Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2361 and Pf6N2E2_2362 are separated by 475 nucleotides Pf6N2E2_2362 and Pf6N2E2_2363 overlap by 26 nucleotides Pf6N2E2_2363 and Pf6N2E2_2364 are separated by 4 nucleotides
Pf6N2E2_2361 - Type III secretion chaperone protein for YopD (SycD), at 2,796,383 to 2,796,865
_2361
Pf6N2E2_2362 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 2,797,341 to 2,798,375
_2362
Pf6N2E2_2363 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 2,798,350 to 2,799,153
_2363
Pf6N2E2_2364 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 2,799,158 to 2,799,412
_2364
Position (kb)
2797
2798
2799 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.429 kb on + strand at 2796.430 kb on - strand at 2796.430 kb on - strand at 2796.430 kb on - strand at 2796.430 kb on - strand at 2796.480 kb on + strand, within Pf6N2E2_2361 at 2796.481 kb on - strand, within Pf6N2E2_2361 at 2796.481 kb on - strand, within Pf6N2E2_2361 at 2796.481 kb on - strand, within Pf6N2E2_2361 at 2796.540 kb on + strand, within Pf6N2E2_2361 at 2796.541 kb on - strand, within Pf6N2E2_2361 at 2796.589 kb on - strand, within Pf6N2E2_2361 at 2796.589 kb on - strand, within Pf6N2E2_2361 at 2796.651 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.664 kb on - strand, within Pf6N2E2_2361 at 2796.673 kb on - strand, within Pf6N2E2_2361 at 2796.726 kb on + strand, within Pf6N2E2_2361 at 2796.726 kb on + strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.747 kb on + strand, within Pf6N2E2_2361 at 2797.249 kb on + strand at 2797.417 kb on + strand at 2797.417 kb on + strand at 2797.492 kb on + strand, within Pf6N2E2_2362 at 2797.492 kb on + strand, within Pf6N2E2_2362 at 2797.492 kb on + strand, within Pf6N2E2_2362 at 2797.570 kb on + strand, within Pf6N2E2_2362 at 2797.570 kb on + strand, within Pf6N2E2_2362 at 2797.766 kb on - strand, within Pf6N2E2_2362 at 2797.948 kb on + strand, within Pf6N2E2_2362 at 2797.949 kb on - strand, within Pf6N2E2_2362 at 2798.207 kb on - strand, within Pf6N2E2_2362 at 2798.248 kb on + strand, within Pf6N2E2_2362 at 2798.249 kb on - strand, within Pf6N2E2_2362 at 2798.465 kb on + strand, within Pf6N2E2_2363 at 2798.659 kb on + strand, within Pf6N2E2_2363 at 2798.693 kb on + strand, within Pf6N2E2_2363 at 2798.745 kb on - strand, within Pf6N2E2_2363 at 2798.908 kb on + strand, within Pf6N2E2_2363 at 2798.985 kb on - strand, within Pf6N2E2_2363 at 2799.125 kb on + strand at 2799.126 kb on - strand at 2799.159 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 2,796,369 + +1.2 2,796,369 + -0.2 2,796,369 + -0.1 2,796,369 + +0.4 2,796,369 + +0.7 2,796,369 + -0.7 2,796,370 - -2.8 2,796,370 - -0.0 2,796,370 - +0.2 2,796,370 - +0.1 2,796,370 - -0.7 2,796,370 - -0.4 2,796,429 + -2.7 2,796,430 - +0.7 2,796,430 - +1.3 2,796,430 - +1.3 2,796,430 - +1.0 2,796,480 + Pf6N2E2_2361 0.20 +0.5 2,796,481 - Pf6N2E2_2361 0.20 -1.8 2,796,481 - Pf6N2E2_2361 0.20 +0.4 2,796,481 - Pf6N2E2_2361 0.20 -1.4 2,796,540 + Pf6N2E2_2361 0.33 -0.4 2,796,541 - Pf6N2E2_2361 0.33 +0.1 2,796,589 - Pf6N2E2_2361 0.43 +0.9 2,796,589 - Pf6N2E2_2361 0.43 +1.4 2,796,651 + Pf6N2E2_2361 0.55 -1.2 2,796,663 + Pf6N2E2_2361 0.58 +1.2 2,796,663 + Pf6N2E2_2361 0.58 +0.4 2,796,663 + Pf6N2E2_2361 0.58 +1.1 2,796,663 + Pf6N2E2_2361 0.58 -0.8 2,796,664 - Pf6N2E2_2361 0.58 +1.0 2,796,673 - Pf6N2E2_2361 0.60 -0.1 2,796,726 + Pf6N2E2_2361 0.71 -0.2 2,796,726 + Pf6N2E2_2361 0.71 -0.7 2,796,727 - Pf6N2E2_2361 0.71 +0.1 2,796,727 - Pf6N2E2_2361 0.71 -1.1 2,796,727 - Pf6N2E2_2361 0.71 +0.4 2,796,727 - Pf6N2E2_2361 0.71 -0.2 2,796,747 + Pf6N2E2_2361 0.75 -1.3 2,797,249 + +0.4 2,797,417 + -0.1 2,797,417 + -0.3 2,797,492 + Pf6N2E2_2362 0.15 +0.5 2,797,492 + Pf6N2E2_2362 0.15 -0.3 2,797,492 + Pf6N2E2_2362 0.15 +0.8 2,797,570 + Pf6N2E2_2362 0.22 -0.4 2,797,570 + Pf6N2E2_2362 0.22 +1.1 2,797,766 - Pf6N2E2_2362 0.41 -0.1 2,797,948 + Pf6N2E2_2362 0.59 +1.2 2,797,949 - Pf6N2E2_2362 0.59 +0.5 2,798,207 - Pf6N2E2_2362 0.84 +1.3 2,798,248 + Pf6N2E2_2362 0.88 +1.1 2,798,249 - Pf6N2E2_2362 0.88 +0.6 2,798,465 + Pf6N2E2_2363 0.14 +0.3 2,798,659 + Pf6N2E2_2363 0.38 +0.1 2,798,693 + Pf6N2E2_2363 0.43 +1.9 2,798,745 - Pf6N2E2_2363 0.49 +1.0 2,798,908 + Pf6N2E2_2363 0.69 +0.3 2,798,985 - Pf6N2E2_2363 0.79 -0.1 2,799,125 + +1.1 2,799,126 - -0.2 2,799,159 + -0.1
Or see this region's nucleotide sequence