0.1 1,802,986 + Pf6N2E2_1544 0.41 -4.6 1,802,986 + Pf6N2E2_1544 0.41 +0.6 1,802,986 + Pf6N2E2_1544 0.41 +0.6 1,802,987 - Pf6N2E2_1544 0.41 +1.7 1,802,987 - Pf6N2E2_1544 0.41 -2.5 1,802,987 - Pf6N2E2_1544 0.41 +0.9 1,802,987 - Pf6N2E2_1544 0.41 -0.1 1,802,987 - Pf6N2E2_1544 0.41 -0.3 1,803,121 + Pf6N2E2_1544 0.61 +0.3 1,803,121 + Pf6N2E2_1544 0.61 -0.7 1,803,121 + Pf6N2E2_1544 0.61 -0.0 1,803,422 + +0.2 1,803,423 - -2.9 1,803,423 - -3.6 1,803,439 - -0.6 1,803,523 + -2.3 1,803,523 + +0.9 1,803,718 + +0.0 1,803,872 - Pf6N2E2_1545 0.16 +1.0 1,803,889 + Pf6N2E2_1545 0.17 -0.1 1,803,889 + Pf6N2E2_1545 0.17 +0.3 1,803,889 + Pf6N2E2_1545 0.17 -2.4 1,803,890 - Pf6N2E2_1545 0.17 -1.7 1,804,087 + Pf6N2E2_1545 0.29 -2.7 1,804,228 - Pf6N2E2_1545 0.37 -0.2 1,804,498 + Pf6N2E2_1545 0.52 -2.3 1,804,698 + Pf6N2E2_1545 0.63 -2.0 1,804,698 + Pf6N2E2_1545 0.63 -1.4 1,804,699 - Pf6N2E2_1545 0.64 -1.6 1,804,703 + Pf6N2E2_1545 0.64 +0.6 1,804,907 - Pf6N2E2_1545 0.75 -2.9 1,804,907 - Pf6N2E2_1545 0.75 +0.4 1,805,169 + -1.2 1,805,243 - +1.8 1,805,504 - -0.4 1,805,515 + -0.4 1,805,516 - -0.5 1,805,955 + Pf6N2E2_1546 0.53 -0.5 1,805,956 - Pf6N2E2_1546 0.53 -0.7 1,806,049 + Pf6N2E2_1546 0.64 -2.7 1,806,050 - Pf6N2E2_1546 0.64 +0.1 1,806,071 + Pf6N2E2_1546 0.67 -0.0 1,806,071 + Pf6N2E2_1546 0.67 +1.9 1,806,072 - Pf6N2E2_1546 0.67 -0.5 1,806,072 - Pf6N2E2_1546 0.67 +1.5 1,806,072 - Pf6N2E2_1546 0.67 -0.6 1,806,072 - Pf6N2E2_1546 0.67 +0.9 1,806,072 - Pf6N2E2_1546 0.67 -0.6 1,806,239 + Pf6N2E2_1546 0.86 +0.9 1,806,240 - Pf6N2E2_1546 0.86 -2.4 1,806,240 - Pf6N2E2_1546 0.86 -1.1 1,806,323 + -0.1 1,806,324 - -3.0
Or see this region's nucleotide sequence