Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3023 and Pf6N2E2_3024 are separated by 0 nucleotides Pf6N2E2_3024 and Pf6N2E2_3025 overlap by 4 nucleotides Pf6N2E2_3025 and Pf6N2E2_3026 overlap by 4 nucleotides
Pf6N2E2_3023 - Prophage long tail fiber protein, at 3,508,533 to 3,510,611
_3023
Pf6N2E2_3024 - Phage tail fibers, at 3,510,612 to 3,511,247
_3024
Pf6N2E2_3025 - Baseplate assembly protein J, at 3,511,244 to 3,512,239
_3025
Pf6N2E2_3026 - Baseplate assembly protein W, at 3,512,236 to 3,512,568
_3026
Position (kb)
3510
3511
3512 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3509.625 kb on - strand, within Pf6N2E2_3023 at 3509.652 kb on - strand, within Pf6N2E2_3023 at 3509.831 kb on + strand, within Pf6N2E2_3023 at 3509.983 kb on + strand, within Pf6N2E2_3023 at 3509.984 kb on - strand, within Pf6N2E2_3023 at 3509.984 kb on - strand, within Pf6N2E2_3023 at 3509.988 kb on - strand, within Pf6N2E2_3023 at 3509.988 kb on - strand at 3509.988 kb on - strand, within Pf6N2E2_3023 at 3509.988 kb on - strand, within Pf6N2E2_3023 at 3510.084 kb on - strand, within Pf6N2E2_3023 at 3510.298 kb on + strand, within Pf6N2E2_3023 at 3510.355 kb on + strand, within Pf6N2E2_3023 at 3510.355 kb on + strand, within Pf6N2E2_3023 at 3510.356 kb on - strand, within Pf6N2E2_3023 at 3510.490 kb on + strand at 3510.598 kb on + strand at 3510.598 kb on + strand at 3510.598 kb on + strand at 3510.598 kb on + strand at 3510.599 kb on - strand at 3510.613 kb on + strand at 3510.613 kb on + strand at 3510.613 kb on + strand at 3510.614 kb on - strand at 3510.628 kb on + strand at 3510.628 kb on + strand at 3510.628 kb on + strand at 3510.632 kb on + strand at 3510.632 kb on + strand at 3510.632 kb on + strand at 3510.632 kb on + strand at 3510.632 kb on + strand at 3510.632 kb on + strand at 3510.633 kb on - strand at 3510.652 kb on + strand at 3510.859 kb on + strand, within Pf6N2E2_3024 at 3510.859 kb on + strand, within Pf6N2E2_3024 at 3511.091 kb on - strand, within Pf6N2E2_3024 at 3511.247 kb on + strand at 3511.248 kb on - strand at 3511.366 kb on - strand, within Pf6N2E2_3025 at 3511.419 kb on + strand, within Pf6N2E2_3025 at 3511.420 kb on - strand, within Pf6N2E2_3025 at 3511.504 kb on - strand, within Pf6N2E2_3025 at 3511.716 kb on + strand, within Pf6N2E2_3025 at 3511.716 kb on + strand, within Pf6N2E2_3025 at 3511.716 kb on + strand, within Pf6N2E2_3025 at 3511.717 kb on - strand, within Pf6N2E2_3025 at 3511.717 kb on - strand, within Pf6N2E2_3025 at 3511.730 kb on + strand, within Pf6N2E2_3025 at 3511.730 kb on + strand, within Pf6N2E2_3025 at 3511.730 kb on + strand, within Pf6N2E2_3025 at 3511.731 kb on - strand, within Pf6N2E2_3025 at 3511.780 kb on + strand, within Pf6N2E2_3025 at 3511.840 kb on - strand, within Pf6N2E2_3025 at 3511.840 kb on - strand, within Pf6N2E2_3025 at 3511.873 kb on - strand, within Pf6N2E2_3025 at 3512.010 kb on + strand, within Pf6N2E2_3025 at 3512.010 kb on + strand, within Pf6N2E2_3025 at 3512.011 kb on - strand, within Pf6N2E2_3025 at 3512.011 kb on - strand, within Pf6N2E2_3025 at 3512.011 kb on - strand, within Pf6N2E2_3025 at 3512.133 kb on + strand, within Pf6N2E2_3025 at 3512.133 kb on + strand, within Pf6N2E2_3025 at 3512.133 kb on + strand, within Pf6N2E2_3025 at 3512.134 kb on - strand, within Pf6N2E2_3025
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 3,509,625 - Pf6N2E2_3023 0.53 +0.4 3,509,652 - Pf6N2E2_3023 0.54 +0.5 3,509,831 + Pf6N2E2_3023 0.62 -0.2 3,509,983 + Pf6N2E2_3023 0.70 +0.7 3,509,984 - Pf6N2E2_3023 0.70 +0.9 3,509,984 - Pf6N2E2_3023 0.70 -1.5 3,509,988 - Pf6N2E2_3023 0.70 -0.1 3,509,988 - +2.5 3,509,988 - Pf6N2E2_3023 0.70 -0.2 3,509,988 - Pf6N2E2_3023 0.70 -0.2 3,510,084 - Pf6N2E2_3023 0.75 -1.1 3,510,298 + Pf6N2E2_3023 0.85 +1.3 3,510,355 + Pf6N2E2_3023 0.88 +0.8 3,510,355 + Pf6N2E2_3023 0.88 +0.5 3,510,356 - Pf6N2E2_3023 0.88 -0.1 3,510,490 + +0.9 3,510,598 + -0.1 3,510,598 + -0.2 3,510,598 + +0.7 3,510,598 + +0.8 3,510,599 - -0.3 3,510,613 + -0.8 3,510,613 + -0.5 3,510,613 + +0.4 3,510,614 - -0.8 3,510,628 + -0.3 3,510,628 + +0.6 3,510,628 + +0.2 3,510,632 + +0.2 3,510,632 + -2.3 3,510,632 + -0.5 3,510,632 + +0.8 3,510,632 + -2.5 3,510,632 + +0.6 3,510,633 - +0.4 3,510,652 + +1.3 3,510,859 + Pf6N2E2_3024 0.39 +0.4 3,510,859 + Pf6N2E2_3024 0.39 +1.1 3,511,091 - Pf6N2E2_3024 0.75 +0.1 3,511,247 + +1.5 3,511,248 - -0.8 3,511,366 - Pf6N2E2_3025 0.12 -1.8 3,511,419 + Pf6N2E2_3025 0.18 +1.0 3,511,420 - Pf6N2E2_3025 0.18 +0.1 3,511,504 - Pf6N2E2_3025 0.26 -1.9 3,511,716 + Pf6N2E2_3025 0.47 -0.6 3,511,716 + Pf6N2E2_3025 0.47 -0.2 3,511,716 + Pf6N2E2_3025 0.47 +0.4 3,511,717 - Pf6N2E2_3025 0.47 +0.0 3,511,717 - Pf6N2E2_3025 0.47 -2.1 3,511,730 + Pf6N2E2_3025 0.49 -0.1 3,511,730 + Pf6N2E2_3025 0.49 +0.9 3,511,730 + Pf6N2E2_3025 0.49 +1.3 3,511,731 - Pf6N2E2_3025 0.49 +2.3 3,511,780 + Pf6N2E2_3025 0.54 -0.0 3,511,840 - Pf6N2E2_3025 0.60 +0.1 3,511,840 - Pf6N2E2_3025 0.60 +0.7 3,511,873 - Pf6N2E2_3025 0.63 +0.6 3,512,010 + Pf6N2E2_3025 0.77 -1.3 3,512,010 + Pf6N2E2_3025 0.77 +0.1 3,512,011 - Pf6N2E2_3025 0.77 +0.4 3,512,011 - Pf6N2E2_3025 0.77 -3.0 3,512,011 - Pf6N2E2_3025 0.77 +0.5 3,512,133 + Pf6N2E2_3025 0.89 -0.3 3,512,133 + Pf6N2E2_3025 0.89 +0.7 3,512,133 + Pf6N2E2_3025 0.89 +0.7 3,512,134 - Pf6N2E2_3025 0.89 +0.1
Or see this region's nucleotide sequence