Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1440 and Pf6N2E2_1441 are separated by 15 nucleotides Pf6N2E2_1441 and Pf6N2E2_1442 are separated by 120 nucleotides
Pf6N2E2_1440 - Putative tRNA-m1A22 methylase, at 1,690,418 to 1,691,113
_1440
Pf6N2E2_1441 - Uncharacterized oxidoreductase ydgJ (EC 1.-.-.-), at 1,691,129 to 1,692,055
_1441
Pf6N2E2_1442 - Transcriptional regulators of sugar metabolism, at 1,692,176 to 1,692,970
_1442
Position (kb)
1691
1692
1693 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1690.227 kb on + strand at 1690.228 kb on - strand at 1690.277 kb on - strand at 1690.277 kb on - strand at 1690.330 kb on - strand at 1690.330 kb on - strand at 1690.340 kb on + strand at 1690.351 kb on - strand at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.614 kb on + strand, within Pf6N2E2_1440 at 1690.615 kb on - strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440 at 1691.020 kb on - strand, within Pf6N2E2_1440 at 1691.065 kb on + strand at 1691.066 kb on - strand at 1691.066 kb on - strand at 1691.066 kb on - strand at 1691.137 kb on - strand at 1691.251 kb on - strand, within Pf6N2E2_1441 at 1691.354 kb on + strand, within Pf6N2E2_1441 at 1691.354 kb on + strand, within Pf6N2E2_1441 at 1691.355 kb on - strand, within Pf6N2E2_1441 at 1692.079 kb on - strand at 1692.101 kb on - strand at 1692.139 kb on + strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.170 kb on - strand at 1692.182 kb on - strand at 1692.182 kb on - strand at 1692.182 kb on - strand at 1692.228 kb on + strand at 1692.228 kb on + strand at 1692.462 kb on + strand, within Pf6N2E2_1442 at 1692.463 kb on - strand, within Pf6N2E2_1442 at 1692.749 kb on + strand, within Pf6N2E2_1442 at 1692.750 kb on - strand, within Pf6N2E2_1442 at 1692.750 kb on - strand, within Pf6N2E2_1442 at 1692.750 kb on - strand, within Pf6N2E2_1442 at 1692.783 kb on - strand, within Pf6N2E2_1442 at 1692.795 kb on + strand, within Pf6N2E2_1442 at 1692.809 kb on + strand, within Pf6N2E2_1442 at 1693.029 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 1,690,227 + +0.7 1,690,228 - +0.6 1,690,277 - +0.8 1,690,277 - +1.6 1,690,330 - +0.0 1,690,330 - +1.1 1,690,340 + -1.5 1,690,351 - +0.3 1,690,596 + Pf6N2E2_1440 0.26 +0.4 1,690,596 + Pf6N2E2_1440 0.26 +0.7 1,690,614 + Pf6N2E2_1440 0.28 -0.7 1,690,615 - Pf6N2E2_1440 0.28 -2.7 1,690,751 + Pf6N2E2_1440 0.48 +0.5 1,690,751 + Pf6N2E2_1440 0.48 +1.8 1,690,752 - Pf6N2E2_1440 0.48 -1.3 1,690,752 - Pf6N2E2_1440 0.48 -0.5 1,691,020 - Pf6N2E2_1440 0.86 +0.9 1,691,065 + +0.2 1,691,066 - +0.8 1,691,066 - -1.0 1,691,066 - +0.2 1,691,137 - -1.4 1,691,251 - Pf6N2E2_1441 0.13 -0.7 1,691,354 + Pf6N2E2_1441 0.24 -0.9 1,691,354 + Pf6N2E2_1441 0.24 -2.3 1,691,355 - Pf6N2E2_1441 0.24 -0.2 1,692,079 - +1.5 1,692,101 - +1.6 1,692,139 + -0.2 1,692,140 - -0.7 1,692,140 - -0.6 1,692,140 - -0.5 1,692,140 - -1.7 1,692,140 - -0.7 1,692,140 - -0.4 1,692,170 - +0.7 1,692,182 - -1.7 1,692,182 - -0.5 1,692,182 - +0.5 1,692,228 + +0.3 1,692,228 + +0.6 1,692,462 + Pf6N2E2_1442 0.36 -2.0 1,692,463 - Pf6N2E2_1442 0.36 +0.2 1,692,749 + Pf6N2E2_1442 0.72 -0.9 1,692,750 - Pf6N2E2_1442 0.72 +1.1 1,692,750 - Pf6N2E2_1442 0.72 +1.7 1,692,750 - Pf6N2E2_1442 0.72 -0.3 1,692,783 - Pf6N2E2_1442 0.76 +0.3 1,692,795 + Pf6N2E2_1442 0.78 -1.4 1,692,809 + Pf6N2E2_1442 0.80 +0.9 1,693,029 - +0.1
Or see this region's nucleotide sequence