Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_467 and Pf6N2E2_468 are separated by 6 nucleotides Pf6N2E2_468 and Pf6N2E2_469 are separated by 251 nucleotides Pf6N2E2_469 and Pf6N2E2_470 overlap by 4 nucleotides Pf6N2E2_470 and Pf6N2E2_471 overlap by 1 nucleotides
Pf6N2E2_467 - Sensory box/GGDEF family protein, at 532,363 to 534,441
_467
Pf6N2E2_468 - DNA alkylation repair enzyme, at 534,448 to 535,551
_468
Pf6N2E2_469 - lipoprotein, putative, at 535,803 to 536,111
_469
Pf6N2E2_470 - hypothetical protein, at 536,108 to 536,431
_470
Pf6N2E2_471 - putative metalloprotease, at 536,431 to 537,492
_471
Position (kb) 534
535
536 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 533.716 kb on + strand, within Pf6N2E2_467 at 533.717 kb on - strand, within Pf6N2E2_467 at 533.719 kb on + strand, within Pf6N2E2_467 at 533.905 kb on + strand, within Pf6N2E2_467 at 533.905 kb on + strand, within Pf6N2E2_467 at 533.905 kb on + strand, within Pf6N2E2_467 at 533.906 kb on - strand, within Pf6N2E2_467 at 533.906 kb on - strand, within Pf6N2E2_467 at 533.906 kb on - strand, within Pf6N2E2_467 at 533.919 kb on + strand, within Pf6N2E2_467 at 533.919 kb on + strand, within Pf6N2E2_467 at 533.920 kb on - strand, within Pf6N2E2_467 at 534.160 kb on + strand, within Pf6N2E2_467 at 534.160 kb on + strand, within Pf6N2E2_467 at 534.160 kb on + strand, within Pf6N2E2_467 at 534.161 kb on - strand, within Pf6N2E2_467 at 534.651 kb on - strand, within Pf6N2E2_468 at 534.971 kb on + strand, within Pf6N2E2_468 at 534.971 kb on + strand, within Pf6N2E2_468 at 534.972 kb on - strand, within Pf6N2E2_468 at 534.972 kb on - strand, within Pf6N2E2_468 at 534.972 kb on - strand, within Pf6N2E2_468 at 534.972 kb on - strand, within Pf6N2E2_468 at 534.972 kb on - strand, within Pf6N2E2_468 at 535.096 kb on + strand, within Pf6N2E2_468 at 535.208 kb on + strand, within Pf6N2E2_468 at 535.209 kb on - strand, within Pf6N2E2_468 at 535.251 kb on - strand, within Pf6N2E2_468 at 535.251 kb on - strand, within Pf6N2E2_468 at 535.262 kb on + strand, within Pf6N2E2_468 at 535.262 kb on + strand, within Pf6N2E2_468 at 535.262 kb on + strand, within Pf6N2E2_468 at 535.262 kb on + strand, within Pf6N2E2_468 at 535.262 kb on + strand, within Pf6N2E2_468 at 535.263 kb on - strand, within Pf6N2E2_468 at 535.263 kb on - strand, within Pf6N2E2_468 at 535.263 kb on - strand, within Pf6N2E2_468 at 535.263 kb on - strand, within Pf6N2E2_468 at 535.354 kb on + strand, within Pf6N2E2_468 at 535.460 kb on + strand at 535.581 kb on + strand at 535.582 kb on - strand at 535.582 kb on - strand at 535.582 kb on - strand at 535.582 kb on - strand at 535.584 kb on - strand at 535.584 kb on - strand at 535.740 kb on - strand at 536.052 kb on + strand, within Pf6N2E2_469 at 536.052 kb on + strand, within Pf6N2E2_469 at 536.053 kb on - strand, within Pf6N2E2_469 at 536.110 kb on - strand at 536.110 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 533,716 + Pf6N2E2_467 0.65 +0.2 533,717 - Pf6N2E2_467 0.65 -0.4 533,719 + Pf6N2E2_467 0.65 +1.6 533,905 + Pf6N2E2_467 0.74 +1.4 533,905 + Pf6N2E2_467 0.74 +0.3 533,905 + Pf6N2E2_467 0.74 -0.8 533,906 - Pf6N2E2_467 0.74 -0.7 533,906 - Pf6N2E2_467 0.74 -0.4 533,906 - Pf6N2E2_467 0.74 -0.4 533,919 + Pf6N2E2_467 0.75 -1.6 533,919 + Pf6N2E2_467 0.75 -0.4 533,920 - Pf6N2E2_467 0.75 +1.0 534,160 + Pf6N2E2_467 0.86 +1.0 534,160 + Pf6N2E2_467 0.86 +0.7 534,160 + Pf6N2E2_467 0.86 -0.0 534,161 - Pf6N2E2_467 0.86 +0.2 534,651 - Pf6N2E2_468 0.18 -0.6 534,971 + Pf6N2E2_468 0.47 +0.9 534,971 + Pf6N2E2_468 0.47 +1.2 534,972 - Pf6N2E2_468 0.47 -1.1 534,972 - Pf6N2E2_468 0.47 +0.4 534,972 - Pf6N2E2_468 0.47 +1.4 534,972 - Pf6N2E2_468 0.47 +1.2 534,972 - Pf6N2E2_468 0.47 +1.9 535,096 + Pf6N2E2_468 0.59 -0.6 535,208 + Pf6N2E2_468 0.69 +0.7 535,209 - Pf6N2E2_468 0.69 -0.5 535,251 - Pf6N2E2_468 0.73 +1.0 535,251 - Pf6N2E2_468 0.73 -0.3 535,262 + Pf6N2E2_468 0.74 +0.4 535,262 + Pf6N2E2_468 0.74 +1.3 535,262 + Pf6N2E2_468 0.74 -1.1 535,262 + Pf6N2E2_468 0.74 +0.2 535,262 + Pf6N2E2_468 0.74 +0.4 535,263 - Pf6N2E2_468 0.74 -0.4 535,263 - Pf6N2E2_468 0.74 +0.7 535,263 - Pf6N2E2_468 0.74 +1.4 535,263 - Pf6N2E2_468 0.74 -0.1 535,354 + Pf6N2E2_468 0.82 -0.7 535,460 + -3.4 535,581 + +0.4 535,582 - +1.0 535,582 - -1.4 535,582 - +0.6 535,582 - +0.2 535,584 - +1.0 535,584 - -0.0 535,740 - +2.0 536,052 + Pf6N2E2_469 0.81 +1.6 536,052 + Pf6N2E2_469 0.81 -2.1 536,053 - Pf6N2E2_469 0.81 +1.1 536,110 - +0.3 536,110 - -0.6
Or see this region's nucleotide sequence