Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2773 and Pf6N2E2_2774 are separated by 64 nucleotides Pf6N2E2_2774 and Pf6N2E2_2775 are separated by 800 nucleotides Pf6N2E2_2775 and Pf6N2E2_2776 are separated by 15 nucleotides Pf6N2E2_2776 and Pf6N2E2_2777 overlap by 4 nucleotides
Pf6N2E2_2773 - Enoyl-CoA hydratase (EC 4.2.1.17), at 3,261,340 to 3,262,452
_2773
Pf6N2E2_2774 - hypothetical protein, at 3,262,517 to 3,262,639
_2774
Pf6N2E2_2775 - Urea carboxylase-related ABC transporter, periplasmic substrate-binding protein, at 3,263,440 to 3,264,507
_2775
Pf6N2E2_2776 - Urea carboxylase-related ABC transporter, permease protein, at 3,264,523 to 3,265,338
_2776
Pf6N2E2_2777 - Urea carboxylase-related ABC transporter, ATPase protein, at 3,265,335 to 3,266,123
_2777
Position (kb)
3263
3264
3265 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3262.478 kb on - strand at 3262.492 kb on - strand at 3262.492 kb on - strand at 3262.492 kb on - strand at 3262.696 kb on + strand at 3262.697 kb on - strand at 3262.697 kb on - strand at 3262.700 kb on - strand at 3262.717 kb on + strand at 3262.717 kb on + strand at 3262.717 kb on + strand at 3262.717 kb on + strand at 3262.721 kb on - strand at 3262.721 kb on - strand at 3262.735 kb on + strand at 3262.736 kb on - strand at 3262.756 kb on + strand at 3262.756 kb on + strand at 3262.756 kb on + strand at 3262.757 kb on - strand at 3262.759 kb on + strand at 3262.759 kb on + strand at 3262.777 kb on + strand at 3262.778 kb on - strand at 3262.778 kb on - strand at 3262.778 kb on - strand at 3262.795 kb on + strand at 3262.795 kb on + strand at 3262.795 kb on + strand at 3262.796 kb on - strand at 3262.810 kb on + strand at 3262.810 kb on + strand at 3262.811 kb on - strand at 3262.811 kb on - strand at 3262.811 kb on - strand at 3262.835 kb on - strand at 3262.835 kb on - strand at 3262.835 kb on - strand at 3262.849 kb on + strand at 3262.849 kb on + strand at 3262.849 kb on + strand at 3262.850 kb on - strand at 3262.850 kb on - strand at 3262.852 kb on + strand at 3262.852 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.888 kb on + strand at 3262.889 kb on - strand at 3262.891 kb on + strand at 3262.891 kb on + strand at 3262.891 kb on + strand at 3262.891 kb on + strand at 3262.891 kb on + strand at 3262.892 kb on - strand at 3262.892 kb on - strand at 3262.892 kb on - strand at 3262.909 kb on + strand at 3262.910 kb on - strand at 3262.912 kb on + strand at 3262.913 kb on - strand at 3262.969 kb on + strand at 3262.970 kb on - strand at 3262.970 kb on - strand at 3262.970 kb on - strand at 3263.050 kb on - strand at 3263.050 kb on - strand at 3263.094 kb on - strand at 3263.353 kb on - strand at 3263.548 kb on + strand, within Pf6N2E2_2775 at 3263.548 kb on + strand, within Pf6N2E2_2775 at 3263.548 kb on + strand, within Pf6N2E2_2775 at 3263.549 kb on - strand, within Pf6N2E2_2775 at 3263.549 kb on - strand, within Pf6N2E2_2775 at 3263.549 kb on - strand, within Pf6N2E2_2775 at 3263.572 kb on + strand, within Pf6N2E2_2775 at 3263.614 kb on + strand, within Pf6N2E2_2775 at 3263.650 kb on + strand, within Pf6N2E2_2775 at 3263.671 kb on + strand, within Pf6N2E2_2775 at 3263.672 kb on - strand, within Pf6N2E2_2775 at 3263.672 kb on - strand, within Pf6N2E2_2775 at 3263.672 kb on - strand, within Pf6N2E2_2775 at 3263.875 kb on + strand, within Pf6N2E2_2775 at 3263.875 kb on + strand, within Pf6N2E2_2775 at 3263.876 kb on - strand, within Pf6N2E2_2775 at 3263.876 kb on - strand, within Pf6N2E2_2775 at 3263.937 kb on + strand, within Pf6N2E2_2775 at 3263.937 kb on + strand, within Pf6N2E2_2775 at 3264.475 kb on + strand at 3264.475 kb on + strand at 3264.475 kb on + strand at 3264.475 kb on + strand at 3264.479 kb on + strand at 3264.479 kb on + strand at 3264.480 kb on - strand at 3264.480 kb on - strand at 3264.480 kb on - strand at 3264.480 kb on - strand at 3264.563 kb on + strand at 3264.563 kb on + strand at 3264.563 kb on + strand at 3264.563 kb on + strand at 3264.727 kb on + strand, within Pf6N2E2_2776 at 3264.727 kb on + strand, within Pf6N2E2_2776 at 3264.728 kb on - strand, within Pf6N2E2_2776 at 3264.728 kb on - strand, within Pf6N2E2_2776 at 3265.127 kb on + strand, within Pf6N2E2_2776 at 3265.127 kb on + strand, within Pf6N2E2_2776 at 3265.127 kb on + strand, within Pf6N2E2_2776 at 3265.127 kb on + strand, within Pf6N2E2_2776 at 3265.127 kb on + strand, within Pf6N2E2_2776 at 3265.127 kb on + strand, within Pf6N2E2_2776 at 3265.128 kb on - strand, within Pf6N2E2_2776 at 3265.214 kb on - strand, within Pf6N2E2_2776 at 3265.241 kb on - strand, within Pf6N2E2_2776 at 3265.318 kb on + strand at 3265.319 kb on - strand at 3265.319 kb on - strand at 3265.371 kb on + strand at 3265.371 kb on + strand at 3265.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 3,262,478 - -0.3 3,262,492 - +0.6 3,262,492 - +0.4 3,262,492 - +0.5 3,262,696 + -1.4 3,262,697 - +0.6 3,262,697 - +0.2 3,262,700 - -0.2 3,262,717 + +0.1 3,262,717 + -0.1 3,262,717 + -0.2 3,262,717 + -1.2 3,262,721 - -0.9 3,262,721 - +0.1 3,262,735 + +0.7 3,262,736 - -0.0 3,262,756 + +1.8 3,262,756 + -0.6 3,262,756 + -0.3 3,262,757 - -0.9 3,262,759 + +0.6 3,262,759 + +1.0 3,262,777 + -0.8 3,262,778 - +0.8 3,262,778 - +0.7 3,262,778 - -0.2 3,262,795 + +0.5 3,262,795 + +1.1 3,262,795 + +0.3 3,262,796 - +1.0 3,262,810 + -0.3 3,262,810 + +1.1 3,262,811 - +1.0 3,262,811 - -1.6 3,262,811 - +0.5 3,262,835 - -0.2 3,262,835 - +0.2 3,262,835 - -1.0 3,262,849 + -0.5 3,262,849 + +0.7 3,262,849 + +0.2 3,262,850 - -1.0 3,262,850 - +0.3 3,262,852 + -1.1 3,262,852 + +0.7 3,262,871 + +0.4 3,262,871 + +0.1 3,262,871 + -0.2 3,262,871 + -0.0 3,262,871 + +0.2 3,262,871 + +1.2 3,262,872 - -2.5 3,262,872 - +0.5 3,262,872 - -0.6 3,262,872 - -0.6 3,262,872 - -0.0 3,262,872 - +1.2 3,262,872 - +0.7 3,262,873 + +0.6 3,262,873 + -2.2 3,262,873 + +0.9 3,262,873 + -1.6 3,262,873 + -1.7 3,262,873 + -1.0 3,262,874 - -2.8 3,262,874 - +1.6 3,262,874 - +1.2 3,262,874 - +0.8 3,262,874 - +0.3 3,262,874 - -0.1 3,262,874 - +0.0 3,262,888 + +1.4 3,262,889 - +0.3 3,262,891 + +1.6 3,262,891 + +0.1 3,262,891 + +0.2 3,262,891 + -1.8 3,262,891 + +0.1 3,262,892 - -0.0 3,262,892 - -0.0 3,262,892 - +0.5 3,262,909 + -0.6 3,262,910 - +0.6 3,262,912 + -0.2 3,262,913 - -2.0 3,262,969 + +0.4 3,262,970 - +1.0 3,262,970 - -0.9 3,262,970 - -0.5 3,263,050 - +0.2 3,263,050 - -0.8 3,263,094 - +0.5 3,263,353 - -0.3 3,263,548 + Pf6N2E2_2775 0.10 +1.0 3,263,548 + Pf6N2E2_2775 0.10 +3.0 3,263,548 + Pf6N2E2_2775 0.10 -1.1 3,263,549 - Pf6N2E2_2775 0.10 -0.5 3,263,549 - Pf6N2E2_2775 0.10 -0.3 3,263,549 - Pf6N2E2_2775 0.10 -1.0 3,263,572 + Pf6N2E2_2775 0.12 +0.3 3,263,614 + Pf6N2E2_2775 0.16 -0.2 3,263,650 + Pf6N2E2_2775 0.20 -1.7 3,263,671 + Pf6N2E2_2775 0.22 +0.6 3,263,672 - Pf6N2E2_2775 0.22 -0.7 3,263,672 - Pf6N2E2_2775 0.22 +1.0 3,263,672 - Pf6N2E2_2775 0.22 -0.3 3,263,875 + Pf6N2E2_2775 0.41 -0.0 3,263,875 + Pf6N2E2_2775 0.41 +1.3 3,263,876 - Pf6N2E2_2775 0.41 +1.1 3,263,876 - Pf6N2E2_2775 0.41 -0.5 3,263,937 + Pf6N2E2_2775 0.47 -0.8 3,263,937 + Pf6N2E2_2775 0.47 -1.6 3,264,475 + +0.1 3,264,475 + +1.0 3,264,475 + -1.4 3,264,475 + -0.4 3,264,479 + -1.5 3,264,479 + +1.0 3,264,480 - -2.7 3,264,480 - -0.5 3,264,480 - +1.4 3,264,480 - -0.7 3,264,563 + -0.4 3,264,563 + -0.2 3,264,563 + +1.0 3,264,563 + -0.9 3,264,727 + Pf6N2E2_2776 0.25 +1.3 3,264,727 + Pf6N2E2_2776 0.25 +0.5 3,264,728 - Pf6N2E2_2776 0.25 +1.7 3,264,728 - Pf6N2E2_2776 0.25 -0.2 3,265,127 + Pf6N2E2_2776 0.74 -0.2 3,265,127 + Pf6N2E2_2776 0.74 +0.5 3,265,127 + Pf6N2E2_2776 0.74 -0.2 3,265,127 + Pf6N2E2_2776 0.74 -1.3 3,265,127 + Pf6N2E2_2776 0.74 +0.7 3,265,127 + Pf6N2E2_2776 0.74 +0.9 3,265,128 - Pf6N2E2_2776 0.74 +1.8 3,265,214 - Pf6N2E2_2776 0.85 -0.3 3,265,241 - Pf6N2E2_2776 0.88 +0.5 3,265,318 + +1.0 3,265,319 - -0.4 3,265,319 - -1.0 3,265,371 + -1.0 3,265,371 + -1.5 3,265,371 + -1.1
Or see this region's nucleotide sequence