Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1525 and Pf6N2E2_1526 are separated by 100 nucleotides Pf6N2E2_1526 and Pf6N2E2_1527 are separated by 257 nucleotides
Pf6N2E2_1525 - Universal stress protein family 5, at 1,782,830 to 1,783,741
_1525
Pf6N2E2_1526 - MgtC family, at 1,783,842 to 1,785,092
_1526
Pf6N2E2_1527 - Universal stress protein family 5, at 1,785,350 to 1,786,273
_1527
Position (kb)
1783
1784
1785
1786 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1783.087 kb on - strand, within Pf6N2E2_1525 at 1783.135 kb on - strand at 1783.434 kb on + strand, within Pf6N2E2_1525 at 1783.435 kb on - strand, within Pf6N2E2_1525 at 1783.435 kb on - strand, within Pf6N2E2_1525 at 1783.435 kb on - strand, within Pf6N2E2_1525 at 1783.552 kb on - strand, within Pf6N2E2_1525 at 1783.633 kb on + strand, within Pf6N2E2_1525 at 1783.633 kb on + strand, within Pf6N2E2_1525 at 1783.634 kb on - strand, within Pf6N2E2_1525 at 1783.722 kb on - strand at 1783.722 kb on - strand at 1783.772 kb on + strand at 1783.773 kb on - strand at 1783.911 kb on + strand at 1783.915 kb on + strand at 1783.915 kb on + strand at 1784.266 kb on + strand, within Pf6N2E2_1526 at 1784.267 kb on - strand, within Pf6N2E2_1526 at 1784.267 kb on - strand, within Pf6N2E2_1526 at 1784.275 kb on - strand, within Pf6N2E2_1526 at 1784.275 kb on - strand, within Pf6N2E2_1526 at 1784.503 kb on + strand, within Pf6N2E2_1526 at 1784.602 kb on + strand, within Pf6N2E2_1526 at 1784.603 kb on - strand, within Pf6N2E2_1526 at 1784.603 kb on - strand, within Pf6N2E2_1526 at 1784.809 kb on - strand, within Pf6N2E2_1526 at 1784.972 kb on + strand at 1784.972 kb on + strand at 1784.973 kb on - strand at 1785.258 kb on - strand at 1785.258 kb on - strand at 1785.355 kb on + strand at 1785.355 kb on + strand at 1785.457 kb on + strand, within Pf6N2E2_1527 at 1785.458 kb on - strand, within Pf6N2E2_1527 at 1785.458 kb on - strand, within Pf6N2E2_1527 at 1785.458 kb on - strand, within Pf6N2E2_1527 at 1785.458 kb on - strand, within Pf6N2E2_1527 at 1785.473 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.968 kb on + strand, within Pf6N2E2_1527 at 1786.022 kb on + strand, within Pf6N2E2_1527 at 1786.023 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 1,783,087 - Pf6N2E2_1525 0.28 +0.9 1,783,135 - -1.4 1,783,434 + Pf6N2E2_1525 0.66 +0.8 1,783,435 - Pf6N2E2_1525 0.66 +1.2 1,783,435 - Pf6N2E2_1525 0.66 -0.1 1,783,435 - Pf6N2E2_1525 0.66 +0.4 1,783,552 - Pf6N2E2_1525 0.79 +0.4 1,783,633 + Pf6N2E2_1525 0.88 -0.8 1,783,633 + Pf6N2E2_1525 0.88 +0.6 1,783,634 - Pf6N2E2_1525 0.88 -0.7 1,783,722 - -1.1 1,783,722 - +0.3 1,783,772 + -0.2 1,783,773 - -0.0 1,783,911 + +0.1 1,783,915 + -1.8 1,783,915 + -1.0 1,784,266 + Pf6N2E2_1526 0.34 +1.8 1,784,267 - Pf6N2E2_1526 0.34 +1.6 1,784,267 - Pf6N2E2_1526 0.34 -2.4 1,784,275 - Pf6N2E2_1526 0.35 +0.1 1,784,275 - Pf6N2E2_1526 0.35 +1.2 1,784,503 + Pf6N2E2_1526 0.53 -0.0 1,784,602 + Pf6N2E2_1526 0.61 +1.0 1,784,603 - Pf6N2E2_1526 0.61 +0.9 1,784,603 - Pf6N2E2_1526 0.61 +0.3 1,784,809 - Pf6N2E2_1526 0.77 +0.5 1,784,972 + -1.0 1,784,972 + -0.1 1,784,973 - +0.2 1,785,258 - +0.0 1,785,258 - +0.2 1,785,355 + -1.2 1,785,355 + -0.4 1,785,457 + Pf6N2E2_1527 0.12 +0.2 1,785,458 - Pf6N2E2_1527 0.12 +0.1 1,785,458 - Pf6N2E2_1527 0.12 +0.0 1,785,458 - Pf6N2E2_1527 0.12 +0.4 1,785,458 - Pf6N2E2_1527 0.12 +2.2 1,785,473 + Pf6N2E2_1527 0.13 +0.5 1,785,932 + Pf6N2E2_1527 0.63 +1.2 1,785,932 + Pf6N2E2_1527 0.63 +1.2 1,785,932 + Pf6N2E2_1527 0.63 -2.4 1,785,932 + Pf6N2E2_1527 0.63 -0.0 1,785,932 + Pf6N2E2_1527 0.63 +0.4 1,785,932 + Pf6N2E2_1527 0.63 +0.1 1,785,932 + Pf6N2E2_1527 0.63 +0.4 1,785,933 - Pf6N2E2_1527 0.63 +1.1 1,785,933 - Pf6N2E2_1527 0.63 -0.9 1,785,933 - Pf6N2E2_1527 0.63 +0.4 1,785,933 - Pf6N2E2_1527 0.63 +0.8 1,785,933 - Pf6N2E2_1527 0.63 +0.1 1,785,933 - Pf6N2E2_1527 0.63 +0.2 1,785,933 - Pf6N2E2_1527 0.63 +0.6 1,785,968 + Pf6N2E2_1527 0.67 -0.5 1,786,022 + Pf6N2E2_1527 0.73 -1.1 1,786,023 - -0.5
Or see this region's nucleotide sequence