Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_222 and Pf6N2E2_223 overlap by 4 nucleotides Pf6N2E2_223 and Pf6N2E2_224 are separated by 242 nucleotides
Pf6N2E2_222 - RND efflux system, inner membrane transporter CmeB, at 280,435 to 283,581
_222
Pf6N2E2_223 - RND efflux system, outer membrane lipoprotein CmeC, at 283,578 to 284,975
_223
Pf6N2E2_224 - transcription factor nitR, at 285,218 to 286,216
_224
Position (kb) 283
284
285 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.700 kb on + strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.701 kb on - strand, within Pf6N2E2_222 at 282.742 kb on + strand, within Pf6N2E2_222 at 282.742 kb on + strand, within Pf6N2E2_222 at 282.743 kb on - strand, within Pf6N2E2_222 at 282.743 kb on - strand, within Pf6N2E2_222 at 282.859 kb on + strand, within Pf6N2E2_222 at 282.860 kb on - strand, within Pf6N2E2_222 at 282.884 kb on - strand, within Pf6N2E2_222 at 283.018 kb on + strand, within Pf6N2E2_222 at 283.019 kb on - strand, within Pf6N2E2_222 at 283.057 kb on + strand, within Pf6N2E2_222 at 283.080 kb on - strand, within Pf6N2E2_222 at 283.204 kb on + strand, within Pf6N2E2_222 at 283.382 kb on + strand at 283.650 kb on + strand at 283.650 kb on + strand at 283.651 kb on - strand at 283.651 kb on - strand at 283.959 kb on + strand, within Pf6N2E2_223 at 283.959 kb on + strand, within Pf6N2E2_223 at 283.959 kb on + strand, within Pf6N2E2_223 at 283.959 kb on + strand, within Pf6N2E2_223 at 283.986 kb on + strand, within Pf6N2E2_223 at 283.987 kb on - strand, within Pf6N2E2_223 at 283.987 kb on - strand, within Pf6N2E2_223 at 283.987 kb on - strand, within Pf6N2E2_223 at 283.987 kb on - strand, within Pf6N2E2_223 at 284.043 kb on + strand, within Pf6N2E2_223 at 284.044 kb on - strand, within Pf6N2E2_223 at 284.181 kb on + strand, within Pf6N2E2_223 at 284.181 kb on + strand, within Pf6N2E2_223 at 284.249 kb on + strand, within Pf6N2E2_223 at 284.367 kb on + strand, within Pf6N2E2_223 at 284.691 kb on + strand, within Pf6N2E2_223 at 284.692 kb on - strand, within Pf6N2E2_223 at 284.692 kb on - strand, within Pf6N2E2_223 at 284.850 kb on + strand at 284.883 kb on + strand at 284.884 kb on - strand at 284.946 kb on + strand at 284.946 kb on + strand at 284.946 kb on + strand at 284.947 kb on - strand at 284.947 kb on - strand at 284.974 kb on - strand at 284.974 kb on - strand at 284.974 kb on - strand at 284.994 kb on + strand at 284.995 kb on - strand at 285.189 kb on - strand at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.697 kb on - strand, within Pf6N2E2_224 at 285.748 kb on + strand, within Pf6N2E2_224 at 285.952 kb on + strand, within Pf6N2E2_224
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 282,700 + Pf6N2E2_222 0.72 -0.2 282,700 + Pf6N2E2_222 0.72 +0.6 282,700 + Pf6N2E2_222 0.72 +0.3 282,700 + Pf6N2E2_222 0.72 +1.4 282,700 + Pf6N2E2_222 0.72 +0.3 282,700 + Pf6N2E2_222 0.72 -0.4 282,700 + Pf6N2E2_222 0.72 -1.6 282,700 + Pf6N2E2_222 0.72 +0.0 282,700 + Pf6N2E2_222 0.72 +0.2 282,700 + Pf6N2E2_222 0.72 -0.7 282,701 - Pf6N2E2_222 0.72 -0.0 282,701 - Pf6N2E2_222 0.72 +0.6 282,701 - Pf6N2E2_222 0.72 -0.1 282,701 - Pf6N2E2_222 0.72 +0.2 282,701 - Pf6N2E2_222 0.72 -0.5 282,742 + Pf6N2E2_222 0.73 -0.5 282,742 + Pf6N2E2_222 0.73 -1.7 282,743 - Pf6N2E2_222 0.73 -0.2 282,743 - Pf6N2E2_222 0.73 -1.3 282,859 + Pf6N2E2_222 0.77 +0.5 282,860 - Pf6N2E2_222 0.77 -0.1 282,884 - Pf6N2E2_222 0.78 -0.3 283,018 + Pf6N2E2_222 0.82 -0.3 283,019 - Pf6N2E2_222 0.82 -0.4 283,057 + Pf6N2E2_222 0.83 +0.4 283,080 - Pf6N2E2_222 0.84 +0.7 283,204 + Pf6N2E2_222 0.88 +0.9 283,382 + +0.8 283,650 + +0.1 283,650 + -2.0 283,651 - -0.6 283,651 - -0.3 283,959 + Pf6N2E2_223 0.27 +0.3 283,959 + Pf6N2E2_223 0.27 +0.6 283,959 + Pf6N2E2_223 0.27 -1.2 283,959 + Pf6N2E2_223 0.27 +0.7 283,986 + Pf6N2E2_223 0.29 +0.7 283,987 - Pf6N2E2_223 0.29 -0.1 283,987 - Pf6N2E2_223 0.29 +0.1 283,987 - Pf6N2E2_223 0.29 -0.1 283,987 - Pf6N2E2_223 0.29 -1.3 284,043 + Pf6N2E2_223 0.33 +0.4 284,044 - Pf6N2E2_223 0.33 -1.8 284,181 + Pf6N2E2_223 0.43 -0.3 284,181 + Pf6N2E2_223 0.43 -0.0 284,249 + Pf6N2E2_223 0.48 -0.6 284,367 + Pf6N2E2_223 0.56 -0.3 284,691 + Pf6N2E2_223 0.80 +0.2 284,692 - Pf6N2E2_223 0.80 -1.2 284,692 - Pf6N2E2_223 0.80 -0.2 284,850 + +0.3 284,883 + +0.1 284,884 - +0.4 284,946 + -0.7 284,946 + +0.6 284,946 + +0.6 284,947 - -0.6 284,947 - -0.7 284,974 - +0.1 284,974 - -0.1 284,974 - +1.3 284,994 + +1.0 284,995 - -0.4 285,189 - +1.1 285,423 + Pf6N2E2_224 0.21 +0.7 285,423 + Pf6N2E2_224 0.21 +2.8 285,423 + Pf6N2E2_224 0.21 +0.7 285,424 - Pf6N2E2_224 0.21 +1.5 285,424 - Pf6N2E2_224 0.21 -0.2 285,697 - Pf6N2E2_224 0.48 +1.7 285,748 + Pf6N2E2_224 0.53 -1.3 285,952 + Pf6N2E2_224 0.73 -1.1
Or see this region's nucleotide sequence