Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1283 and Pf6N2E2_1284 are separated by 35 nucleotides Pf6N2E2_1284 and Pf6N2E2_1285 are separated by 10 nucleotides Pf6N2E2_1285 and Pf6N2E2_1286 are separated by 17 nucleotides
Pf6N2E2_1283 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,513,207 to 1,513,878
_1283
Pf6N2E2_1284 - Biopolymer transport protein ExbD/TolR, at 1,513,914 to 1,514,339
_1284
Pf6N2E2_1285 - MotA/TolQ/ExbB proton channel family protein, at 1,514,350 to 1,516,149
_1285
Pf6N2E2_1286 - Hemolysin activation/secretion protein associated with VreARI signalling system, at 1,516,167 to 1,517,771
_1286
Position (kb)
1514
1515
1516
1517 Strain fitness (log2 ratio)
-2
-1
0
1 at 1513.472 kb on + strand, within Pf6N2E2_1283 at 1513.472 kb on + strand, within Pf6N2E2_1283 at 1513.473 kb on - strand, within Pf6N2E2_1283 at 1513.473 kb on - strand, within Pf6N2E2_1283 at 1514.002 kb on + strand, within Pf6N2E2_1284 at 1514.328 kb on + strand at 1514.508 kb on - strand at 1514.508 kb on - strand at 1514.634 kb on + strand, within Pf6N2E2_1285 at 1514.635 kb on - strand, within Pf6N2E2_1285 at 1514.635 kb on - strand, within Pf6N2E2_1285 at 1514.635 kb on - strand, within Pf6N2E2_1285 at 1514.635 kb on - strand, within Pf6N2E2_1285 at 1514.843 kb on + strand, within Pf6N2E2_1285 at 1514.843 kb on + strand, within Pf6N2E2_1285 at 1514.844 kb on - strand, within Pf6N2E2_1285 at 1514.844 kb on - strand, within Pf6N2E2_1285 at 1514.845 kb on + strand, within Pf6N2E2_1285 at 1514.846 kb on - strand, within Pf6N2E2_1285 at 1515.122 kb on + strand, within Pf6N2E2_1285 at 1515.329 kb on + strand, within Pf6N2E2_1285 at 1515.531 kb on + strand, within Pf6N2E2_1285 at 1515.719 kb on + strand, within Pf6N2E2_1285 at 1515.720 kb on - strand, within Pf6N2E2_1285 at 1515.720 kb on - strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.791 kb on + strand, within Pf6N2E2_1285 at 1515.792 kb on - strand, within Pf6N2E2_1285 at 1516.123 kb on - strand at 1516.138 kb on + strand at 1516.138 kb on + strand at 1516.379 kb on - strand, within Pf6N2E2_1286 at 1516.399 kb on + strand, within Pf6N2E2_1286 at 1516.399 kb on + strand, within Pf6N2E2_1286 at 1516.606 kb on + strand, within Pf6N2E2_1286 at 1516.768 kb on + strand, within Pf6N2E2_1286 at 1516.789 kb on + strand, within Pf6N2E2_1286 at 1516.903 kb on + strand, within Pf6N2E2_1286 at 1516.921 kb on + strand, within Pf6N2E2_1286 at 1516.922 kb on - strand, within Pf6N2E2_1286 at 1516.922 kb on - strand, within Pf6N2E2_1286 at 1517.122 kb on + strand, within Pf6N2E2_1286 at 1517.122 kb on + strand, within Pf6N2E2_1286
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 1,513,472 + Pf6N2E2_1283 0.39 +1.0 1,513,472 + Pf6N2E2_1283 0.39 +0.3 1,513,473 - Pf6N2E2_1283 0.40 -0.1 1,513,473 - Pf6N2E2_1283 0.40 +1.1 1,514,002 + Pf6N2E2_1284 0.21 -0.4 1,514,328 + +0.5 1,514,508 - -0.2 1,514,508 - -0.7 1,514,634 + Pf6N2E2_1285 0.16 +0.9 1,514,635 - Pf6N2E2_1285 0.16 -0.7 1,514,635 - Pf6N2E2_1285 0.16 +0.5 1,514,635 - Pf6N2E2_1285 0.16 +1.6 1,514,635 - Pf6N2E2_1285 0.16 -0.6 1,514,843 + Pf6N2E2_1285 0.27 +0.2 1,514,843 + Pf6N2E2_1285 0.27 -0.3 1,514,844 - Pf6N2E2_1285 0.27 +1.6 1,514,844 - Pf6N2E2_1285 0.27 +1.1 1,514,845 + Pf6N2E2_1285 0.28 -0.5 1,514,846 - Pf6N2E2_1285 0.28 +1.2 1,515,122 + Pf6N2E2_1285 0.43 +0.2 1,515,329 + Pf6N2E2_1285 0.54 +0.8 1,515,531 + Pf6N2E2_1285 0.66 +1.6 1,515,719 + Pf6N2E2_1285 0.76 -0.6 1,515,720 - Pf6N2E2_1285 0.76 -1.0 1,515,720 - Pf6N2E2_1285 0.76 +0.5 1,515,791 + Pf6N2E2_1285 0.80 +1.3 1,515,791 + Pf6N2E2_1285 0.80 +0.8 1,515,791 + Pf6N2E2_1285 0.80 +1.6 1,515,791 + Pf6N2E2_1285 0.80 +0.3 1,515,791 + Pf6N2E2_1285 0.80 +0.6 1,515,792 - Pf6N2E2_1285 0.80 -0.4 1,516,123 - +0.6 1,516,138 + -0.8 1,516,138 + -0.9 1,516,379 - Pf6N2E2_1286 0.13 +0.4 1,516,399 + Pf6N2E2_1286 0.14 -1.1 1,516,399 + Pf6N2E2_1286 0.14 -0.5 1,516,606 + Pf6N2E2_1286 0.27 -1.6 1,516,768 + Pf6N2E2_1286 0.37 -0.2 1,516,789 + Pf6N2E2_1286 0.39 +0.2 1,516,903 + Pf6N2E2_1286 0.46 +0.3 1,516,921 + Pf6N2E2_1286 0.47 +0.9 1,516,922 - Pf6N2E2_1286 0.47 -0.2 1,516,922 - Pf6N2E2_1286 0.47 +0.8 1,517,122 + Pf6N2E2_1286 0.60 -1.8 1,517,122 + Pf6N2E2_1286 0.60 -1.4
Or see this region's nucleotide sequence