Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5565 and Pf6N2E2_5566 overlap by 4 nucleotides Pf6N2E2_5566 and Pf6N2E2_5567 are separated by 212 nucleotides Pf6N2E2_5567 and Pf6N2E2_5568 overlap by 4 nucleotides
Pf6N2E2_5565 - Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake, at 6,382,692 to 6,384,020
_5565
Pf6N2E2_5566 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system (EC 2.7.13.3), at 6,384,017 to 6,385,921
_5566
Pf6N2E2_5567 - Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4), at 6,386,134 to 6,386,868
_5567
Pf6N2E2_5568 - Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4), at 6,386,865 to 6,387,536
_5568
Position (kb)
6384
6385
6386 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.221 kb on - strand, within Pf6N2E2_5565 at 6383.221 kb on - strand, within Pf6N2E2_5565 at 6383.352 kb on - strand, within Pf6N2E2_5565 at 6383.722 kb on + strand, within Pf6N2E2_5565 at 6383.722 kb on + strand, within Pf6N2E2_5565 at 6383.722 kb on + strand, within Pf6N2E2_5565 at 6383.722 kb on + strand, within Pf6N2E2_5565 at 6383.723 kb on - strand, within Pf6N2E2_5565 at 6383.723 kb on - strand, within Pf6N2E2_5565 at 6383.882 kb on + strand, within Pf6N2E2_5565 at 6384.000 kb on + strand at 6384.000 kb on + strand at 6384.000 kb on + strand at 6384.000 kb on + strand at 6384.000 kb on + strand at 6384.001 kb on - strand at 6384.001 kb on - strand at 6384.035 kb on + strand at 6384.044 kb on + strand at 6384.045 kb on - strand at 6384.054 kb on - strand at 6384.259 kb on - strand, within Pf6N2E2_5566 at 6384.259 kb on - strand, within Pf6N2E2_5566 at 6385.024 kb on + strand, within Pf6N2E2_5566 at 6385.024 kb on + strand, within Pf6N2E2_5566 at 6385.025 kb on - strand, within Pf6N2E2_5566 at 6385.025 kb on - strand, within Pf6N2E2_5566 at 6385.053 kb on + strand, within Pf6N2E2_5566 at 6385.053 kb on + strand, within Pf6N2E2_5566 at 6385.053 kb on + strand, within Pf6N2E2_5566 at 6385.054 kb on - strand, within Pf6N2E2_5566 at 6385.054 kb on - strand, within Pf6N2E2_5566 at 6385.054 kb on - strand, within Pf6N2E2_5566 at 6385.054 kb on - strand, within Pf6N2E2_5566 at 6385.170 kb on + strand, within Pf6N2E2_5566 at 6385.171 kb on - strand, within Pf6N2E2_5566 at 6385.173 kb on + strand, within Pf6N2E2_5566 at 6385.173 kb on + strand, within Pf6N2E2_5566 at 6385.174 kb on - strand, within Pf6N2E2_5566 at 6385.174 kb on - strand, within Pf6N2E2_5566 at 6385.174 kb on - strand, within Pf6N2E2_5566 at 6385.261 kb on + strand, within Pf6N2E2_5566 at 6385.395 kb on + strand, within Pf6N2E2_5566 at 6385.396 kb on - strand, within Pf6N2E2_5566 at 6385.396 kb on - strand, within Pf6N2E2_5566 at 6385.396 kb on - strand, within Pf6N2E2_5566 at 6385.396 kb on - strand, within Pf6N2E2_5566 at 6385.398 kb on + strand, within Pf6N2E2_5566 at 6385.398 kb on + strand, within Pf6N2E2_5566 at 6385.418 kb on - strand, within Pf6N2E2_5566 at 6385.431 kb on + strand, within Pf6N2E2_5566 at 6385.470 kb on + strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.561 kb on - strand, within Pf6N2E2_5566 at 6385.561 kb on - strand, within Pf6N2E2_5566 at 6385.561 kb on - strand, within Pf6N2E2_5566 at 6385.602 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.690 kb on - strand, within Pf6N2E2_5566 at 6385.690 kb on - strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.696 kb on - strand, within Pf6N2E2_5566 at 6385.696 kb on - strand, within Pf6N2E2_5566 at 6385.696 kb on - strand, within Pf6N2E2_5566 at 6385.696 kb on - strand, within Pf6N2E2_5566 at 6385.769 kb on + strand at 6385.769 kb on + strand at 6385.769 kb on + strand at 6385.769 kb on + strand at 6385.770 kb on - strand at 6385.776 kb on + strand at 6385.777 kb on - strand at 6385.777 kb on - strand at 6386.020 kb on + strand at 6386.037 kb on - strand at 6386.045 kb on + strand at 6386.046 kb on - strand at 6386.136 kb on - strand at 6386.136 kb on - strand at 6386.248 kb on + strand, within Pf6N2E2_5567 at 6386.248 kb on + strand, within Pf6N2E2_5567 at 6386.249 kb on - strand, within Pf6N2E2_5567 at 6386.821 kb on + strand at 6386.838 kb on - strand at 6386.859 kb on + strand at 6386.859 kb on + strand at 6386.868 kb on + strand at 6386.868 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 6,383,110 + Pf6N2E2_5565 0.31 -0.3 6,383,110 + Pf6N2E2_5565 0.31 -0.5 6,383,110 + Pf6N2E2_5565 0.31 -1.7 6,383,110 + Pf6N2E2_5565 0.31 -0.7 6,383,110 + Pf6N2E2_5565 0.31 -1.0 6,383,110 + Pf6N2E2_5565 0.31 -1.4 6,383,110 + Pf6N2E2_5565 0.31 -0.6 6,383,110 + Pf6N2E2_5565 0.31 -0.9 6,383,110 + Pf6N2E2_5565 0.31 -0.3 6,383,111 - Pf6N2E2_5565 0.32 +0.0 6,383,111 - Pf6N2E2_5565 0.32 +1.3 6,383,111 - Pf6N2E2_5565 0.32 +2.2 6,383,111 - Pf6N2E2_5565 0.32 +0.7 6,383,221 - Pf6N2E2_5565 0.40 +0.4 6,383,221 - Pf6N2E2_5565 0.40 -1.0 6,383,352 - Pf6N2E2_5565 0.50 -0.8 6,383,722 + Pf6N2E2_5565 0.78 +0.0 6,383,722 + Pf6N2E2_5565 0.78 -0.7 6,383,722 + Pf6N2E2_5565 0.78 -1.9 6,383,722 + Pf6N2E2_5565 0.78 -1.4 6,383,723 - Pf6N2E2_5565 0.78 +0.2 6,383,723 - Pf6N2E2_5565 0.78 +1.1 6,383,882 + Pf6N2E2_5565 0.90 +0.7 6,384,000 + +0.4 6,384,000 + +0.0 6,384,000 + -0.5 6,384,000 + -3.1 6,384,000 + -0.6 6,384,001 - +0.1 6,384,001 - +0.7 6,384,035 + +1.5 6,384,044 + +1.2 6,384,045 - +1.0 6,384,054 - +0.4 6,384,259 - Pf6N2E2_5566 0.13 +0.4 6,384,259 - Pf6N2E2_5566 0.13 -0.7 6,385,024 + Pf6N2E2_5566 0.53 -1.0 6,385,024 + Pf6N2E2_5566 0.53 -0.4 6,385,025 - Pf6N2E2_5566 0.53 -1.6 6,385,025 - Pf6N2E2_5566 0.53 +0.8 6,385,053 + Pf6N2E2_5566 0.54 -1.6 6,385,053 + Pf6N2E2_5566 0.54 -0.7 6,385,053 + Pf6N2E2_5566 0.54 -1.4 6,385,054 - Pf6N2E2_5566 0.54 -0.8 6,385,054 - Pf6N2E2_5566 0.54 -1.8 6,385,054 - Pf6N2E2_5566 0.54 -0.3 6,385,054 - Pf6N2E2_5566 0.54 -1.0 6,385,170 + Pf6N2E2_5566 0.61 +0.2 6,385,171 - Pf6N2E2_5566 0.61 +0.2 6,385,173 + Pf6N2E2_5566 0.61 -0.5 6,385,173 + Pf6N2E2_5566 0.61 -0.9 6,385,174 - Pf6N2E2_5566 0.61 +0.0 6,385,174 - Pf6N2E2_5566 0.61 -2.9 6,385,174 - Pf6N2E2_5566 0.61 -0.7 6,385,261 + Pf6N2E2_5566 0.65 -2.9 6,385,395 + Pf6N2E2_5566 0.72 -0.7 6,385,396 - Pf6N2E2_5566 0.72 -0.6 6,385,396 - Pf6N2E2_5566 0.72 +0.1 6,385,396 - Pf6N2E2_5566 0.72 -1.3 6,385,396 - Pf6N2E2_5566 0.72 +0.6 6,385,398 + Pf6N2E2_5566 0.72 -0.4 6,385,398 + Pf6N2E2_5566 0.72 -2.1 6,385,418 - Pf6N2E2_5566 0.74 +0.6 6,385,431 + Pf6N2E2_5566 0.74 -0.8 6,385,470 + Pf6N2E2_5566 0.76 +0.3 6,385,501 - Pf6N2E2_5566 0.78 +1.0 6,385,501 - Pf6N2E2_5566 0.78 +0.7 6,385,501 - Pf6N2E2_5566 0.78 +1.1 6,385,501 - Pf6N2E2_5566 0.78 -1.3 6,385,501 - Pf6N2E2_5566 0.78 +0.5 6,385,560 + Pf6N2E2_5566 0.81 +0.6 6,385,560 + Pf6N2E2_5566 0.81 -1.8 6,385,560 + Pf6N2E2_5566 0.81 -0.1 6,385,560 + Pf6N2E2_5566 0.81 +0.6 6,385,560 + Pf6N2E2_5566 0.81 +0.0 6,385,561 - Pf6N2E2_5566 0.81 -0.2 6,385,561 - Pf6N2E2_5566 0.81 -0.8 6,385,561 - Pf6N2E2_5566 0.81 +1.4 6,385,602 + Pf6N2E2_5566 0.83 +0.7 6,385,689 + Pf6N2E2_5566 0.88 +0.1 6,385,689 + Pf6N2E2_5566 0.88 +0.4 6,385,689 + Pf6N2E2_5566 0.88 -2.6 6,385,689 + Pf6N2E2_5566 0.88 -1.6 6,385,689 + Pf6N2E2_5566 0.88 -0.8 6,385,690 - Pf6N2E2_5566 0.88 -0.2 6,385,690 - Pf6N2E2_5566 0.88 -1.7 6,385,695 + Pf6N2E2_5566 0.88 -0.6 6,385,695 + Pf6N2E2_5566 0.88 -0.6 6,385,695 + Pf6N2E2_5566 0.88 +0.4 6,385,695 + Pf6N2E2_5566 0.88 -0.3 6,385,695 + Pf6N2E2_5566 0.88 +0.2 6,385,695 + Pf6N2E2_5566 0.88 -1.4 6,385,695 + Pf6N2E2_5566 0.88 +0.1 6,385,695 + Pf6N2E2_5566 0.88 -1.0 6,385,695 + Pf6N2E2_5566 0.88 -2.3 6,385,695 + Pf6N2E2_5566 0.88 -1.1 6,385,695 + Pf6N2E2_5566 0.88 +0.1 6,385,696 - Pf6N2E2_5566 0.88 +2.0 6,385,696 - Pf6N2E2_5566 0.88 -1.1 6,385,696 - Pf6N2E2_5566 0.88 -0.5 6,385,696 - Pf6N2E2_5566 0.88 -0.2 6,385,769 + -0.9 6,385,769 + +0.3 6,385,769 + -1.6 6,385,769 + -0.4 6,385,770 - -1.0 6,385,776 + -0.6 6,385,777 - +0.1 6,385,777 - +0.4 6,386,020 + -0.3 6,386,037 - +1.2 6,386,045 + +0.7 6,386,046 - -0.1 6,386,136 - +1.5 6,386,136 - -0.1 6,386,248 + Pf6N2E2_5567 0.16 +2.9 6,386,248 + Pf6N2E2_5567 0.16 -1.6 6,386,249 - Pf6N2E2_5567 0.16 +1.5 6,386,821 + +1.3 6,386,838 - -0.7 6,386,859 + +1.4 6,386,859 + +0.2 6,386,868 + -0.1 6,386,868 + -0.3
Or see this region's nucleotide sequence