Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_442 and Pf6N2E2_443 are separated by 0 nucleotides Pf6N2E2_443 and Pf6N2E2_444 are separated by 126 nucleotides Pf6N2E2_444 and Pf6N2E2_445 are separated by 234 nucleotides Pf6N2E2_445 and Pf6N2E2_446 are separated by 200 nucleotides
Pf6N2E2_442 - FIG00962345: hypothetical protein, at 506,038 to 506,268
_442
Pf6N2E2_443 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 506,269 to 506,550
_443
Pf6N2E2_444 - hypothetical protein, at 506,677 to 506,892
_444
Pf6N2E2_445 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 507,127 to 507,966
_445
Pf6N2E2_446 - Glutathione reductase (EC 1.8.1.7), at 508,167 to 509,525
_446
Position (kb) 507
508 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 506.223 kb on + strand, within Pf6N2E2_442 at 506.270 kb on + strand at 506.270 kb on + strand at 506.270 kb on + strand at 506.271 kb on - strand at 506.271 kb on - strand at 506.271 kb on - strand at 506.414 kb on + strand, within Pf6N2E2_443 at 506.483 kb on + strand, within Pf6N2E2_443 at 506.483 kb on + strand, within Pf6N2E2_443 at 506.484 kb on - strand, within Pf6N2E2_443 at 506.526 kb on + strand at 506.526 kb on + strand at 506.527 kb on - strand at 506.591 kb on - strand at 506.591 kb on - strand at 506.591 kb on - strand at 506.615 kb on + strand at 506.615 kb on + strand at 506.616 kb on - strand at 506.659 kb on + strand at 506.659 kb on + strand at 506.659 kb on + strand at 506.659 kb on + strand at 506.659 kb on + strand at 506.660 kb on - strand at 506.660 kb on - strand at 506.660 kb on - strand at 506.662 kb on + strand at 506.662 kb on + strand at 506.662 kb on + strand at 506.662 kb on + strand at 506.679 kb on - strand at 506.819 kb on + strand, within Pf6N2E2_444 at 506.819 kb on + strand, within Pf6N2E2_444 at 506.819 kb on + strand, within Pf6N2E2_444 at 506.819 kb on + strand, within Pf6N2E2_444 at 506.820 kb on - strand, within Pf6N2E2_444 at 506.820 kb on - strand, within Pf6N2E2_444 at 506.820 kb on - strand, within Pf6N2E2_444 at 506.894 kb on + strand at 506.894 kb on + strand at 506.894 kb on + strand at 506.894 kb on + strand at 506.894 kb on + strand at 506.894 kb on + strand at 506.895 kb on - strand at 506.895 kb on - strand at 506.895 kb on - strand at 506.930 kb on - strand at 506.930 kb on - strand at 506.930 kb on - strand at 506.930 kb on - strand at 506.930 kb on - strand at 506.955 kb on + strand at 506.955 kb on + strand at 507.029 kb on + strand at 507.029 kb on + strand at 507.030 kb on - strand at 507.030 kb on - strand at 507.030 kb on - strand at 507.030 kb on - strand at 507.062 kb on + strand at 507.063 kb on - strand at 507.063 kb on - strand at 507.063 kb on - strand at 507.063 kb on - strand at 507.063 kb on - strand at 507.082 kb on + strand at 507.082 kb on + strand at 507.082 kb on + strand at 507.083 kb on - strand at 507.083 kb on - strand at 507.124 kb on + strand at 507.124 kb on + strand at 507.124 kb on + strand at 507.125 kb on - strand at 507.125 kb on - strand at 507.125 kb on - strand at 507.126 kb on + strand at 507.126 kb on + strand at 507.126 kb on + strand at 507.126 kb on + strand at 507.126 kb on + strand at 507.127 kb on - strand at 507.127 kb on - strand at 507.127 kb on - strand at 507.186 kb on + strand at 507.187 kb on - strand at 507.187 kb on - strand at 507.334 kb on + strand, within Pf6N2E2_445 at 507.334 kb on + strand, within Pf6N2E2_445 at 507.335 kb on - strand, within Pf6N2E2_445 at 507.376 kb on + strand, within Pf6N2E2_445 at 507.421 kb on + strand, within Pf6N2E2_445 at 507.548 kb on + strand, within Pf6N2E2_445 at 507.548 kb on + strand, within Pf6N2E2_445 at 507.571 kb on + strand, within Pf6N2E2_445 at 507.571 kb on + strand, within Pf6N2E2_445 at 507.635 kb on - strand, within Pf6N2E2_445 at 507.673 kb on + strand, within Pf6N2E2_445 at 507.673 kb on + strand, within Pf6N2E2_445 at 507.746 kb on - strand, within Pf6N2E2_445 at 507.862 kb on + strand, within Pf6N2E2_445 at 507.862 kb on + strand, within Pf6N2E2_445 at 507.863 kb on - strand, within Pf6N2E2_445 at 507.863 kb on - strand, within Pf6N2E2_445 at 507.907 kb on + strand at 507.908 kb on - strand at 507.908 kb on - strand at 507.943 kb on + strand at 507.943 kb on + strand at 507.943 kb on + strand at 507.943 kb on + strand at 507.944 kb on - strand at 507.944 kb on - strand at 507.944 kb on - strand at 507.961 kb on + strand at 508.137 kb on + strand at 508.138 kb on - strand at 508.138 kb on - strand at 508.138 kb on - strand at 508.138 kb on - strand at 508.138 kb on - strand at 508.162 kb on + strand at 508.162 kb on + strand at 508.162 kb on + strand at 508.163 kb on - strand at 508.163 kb on - strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.189 kb on - strand at 508.189 kb on - strand at 508.189 kb on - strand at 508.189 kb on - strand at 508.189 kb on - strand at 508.217 kb on - strand at 508.329 kb on + strand, within Pf6N2E2_446 at 508.330 kb on - strand, within Pf6N2E2_446 at 508.341 kb on + strand at 508.341 kb on + strand, within Pf6N2E2_446 at 508.371 kb on + strand, within Pf6N2E2_446 at 508.372 kb on - strand, within Pf6N2E2_446 at 508.458 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.938 kb on + strand, within Pf6N2E2_446 at 508.938 kb on + strand, within Pf6N2E2_446 at 508.938 kb on + strand, within Pf6N2E2_446 at 508.939 kb on - strand, within Pf6N2E2_446
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 506,223 + Pf6N2E2_442 0.80 -2.5 506,270 + -2.4 506,270 + -0.4 506,270 + -0.9 506,271 - -0.1 506,271 - +0.6 506,271 - -0.1 506,414 + Pf6N2E2_443 0.51 -1.0 506,483 + Pf6N2E2_443 0.76 -0.5 506,483 + Pf6N2E2_443 0.76 +2.5 506,484 - Pf6N2E2_443 0.76 +0.8 506,526 + +0.1 506,526 + -1.1 506,527 - +0.6 506,591 - +0.6 506,591 - +0.5 506,591 - -0.7 506,615 + -2.4 506,615 + -0.5 506,616 - +0.0 506,659 + -0.2 506,659 + -0.2 506,659 + +0.9 506,659 + -1.4 506,659 + +0.3 506,660 - +1.3 506,660 - -0.7 506,660 - +1.3 506,662 + -0.5 506,662 + +0.1 506,662 + +0.5 506,662 + +0.0 506,679 - +0.2 506,819 + Pf6N2E2_444 0.66 -0.1 506,819 + Pf6N2E2_444 0.66 +0.7 506,819 + Pf6N2E2_444 0.66 -2.5 506,819 + Pf6N2E2_444 0.66 +0.2 506,820 - Pf6N2E2_444 0.66 +0.1 506,820 - Pf6N2E2_444 0.66 +0.8 506,820 - Pf6N2E2_444 0.66 -0.2 506,894 + +1.0 506,894 + -0.8 506,894 + -0.8 506,894 + +0.2 506,894 + +0.5 506,894 + -1.3 506,895 - -0.5 506,895 - +0.8 506,895 - +0.5 506,930 - +0.6 506,930 - +0.6 506,930 - -0.6 506,930 - -0.5 506,930 - +0.8 506,955 + -1.4 506,955 + +0.1 507,029 + +0.9 507,029 + -0.7 507,030 - +0.7 507,030 - +0.9 507,030 - +0.7 507,030 - +1.3 507,062 + -0.3 507,063 - +2.6 507,063 - -0.1 507,063 - +1.3 507,063 - +3.2 507,063 - +2.6 507,082 + +0.7 507,082 + -0.6 507,082 + +0.9 507,083 - +1.7 507,083 - +1.1 507,124 + +2.1 507,124 + +2.5 507,124 + +0.5 507,125 - -1.1 507,125 - +5.6 507,125 - +0.8 507,126 + +2.7 507,126 + +1.0 507,126 + +0.9 507,126 + +1.1 507,126 + +1.0 507,127 - +1.8 507,127 - +3.6 507,127 - +2.7 507,186 + +1.9 507,187 - +2.1 507,187 - +2.1 507,334 + Pf6N2E2_445 0.25 +1.9 507,334 + Pf6N2E2_445 0.25 +2.2 507,335 - Pf6N2E2_445 0.25 +1.8 507,376 + Pf6N2E2_445 0.30 +2.6 507,421 + Pf6N2E2_445 0.35 +2.6 507,548 + Pf6N2E2_445 0.50 +3.1 507,548 + Pf6N2E2_445 0.50 +2.9 507,571 + Pf6N2E2_445 0.53 +2.2 507,571 + Pf6N2E2_445 0.53 +0.3 507,635 - Pf6N2E2_445 0.60 +2.4 507,673 + Pf6N2E2_445 0.65 +2.5 507,673 + Pf6N2E2_445 0.65 +1.8 507,746 - Pf6N2E2_445 0.74 +2.8 507,862 + Pf6N2E2_445 0.88 +1.5 507,862 + Pf6N2E2_445 0.88 +1.4 507,863 - Pf6N2E2_445 0.88 +2.9 507,863 - Pf6N2E2_445 0.88 +2.8 507,907 + +2.6 507,908 - +2.5 507,908 - +3.3 507,943 + +2.1 507,943 + +0.7 507,943 + +0.5 507,943 + +0.8 507,944 - -0.5 507,944 - -0.7 507,944 - +0.1 507,961 + -0.4 508,137 + +0.5 508,138 - +0.1 508,138 - +0.5 508,138 - +1.8 508,138 - -2.0 508,138 - +1.5 508,162 + -0.1 508,162 + +1.2 508,162 + -1.2 508,163 - -2.8 508,163 - +0.2 508,188 + -1.1 508,188 + +0.9 508,188 + +0.4 508,188 + +2.4 508,188 + -2.3 508,188 + -0.5 508,188 + +0.3 508,188 + +0.7 508,188 + -0.5 508,188 + -0.8 508,188 + -0.7 508,188 + +0.7 508,189 - -0.1 508,189 - -1.7 508,189 - -1.4 508,189 - +0.3 508,189 - +0.1 508,217 - -0.3 508,329 + Pf6N2E2_446 0.12 -1.1 508,330 - Pf6N2E2_446 0.12 -0.3 508,341 + +0.4 508,341 + Pf6N2E2_446 0.13 -0.0 508,371 + Pf6N2E2_446 0.15 +0.9 508,372 - Pf6N2E2_446 0.15 -0.2 508,458 + Pf6N2E2_446 0.21 -0.3 508,750 + Pf6N2E2_446 0.43 -0.2 508,750 + Pf6N2E2_446 0.43 +1.1 508,750 + Pf6N2E2_446 0.43 -0.4 508,750 + Pf6N2E2_446 0.43 +0.1 508,750 + Pf6N2E2_446 0.43 -1.7 508,750 + Pf6N2E2_446 0.43 -0.6 508,750 + Pf6N2E2_446 0.43 +1.7 508,750 + Pf6N2E2_446 0.43 +0.3 508,750 + Pf6N2E2_446 0.43 -0.9 508,750 + Pf6N2E2_446 0.43 +0.2 508,750 + Pf6N2E2_446 0.43 +0.6 508,751 - Pf6N2E2_446 0.43 -0.4 508,751 - Pf6N2E2_446 0.43 -1.4 508,751 - Pf6N2E2_446 0.43 +0.3 508,751 - Pf6N2E2_446 0.43 +0.0 508,751 - Pf6N2E2_446 0.43 -2.1 508,751 - Pf6N2E2_446 0.43 +2.4 508,751 - Pf6N2E2_446 0.43 -0.1 508,938 + Pf6N2E2_446 0.57 -0.2 508,938 + Pf6N2E2_446 0.57 +1.6 508,938 + Pf6N2E2_446 0.57 +0.8 508,939 - Pf6N2E2_446 0.57 -0.6
Or see this region's nucleotide sequence