Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5268 and Pf6N2E2_5269 are separated by 175 nucleotides Pf6N2E2_5269 and Pf6N2E2_5270 are separated by 298 nucleotides Pf6N2E2_5270 and Pf6N2E2_5271 overlap by 8 nucleotides
Pf6N2E2_5268 - Thioredoxin reductase (EC 1.8.1.9), at 6,062,455 to 6,063,417
_5268
Pf6N2E2_5269 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 6,063,593 to 6,064,843
_5269
Pf6N2E2_5270 - Type III effector HopPmaJ, at 6,065,142 to 6,065,480
_5270
Pf6N2E2_5271 - FIG00953380: hypothetical protein, at 6,065,473 to 6,065,769
_5271
Position (kb)
6063
6064
6065 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6063.456 kb on + strand at 6063.457 kb on - strand at 6063.472 kb on - strand at 6063.594 kb on + strand at 6063.594 kb on + strand at 6063.595 kb on - strand at 6063.595 kb on - strand at 6063.625 kb on + strand at 6063.979 kb on - strand, within Pf6N2E2_5269 at 6063.979 kb on - strand, within Pf6N2E2_5269 at 6063.979 kb on - strand, within Pf6N2E2_5269 at 6063.979 kb on - strand, within Pf6N2E2_5269 at 6064.070 kb on - strand, within Pf6N2E2_5269 at 6064.166 kb on + strand, within Pf6N2E2_5269 at 6064.166 kb on + strand, within Pf6N2E2_5269 at 6064.166 kb on + strand, within Pf6N2E2_5269 at 6064.167 kb on - strand, within Pf6N2E2_5269 at 6064.167 kb on - strand, within Pf6N2E2_5269 at 6064.167 kb on - strand, within Pf6N2E2_5269 at 6064.167 kb on - strand, within Pf6N2E2_5269 at 6064.167 kb on - strand, within Pf6N2E2_5269 at 6064.167 kb on - strand, within Pf6N2E2_5269 at 6064.167 kb on - strand, within Pf6N2E2_5269 at 6064.371 kb on + strand, within Pf6N2E2_5269 at 6064.371 kb on + strand, within Pf6N2E2_5269 at 6064.371 kb on + strand, within Pf6N2E2_5269 at 6064.371 kb on + strand, within Pf6N2E2_5269 at 6064.371 kb on + strand, within Pf6N2E2_5269 at 6064.371 kb on + strand, within Pf6N2E2_5269 at 6064.388 kb on + strand, within Pf6N2E2_5269 at 6064.388 kb on + strand, within Pf6N2E2_5269 at 6064.407 kb on + strand, within Pf6N2E2_5269 at 6064.407 kb on + strand, within Pf6N2E2_5269 at 6064.407 kb on + strand, within Pf6N2E2_5269 at 6064.408 kb on - strand, within Pf6N2E2_5269 at 6064.408 kb on - strand, within Pf6N2E2_5269 at 6064.408 kb on - strand, within Pf6N2E2_5269 at 6064.555 kb on + strand, within Pf6N2E2_5269 at 6064.556 kb on - strand, within Pf6N2E2_5269 at 6064.614 kb on + strand, within Pf6N2E2_5269 at 6064.614 kb on + strand, within Pf6N2E2_5269 at 6064.614 kb on + strand, within Pf6N2E2_5269 at 6064.615 kb on - strand, within Pf6N2E2_5269 at 6064.615 kb on - strand, within Pf6N2E2_5269 at 6064.743 kb on + strand at 6064.743 kb on + strand at 6064.744 kb on - strand at 6064.813 kb on - strand at 6064.878 kb on - strand at 6064.881 kb on + strand at 6064.881 kb on + strand at 6064.881 kb on + strand at 6064.881 kb on + strand at 6064.882 kb on - strand at 6064.882 kb on - strand at 6064.882 kb on - strand at 6064.914 kb on + strand at 6064.914 kb on + strand at 6064.934 kb on + strand at 6064.934 kb on + strand at 6064.934 kb on + strand at 6064.935 kb on - strand at 6064.935 kb on - strand at 6064.935 kb on - strand at 6065.390 kb on - strand, within Pf6N2E2_5270 at 6065.448 kb on - strand at 6065.448 kb on - strand at 6065.465 kb on + strand at 6065.466 kb on - strand at 6065.466 kb on - strand at 6065.535 kb on - strand, within Pf6N2E2_5271 at 6065.555 kb on + strand, within Pf6N2E2_5271 at 6065.567 kb on + strand, within Pf6N2E2_5271 at 6065.631 kb on - strand, within Pf6N2E2_5271 at 6065.631 kb on - strand, within Pf6N2E2_5271 at 6065.631 kb on - strand, within Pf6N2E2_5271
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 6,063,456 + +0.9 6,063,457 - -0.1 6,063,472 - -0.9 6,063,594 + -0.2 6,063,594 + -0.3 6,063,595 - +0.0 6,063,595 - +0.5 6,063,625 + -0.4 6,063,979 - Pf6N2E2_5269 0.31 -0.0 6,063,979 - Pf6N2E2_5269 0.31 -0.7 6,063,979 - Pf6N2E2_5269 0.31 -0.2 6,063,979 - Pf6N2E2_5269 0.31 +1.3 6,064,070 - Pf6N2E2_5269 0.38 +0.1 6,064,166 + Pf6N2E2_5269 0.46 +0.1 6,064,166 + Pf6N2E2_5269 0.46 -0.4 6,064,166 + Pf6N2E2_5269 0.46 +0.4 6,064,167 - Pf6N2E2_5269 0.46 -0.3 6,064,167 - Pf6N2E2_5269 0.46 -0.4 6,064,167 - Pf6N2E2_5269 0.46 +1.5 6,064,167 - Pf6N2E2_5269 0.46 +2.1 6,064,167 - Pf6N2E2_5269 0.46 +0.6 6,064,167 - Pf6N2E2_5269 0.46 -0.3 6,064,167 - Pf6N2E2_5269 0.46 -2.4 6,064,371 + Pf6N2E2_5269 0.62 -0.0 6,064,371 + Pf6N2E2_5269 0.62 +0.2 6,064,371 + Pf6N2E2_5269 0.62 +0.1 6,064,371 + Pf6N2E2_5269 0.62 -0.1 6,064,371 + Pf6N2E2_5269 0.62 -0.2 6,064,371 + Pf6N2E2_5269 0.62 +1.4 6,064,388 + Pf6N2E2_5269 0.64 -1.6 6,064,388 + Pf6N2E2_5269 0.64 +0.1 6,064,407 + Pf6N2E2_5269 0.65 +0.2 6,064,407 + Pf6N2E2_5269 0.65 -0.4 6,064,407 + Pf6N2E2_5269 0.65 -1.0 6,064,408 - Pf6N2E2_5269 0.65 -0.7 6,064,408 - Pf6N2E2_5269 0.65 +1.1 6,064,408 - Pf6N2E2_5269 0.65 +0.5 6,064,555 + Pf6N2E2_5269 0.77 -0.2 6,064,556 - Pf6N2E2_5269 0.77 +0.9 6,064,614 + Pf6N2E2_5269 0.82 +0.3 6,064,614 + Pf6N2E2_5269 0.82 +0.2 6,064,614 + Pf6N2E2_5269 0.82 +0.4 6,064,615 - Pf6N2E2_5269 0.82 -0.3 6,064,615 - Pf6N2E2_5269 0.82 -1.0 6,064,743 + +0.2 6,064,743 + +0.0 6,064,744 - +1.1 6,064,813 - +1.1 6,064,878 - -1.6 6,064,881 + +0.2 6,064,881 + +0.5 6,064,881 + -1.0 6,064,881 + -0.1 6,064,882 - +2.1 6,064,882 - +0.7 6,064,882 - -0.1 6,064,914 + -0.3 6,064,914 + +0.1 6,064,934 + -1.2 6,064,934 + +0.8 6,064,934 + -0.2 6,064,935 - +0.4 6,064,935 - +0.5 6,064,935 - +0.2 6,065,390 - Pf6N2E2_5270 0.73 +0.5 6,065,448 - +0.5 6,065,448 - -1.1 6,065,465 + -0.2 6,065,466 - +1.0 6,065,466 - -0.2 6,065,535 - Pf6N2E2_5271 0.21 +0.2 6,065,555 + Pf6N2E2_5271 0.28 +0.8 6,065,567 + Pf6N2E2_5271 0.32 -0.3 6,065,631 - Pf6N2E2_5271 0.53 -0.1 6,065,631 - Pf6N2E2_5271 0.53 +0.1 6,065,631 - Pf6N2E2_5271 0.53 +0.4
Or see this region's nucleotide sequence