Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5385 and Pf6N2E2_5386 are separated by 99 nucleotides Pf6N2E2_5386 and Pf6N2E2_5387 are separated by 35 nucleotides
Pf6N2E2_5385 - Alginate biosynthesis protein AlgK precursor, at 6,188,941 to 6,190,341
_5385
Pf6N2E2_5386 - Alginate biosynthesis protein Alg44, at 6,190,441 to 6,191,610
_5386
Pf6N2E2_5387 - Alginate biosynthesis protein Alg8, at 6,191,646 to 6,193,127
_5387
Position (kb)
6190
6191
6192 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.459 kb on - strand, within Pf6N2E2_5385 at 6189.501 kb on - strand, within Pf6N2E2_5385 at 6189.719 kb on + strand, within Pf6N2E2_5385 at 6189.720 kb on - strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.741 kb on - strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385 at 6190.176 kb on - strand, within Pf6N2E2_5385 at 6190.199 kb on + strand, within Pf6N2E2_5385 at 6190.199 kb on + strand, within Pf6N2E2_5385 at 6190.199 kb on + strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.342 kb on - strand at 6190.342 kb on - strand at 6190.342 kb on - strand at 6190.414 kb on + strand at 6190.415 kb on - strand at 6190.416 kb on + strand at 6190.416 kb on + strand at 6190.416 kb on + strand at 6190.431 kb on + strand at 6190.432 kb on - strand at 6190.432 kb on - strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.446 kb on + strand at 6190.446 kb on + strand at 6190.511 kb on - strand at 6190.665 kb on + strand, within Pf6N2E2_5386 at 6191.004 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.067 kb on - strand, within Pf6N2E2_5386 at 6191.345 kb on + strand, within Pf6N2E2_5386 at 6191.346 kb on - strand, within Pf6N2E2_5386 at 6191.417 kb on + strand, within Pf6N2E2_5386 at 6191.418 kb on - strand, within Pf6N2E2_5386 at 6191.506 kb on - strand at 6191.584 kb on + strand at 6191.584 kb on + strand at 6191.584 kb on + strand at 6191.585 kb on - strand at 6191.585 kb on - strand at 6191.585 kb on - strand at 6191.624 kb on + strand at 6191.625 kb on - strand at 6191.628 kb on + strand at 6191.628 kb on + strand at 6191.628 kb on + strand at 6191.629 kb on - strand at 6191.629 kb on - strand at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.807 kb on - strand, within Pf6N2E2_5387 at 6191.807 kb on - strand, within Pf6N2E2_5387 at 6191.807 kb on - strand, within Pf6N2E2_5387 at 6191.807 kb on - strand, within Pf6N2E2_5387 at 6191.839 kb on + strand, within Pf6N2E2_5387 at 6191.840 kb on - strand, within Pf6N2E2_5387 at 6191.840 kb on - strand, within Pf6N2E2_5387 at 6191.842 kb on + strand, within Pf6N2E2_5387 at 6191.935 kb on + strand, within Pf6N2E2_5387 at 6191.935 kb on + strand, within Pf6N2E2_5387 at 6191.936 kb on - strand, within Pf6N2E2_5387 at 6191.936 kb on - strand, within Pf6N2E2_5387 at 6191.959 kb on + strand, within Pf6N2E2_5387 at 6191.959 kb on + strand, within Pf6N2E2_5387 at 6191.959 kb on + strand, within Pf6N2E2_5387 at 6191.960 kb on - strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.197 kb on - strand, within Pf6N2E2_5387 at 6192.454 kb on + strand, within Pf6N2E2_5387 at 6192.455 kb on - strand, within Pf6N2E2_5387 at 6192.580 kb on - strand, within Pf6N2E2_5387
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 6,189,458 + Pf6N2E2_5385 0.37 +0.1 6,189,458 + Pf6N2E2_5385 0.37 +0.1 6,189,459 - Pf6N2E2_5385 0.37 +0.8 6,189,501 - Pf6N2E2_5385 0.40 +1.1 6,189,719 + Pf6N2E2_5385 0.56 -0.4 6,189,720 - Pf6N2E2_5385 0.56 +0.5 6,189,740 + Pf6N2E2_5385 0.57 -0.3 6,189,740 + Pf6N2E2_5385 0.57 +0.5 6,189,740 + Pf6N2E2_5385 0.57 +0.0 6,189,741 - Pf6N2E2_5385 0.57 -1.4 6,189,806 + Pf6N2E2_5385 0.62 +0.1 6,189,806 + Pf6N2E2_5385 0.62 +1.0 6,189,807 - Pf6N2E2_5385 0.62 -2.2 6,189,807 - Pf6N2E2_5385 0.62 -1.2 6,189,807 - Pf6N2E2_5385 0.62 +0.4 6,189,807 - Pf6N2E2_5385 0.62 -0.0 6,189,929 + Pf6N2E2_5385 0.71 -0.5 6,189,929 + Pf6N2E2_5385 0.71 +0.8 6,189,929 + Pf6N2E2_5385 0.71 +0.3 6,189,930 - Pf6N2E2_5385 0.71 -0.8 6,189,930 - Pf6N2E2_5385 0.71 -0.5 6,190,176 - Pf6N2E2_5385 0.88 +0.2 6,190,199 + Pf6N2E2_5385 0.90 +0.1 6,190,199 + Pf6N2E2_5385 0.90 +0.1 6,190,199 + Pf6N2E2_5385 0.90 -0.2 6,190,200 - Pf6N2E2_5385 0.90 +0.2 6,190,200 - Pf6N2E2_5385 0.90 +1.3 6,190,200 - Pf6N2E2_5385 0.90 +0.9 6,190,200 - Pf6N2E2_5385 0.90 -1.6 6,190,200 - Pf6N2E2_5385 0.90 -1.8 6,190,200 - Pf6N2E2_5385 0.90 +0.1 6,190,200 - Pf6N2E2_5385 0.90 +0.8 6,190,200 - Pf6N2E2_5385 0.90 +0.0 6,190,341 + -0.4 6,190,341 + +0.8 6,190,341 + +0.4 6,190,341 + -0.6 6,190,341 + -0.5 6,190,342 - +0.9 6,190,342 - +0.0 6,190,342 - +0.1 6,190,414 + +1.0 6,190,415 - -0.7 6,190,416 + -1.2 6,190,416 + +0.1 6,190,416 + +0.4 6,190,431 + +0.2 6,190,432 - +0.3 6,190,432 - -0.7 6,190,442 + +0.9 6,190,442 + +1.3 6,190,442 + +1.0 6,190,442 + -0.0 6,190,442 + +0.1 6,190,442 + -0.3 6,190,446 + -0.2 6,190,446 + +0.5 6,190,511 - +0.1 6,190,665 + Pf6N2E2_5386 0.19 -0.7 6,191,004 + Pf6N2E2_5386 0.48 -1.3 6,191,057 + Pf6N2E2_5386 0.53 +0.1 6,191,057 + Pf6N2E2_5386 0.53 -1.1 6,191,057 + Pf6N2E2_5386 0.53 +2.1 6,191,057 + Pf6N2E2_5386 0.53 -0.7 6,191,057 + Pf6N2E2_5386 0.53 +0.1 6,191,057 + Pf6N2E2_5386 0.53 +0.5 6,191,057 + Pf6N2E2_5386 0.53 -0.4 6,191,057 + Pf6N2E2_5386 0.53 -0.0 6,191,057 + Pf6N2E2_5386 0.53 -0.3 6,191,058 - Pf6N2E2_5386 0.53 +0.8 6,191,058 - Pf6N2E2_5386 0.53 -1.2 6,191,058 - Pf6N2E2_5386 0.53 +0.6 6,191,058 - Pf6N2E2_5386 0.53 +0.5 6,191,058 - Pf6N2E2_5386 0.53 -1.1 6,191,058 - Pf6N2E2_5386 0.53 +0.1 6,191,067 - Pf6N2E2_5386 0.54 +0.7 6,191,345 + Pf6N2E2_5386 0.77 -0.0 6,191,346 - Pf6N2E2_5386 0.77 +1.1 6,191,417 + Pf6N2E2_5386 0.83 -0.8 6,191,418 - Pf6N2E2_5386 0.84 -0.0 6,191,506 - +0.9 6,191,584 + -0.3 6,191,584 + +0.8 6,191,584 + +0.9 6,191,585 - +0.3 6,191,585 - -0.5 6,191,585 - +0.3 6,191,624 + +0.8 6,191,625 - +1.0 6,191,628 + -1.0 6,191,628 + -1.3 6,191,628 + +0.7 6,191,629 - -0.6 6,191,629 - +0.6 6,191,806 + Pf6N2E2_5387 0.11 -0.1 6,191,806 + Pf6N2E2_5387 0.11 +0.3 6,191,806 + Pf6N2E2_5387 0.11 +0.1 6,191,806 + Pf6N2E2_5387 0.11 +0.9 6,191,806 + Pf6N2E2_5387 0.11 +0.0 6,191,806 + Pf6N2E2_5387 0.11 +0.2 6,191,806 + Pf6N2E2_5387 0.11 -0.2 6,191,806 + Pf6N2E2_5387 0.11 +1.6 6,191,806 + Pf6N2E2_5387 0.11 +0.3 6,191,806 + Pf6N2E2_5387 0.11 +0.9 6,191,807 - Pf6N2E2_5387 0.11 +1.4 6,191,807 - Pf6N2E2_5387 0.11 +0.5 6,191,807 - Pf6N2E2_5387 0.11 +0.5 6,191,807 - Pf6N2E2_5387 0.11 -2.7 6,191,839 + Pf6N2E2_5387 0.13 -0.2 6,191,840 - Pf6N2E2_5387 0.13 +2.6 6,191,840 - Pf6N2E2_5387 0.13 +0.6 6,191,842 + Pf6N2E2_5387 0.13 -1.3 6,191,935 + Pf6N2E2_5387 0.20 +0.2 6,191,935 + Pf6N2E2_5387 0.20 -1.4 6,191,936 - Pf6N2E2_5387 0.20 -0.5 6,191,936 - Pf6N2E2_5387 0.20 +0.7 6,191,959 + Pf6N2E2_5387 0.21 +0.0 6,191,959 + Pf6N2E2_5387 0.21 -1.7 6,191,959 + Pf6N2E2_5387 0.21 -1.2 6,191,960 - Pf6N2E2_5387 0.21 +1.4 6,192,196 + Pf6N2E2_5387 0.37 -1.4 6,192,196 + Pf6N2E2_5387 0.37 -0.1 6,192,196 + Pf6N2E2_5387 0.37 +1.3 6,192,196 + Pf6N2E2_5387 0.37 +0.4 6,192,196 + Pf6N2E2_5387 0.37 -0.4 6,192,196 + Pf6N2E2_5387 0.37 +0.8 6,192,196 + Pf6N2E2_5387 0.37 +0.3 6,192,196 + Pf6N2E2_5387 0.37 -0.3 6,192,196 + Pf6N2E2_5387 0.37 +0.0 6,192,196 + Pf6N2E2_5387 0.37 +0.8 6,192,196 + Pf6N2E2_5387 0.37 -2.2 6,192,196 + Pf6N2E2_5387 0.37 +0.3 6,192,197 - Pf6N2E2_5387 0.37 +0.3 6,192,454 + Pf6N2E2_5387 0.55 -0.5 6,192,455 - Pf6N2E2_5387 0.55 +1.1 6,192,580 - Pf6N2E2_5387 0.63 +1.7
Or see this region's nucleotide sequence