Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_201 and Pf6N2E2_202 overlap by 4 nucleotides Pf6N2E2_202 and Pf6N2E2_203 overlap by 11 nucleotides
Pf6N2E2_201 - UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14), at 251,958 to 253,067
_201
Pf6N2E2_202 - 4Fe-4S ferredoxin, iron-sulfur binding, at 253,064 to 254,650
_202
Pf6N2E2_203 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 254,640 to 255,920
_203
Position (kb) 253
254
255 Strain fitness (log2 ratio) -2
-1
0
1
2 at 252.241 kb on + strand, within Pf6N2E2_201 at 252.242 kb on - strand, within Pf6N2E2_201 at 252.242 kb on - strand, within Pf6N2E2_201 at 252.242 kb on - strand, within Pf6N2E2_201 at 252.274 kb on + strand, within Pf6N2E2_201 at 252.274 kb on + strand, within Pf6N2E2_201 at 252.274 kb on + strand, within Pf6N2E2_201 at 252.688 kb on - strand, within Pf6N2E2_201 at 252.688 kb on - strand, within Pf6N2E2_201 at 253.053 kb on + strand at 253.054 kb on - strand at 253.054 kb on - strand at 253.054 kb on - strand at 253.091 kb on + strand at 253.091 kb on + strand at 253.091 kb on + strand at 253.183 kb on + strand at 253.183 kb on + strand at 253.183 kb on + strand at 253.184 kb on - strand at 253.338 kb on + strand, within Pf6N2E2_202 at 253.338 kb on + strand, within Pf6N2E2_202 at 253.339 kb on - strand, within Pf6N2E2_202 at 253.472 kb on - strand, within Pf6N2E2_202 at 253.472 kb on - strand, within Pf6N2E2_202 at 253.472 kb on - strand, within Pf6N2E2_202 at 253.604 kb on - strand, within Pf6N2E2_202 at 253.941 kb on + strand, within Pf6N2E2_202 at 253.941 kb on + strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.158 kb on - strand, within Pf6N2E2_202 at 254.158 kb on - strand, within Pf6N2E2_202 at 254.158 kb on - strand, within Pf6N2E2_202 at 254.218 kb on - strand, within Pf6N2E2_202 at 254.218 kb on - strand, within Pf6N2E2_202 at 254.239 kb on - strand, within Pf6N2E2_202 at 254.826 kb on + strand, within Pf6N2E2_203 at 254.960 kb on + strand, within Pf6N2E2_203 at 255.029 kb on - strand, within Pf6N2E2_203 at 255.232 kb on - strand, within Pf6N2E2_203 at 255.384 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.417 kb on - strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 252,241 + Pf6N2E2_201 0.25 -0.1 252,242 - Pf6N2E2_201 0.26 -1.3 252,242 - Pf6N2E2_201 0.26 +0.2 252,242 - Pf6N2E2_201 0.26 -0.2 252,274 + Pf6N2E2_201 0.28 +1.1 252,274 + Pf6N2E2_201 0.28 -0.5 252,274 + Pf6N2E2_201 0.28 -0.1 252,688 - Pf6N2E2_201 0.66 -0.6 252,688 - Pf6N2E2_201 0.66 +0.5 253,053 + +0.6 253,054 - +0.6 253,054 - +0.7 253,054 - +1.1 253,091 + -1.7 253,091 + -0.2 253,091 + -0.4 253,183 + +0.2 253,183 + +2.3 253,183 + -1.3 253,184 - -1.3 253,338 + Pf6N2E2_202 0.17 +2.1 253,338 + Pf6N2E2_202 0.17 +0.4 253,339 - Pf6N2E2_202 0.17 -1.6 253,472 - Pf6N2E2_202 0.26 -2.6 253,472 - Pf6N2E2_202 0.26 -0.3 253,472 - Pf6N2E2_202 0.26 -0.5 253,604 - Pf6N2E2_202 0.34 -1.3 253,941 + Pf6N2E2_202 0.55 -1.1 253,941 + Pf6N2E2_202 0.55 -1.2 253,942 - Pf6N2E2_202 0.55 +0.6 253,942 - Pf6N2E2_202 0.55 -1.9 253,942 - Pf6N2E2_202 0.55 -0.9 253,942 - Pf6N2E2_202 0.55 +0.1 253,942 - Pf6N2E2_202 0.55 +0.3 253,942 - Pf6N2E2_202 0.55 -1.4 253,942 - Pf6N2E2_202 0.55 +0.5 253,942 - Pf6N2E2_202 0.55 -0.5 253,942 - Pf6N2E2_202 0.55 +0.3 253,942 - Pf6N2E2_202 0.55 +0.6 254,157 + Pf6N2E2_202 0.69 +0.1 254,157 + Pf6N2E2_202 0.69 -0.3 254,157 + Pf6N2E2_202 0.69 +0.5 254,157 + Pf6N2E2_202 0.69 -0.6 254,158 - Pf6N2E2_202 0.69 -0.4 254,158 - Pf6N2E2_202 0.69 -0.9 254,158 - Pf6N2E2_202 0.69 -0.3 254,218 - Pf6N2E2_202 0.73 -0.4 254,218 - Pf6N2E2_202 0.73 +0.6 254,239 - Pf6N2E2_202 0.74 +0.7 254,826 + Pf6N2E2_203 0.15 -0.9 254,960 + Pf6N2E2_203 0.25 -0.5 255,029 - Pf6N2E2_203 0.30 +0.9 255,232 - Pf6N2E2_203 0.46 +0.7 255,384 + Pf6N2E2_203 0.58 -0.8 255,416 + Pf6N2E2_203 0.61 +0.7 255,416 + Pf6N2E2_203 0.61 +0.8 255,416 + Pf6N2E2_203 0.61 +0.1 255,416 + Pf6N2E2_203 0.61 +0.6 255,416 + Pf6N2E2_203 0.61 -0.9 255,416 + Pf6N2E2_203 0.61 -0.5 255,417 - Pf6N2E2_203 0.61 +1.1 255,514 + Pf6N2E2_203 0.68 -0.2 255,514 + Pf6N2E2_203 0.68 -0.4 255,514 + Pf6N2E2_203 0.68 +0.4 255,515 - Pf6N2E2_203 0.68 +1.6 255,515 - Pf6N2E2_203 0.68 +0.4 255,515 - Pf6N2E2_203 0.68 -1.2
Or see this region's nucleotide sequence