Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1380 and Pf6N2E2_1381 are separated by 458 nucleotides Pf6N2E2_1381 and Pf6N2E2_1382 are separated by 305 nucleotides
Pf6N2E2_1380 - Alcohol dehydrogenase (EC 1.1.1.1); Acetaldehyde dehydrogenase (EC 1.2.1.10), at 1,618,930 to 1,620,108
_1380
Pf6N2E2_1381 - Aldehyde dehydrogenase (EC 1.2.1.3), at 1,620,567 to 1,622,087
_1381
Pf6N2E2_1382 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 1,622,393 to 1,624,168
_1382
Position (kb)
1620
1621
1622
1623 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1620.097 kb on + strand at 1620.097 kb on + strand at 1620.098 kb on - strand at 1620.098 kb on - strand at 1620.113 kb on - strand at 1620.149 kb on - strand at 1620.236 kb on + strand at 1620.236 kb on + strand at 1620.236 kb on + strand at 1620.258 kb on + strand at 1620.259 kb on - strand at 1620.259 kb on - strand at 1620.259 kb on - strand at 1620.282 kb on + strand at 1620.334 kb on + strand at 1620.334 kb on + strand at 1620.365 kb on - strand at 1620.365 kb on - strand at 1620.365 kb on - strand at 1620.395 kb on + strand at 1620.396 kb on - strand at 1620.398 kb on + strand at 1620.398 kb on + strand at 1620.399 kb on - strand at 1620.493 kb on - strand at 1620.595 kb on + strand at 1620.596 kb on - strand at 1620.644 kb on + strand at 1620.644 kb on + strand at 1620.677 kb on - strand at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.940 kb on + strand, within Pf6N2E2_1381 at 1620.941 kb on - strand, within Pf6N2E2_1381 at 1621.024 kb on + strand, within Pf6N2E2_1381 at 1621.025 kb on - strand, within Pf6N2E2_1381 at 1621.025 kb on - strand, within Pf6N2E2_1381 at 1621.604 kb on + strand, within Pf6N2E2_1381 at 1621.604 kb on + strand, within Pf6N2E2_1381 at 1621.708 kb on + strand, within Pf6N2E2_1381 at 1621.984 kb on + strand at 1621.984 kb on + strand at 1621.985 kb on - strand at 1622.080 kb on + strand at 1622.080 kb on + strand at 1622.141 kb on + strand at 1622.141 kb on + strand at 1622.142 kb on - strand at 1622.142 kb on - strand at 1622.192 kb on + strand at 1622.246 kb on - strand at 1622.529 kb on + strand at 1622.529 kb on + strand at 1622.529 kb on + strand at 1622.530 kb on - strand at 1622.704 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.766 kb on + strand, within Pf6N2E2_1382 at 1622.766 kb on + strand, within Pf6N2E2_1382 at 1622.766 kb on + strand at 1622.766 kb on + strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.826 kb on + strand, within Pf6N2E2_1382 at 1622.877 kb on + strand, within Pf6N2E2_1382 at 1622.877 kb on + strand, within Pf6N2E2_1382 at 1622.878 kb on - strand, within Pf6N2E2_1382 at 1622.878 kb on - strand, within Pf6N2E2_1382 at 1623.006 kb on + strand, within Pf6N2E2_1382 at 1623.006 kb on + strand, within Pf6N2E2_1382 at 1623.007 kb on - strand, within Pf6N2E2_1382
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 1,620,097 + -2.2 1,620,097 + +0.0 1,620,098 - -0.1 1,620,098 - +1.3 1,620,113 - +1.2 1,620,149 - +0.1 1,620,236 + +1.0 1,620,236 + -2.4 1,620,236 + +0.6 1,620,258 + -1.6 1,620,259 - -0.7 1,620,259 - -0.4 1,620,259 - -0.7 1,620,282 + +1.6 1,620,334 + +0.1 1,620,334 + -0.7 1,620,365 - -1.1 1,620,365 - +1.7 1,620,365 - +1.5 1,620,395 + -0.3 1,620,396 - +0.4 1,620,398 + -2.5 1,620,398 + +1.6 1,620,399 - -0.7 1,620,493 - +0.3 1,620,595 + -0.7 1,620,596 - -2.9 1,620,644 + -0.1 1,620,644 + -0.9 1,620,677 - -1.7 1,620,937 + Pf6N2E2_1381 0.24 -1.1 1,620,937 + Pf6N2E2_1381 0.24 +1.1 1,620,937 + Pf6N2E2_1381 0.24 -0.9 1,620,937 + Pf6N2E2_1381 0.24 -0.7 1,620,937 + Pf6N2E2_1381 0.24 -1.8 1,620,937 + Pf6N2E2_1381 0.24 -3.2 1,620,937 + -1.7 1,620,938 - Pf6N2E2_1381 0.24 -0.2 1,620,938 - Pf6N2E2_1381 0.24 -1.2 1,620,938 - Pf6N2E2_1381 0.24 -3.5 1,620,938 - Pf6N2E2_1381 0.24 -0.9 1,620,938 - Pf6N2E2_1381 0.24 -2.1 1,620,938 - Pf6N2E2_1381 0.24 -0.4 1,620,940 + Pf6N2E2_1381 0.25 -0.6 1,620,941 - Pf6N2E2_1381 0.25 -0.9 1,621,024 + Pf6N2E2_1381 0.30 -2.9 1,621,025 - Pf6N2E2_1381 0.30 -2.4 1,621,025 - Pf6N2E2_1381 0.30 -2.1 1,621,604 + Pf6N2E2_1381 0.68 -3.7 1,621,604 + Pf6N2E2_1381 0.68 -2.9 1,621,708 + Pf6N2E2_1381 0.75 -1.5 1,621,984 + -3.5 1,621,984 + -2.7 1,621,985 - -2.7 1,622,080 + -0.4 1,622,080 + -3.5 1,622,141 + -1.5 1,622,141 + -3.0 1,622,142 - -1.8 1,622,142 - +1.3 1,622,192 + -4.4 1,622,246 - -1.2 1,622,529 + +0.5 1,622,529 + -2.9 1,622,529 + -0.6 1,622,530 - -1.7 1,622,704 - Pf6N2E2_1382 0.18 -4.5 1,622,765 - Pf6N2E2_1382 0.21 -0.8 1,622,765 - Pf6N2E2_1382 0.21 -2.1 1,622,765 - Pf6N2E2_1382 0.21 -3.2 1,622,765 - Pf6N2E2_1382 0.21 -0.7 1,622,766 + Pf6N2E2_1382 0.21 -0.5 1,622,766 + Pf6N2E2_1382 0.21 +0.5 1,622,766 + -0.7 1,622,766 + Pf6N2E2_1382 0.21 +0.8 1,622,767 - Pf6N2E2_1382 0.21 -0.7 1,622,767 - Pf6N2E2_1382 0.21 +0.3 1,622,767 - Pf6N2E2_1382 0.21 -2.8 1,622,767 - Pf6N2E2_1382 0.21 -0.1 1,622,767 - Pf6N2E2_1382 0.21 +0.2 1,622,826 + Pf6N2E2_1382 0.24 -1.9 1,622,877 + Pf6N2E2_1382 0.27 -1.7 1,622,877 + Pf6N2E2_1382 0.27 -0.7 1,622,878 - Pf6N2E2_1382 0.27 -0.8 1,622,878 - Pf6N2E2_1382 0.27 +1.3 1,623,006 + Pf6N2E2_1382 0.35 -0.3 1,623,006 + Pf6N2E2_1382 0.35 -2.9 1,623,007 - Pf6N2E2_1382 0.35 -0.1
Or see this region's nucleotide sequence