Experiment: Cytidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3262 and Pf6N2E2_3263 are separated by 0 nucleotides Pf6N2E2_3263 and Pf6N2E2_3264 are separated by 7 nucleotides Pf6N2E2_3264 and Pf6N2E2_3265 overlap by 14 nucleotides Pf6N2E2_3265 and Pf6N2E2_3266 overlap by 1 nucleotides
Pf6N2E2_3262 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 3,769,774 to 3,770,748
_3262
Pf6N2E2_3263 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45), at 3,770,749 to 3,771,273
_3263
Pf6N2E2_3264 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 3,771,281 to 3,771,850
_3264
Pf6N2E2_3265 - LptA, protein essential for LPS transport across the periplasm, at 3,771,837 to 3,772,388
_3265
Pf6N2E2_3266 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 3,772,388 to 3,773,113
_3266
Position (kb) 3771
3772 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3770.746 kb on + strand at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.825 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine (N) remove 3,770,746 + +1.7 3,771,716 + Pf6N2E2_3264 0.76 -2.2 3,771,716 + Pf6N2E2_3264 0.76 -0.6 3,771,716 + Pf6N2E2_3264 0.76 -2.3 3,771,716 + Pf6N2E2_3264 0.76 -3.0 3,771,825 + +2.7
Or see this region's nucleotide sequence