Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3181 and Pf6N2E2_3182 are separated by 28 nucleotides Pf6N2E2_3182 and Pf6N2E2_3183 are separated by 88 nucleotides Pf6N2E2_3183 and Pf6N2E2_3184 are separated by 13 nucleotides Pf6N2E2_3184 and Pf6N2E2_3185 are separated by 3 nucleotides
Pf6N2E2_3181 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 3,677,355 to 3,678,503
_3181
Pf6N2E2_3182 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 3,678,532 to 3,678,957
_3182
Pf6N2E2_3183 - Believed to be involved in assembly of Fe-S clusters, at 3,679,046 to 3,679,246
_3183
Pf6N2E2_3184 - Ferredoxin, 2Fe-2S, at 3,679,260 to 3,679,601
_3184
Pf6N2E2_3185 - Chaperone protein HscA, at 3,679,605 to 3,681,467
_3185
Position (kb)
3679
3680 Strain fitness (log2 ratio)
-2
-1
0
1 at 3678.522 kb on - strand at 3678.522 kb on - strand at 3678.522 kb on - strand at 3678.522 kb on - strand at 3678.545 kb on - strand at 3678.553 kb on - strand at 3679.006 kb on - strand at 3679.006 kb on - strand at 3679.008 kb on - strand at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.239 kb on + strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 3,678,522 - +0.9 3,678,522 - +0.2 3,678,522 - -0.4 3,678,522 - -0.5 3,678,545 - -0.6 3,678,553 - +1.5 3,679,006 - -0.3 3,679,006 - -0.6 3,679,008 - -0.4 3,679,224 - Pf6N2E2_3183 0.89 -0.8 3,679,224 - Pf6N2E2_3183 0.89 -1.8 3,679,224 - Pf6N2E2_3183 0.89 -0.7 3,679,224 - Pf6N2E2_3183 0.89 -0.6 3,679,239 + -1.7 3,679,240 - -0.9 3,679,240 - -1.2 3,679,240 - -1.3 3,679,240 - -0.2 3,679,240 - -1.0 3,679,240 - -0.7 3,679,240 - -1.1
Or see this region's nucleotide sequence