Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4597 and Pf6N2E2_4598 are separated by 309 nucleotides Pf6N2E2_4598 and Pf6N2E2_4599 are separated by 5 nucleotides Pf6N2E2_4599 and Pf6N2E2_4600 are separated by 10 nucleotides
Pf6N2E2_4597 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 5,323,994 to 5,325,085
_4597
Pf6N2E2_4598 - COG1872, at 5,325,395 to 5,325,688
_4598
Pf6N2E2_4599 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 5,325,694 to 5,326,284
_4599
Pf6N2E2_4600 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 5,326,295 to 5,327,113
_4600
Position (kb) 5325
5326 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5324.433 kb on + strand, within Pf6N2E2_4597 at 5324.433 kb on + strand, within Pf6N2E2_4597 at 5324.433 kb on + strand, within Pf6N2E2_4597 at 5324.434 kb on - strand, within Pf6N2E2_4597 at 5324.434 kb on - strand, within Pf6N2E2_4597 at 5324.434 kb on - strand, within Pf6N2E2_4597 at 5325.009 kb on + strand at 5325.018 kb on + strand at 5325.018 kb on + strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.030 kb on + strand at 5325.031 kb on - strand at 5325.031 kb on - strand at 5325.031 kb on - strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.162 kb on - strand at 5325.167 kb on + strand at 5325.168 kb on - strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.318 kb on + strand at 5325.318 kb on + strand at 5325.318 kb on + strand at 5325.318 kb on + strand at 5325.319 kb on - strand at 5325.319 kb on - strand at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.681 kb on + strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.828 kb on + strand, within Pf6N2E2_4599 at 5325.828 kb on + strand, within Pf6N2E2_4599 at 5325.829 kb on - strand, within Pf6N2E2_4599 at 5326.020 kb on - strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.296 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 5,324,433 + Pf6N2E2_4597 0.40 -4.4 5,324,433 + Pf6N2E2_4597 0.40 -3.1 5,324,433 + Pf6N2E2_4597 0.40 -3.4 5,324,434 - Pf6N2E2_4597 0.40 -3.5 5,324,434 - Pf6N2E2_4597 0.40 -3.6 5,324,434 - Pf6N2E2_4597 0.40 -2.0 5,325,009 + -1.0 5,325,018 + -3.4 5,325,018 + +0.6 5,325,019 - -3.0 5,325,019 - -3.3 5,325,019 - -2.9 5,325,019 - -2.7 5,325,019 - -4.0 5,325,030 + -3.3 5,325,031 - -4.2 5,325,031 - -4.0 5,325,031 - -2.0 5,325,161 + -3.5 5,325,161 + -1.6 5,325,161 + -3.8 5,325,161 + -3.4 5,325,161 + -2.1 5,325,161 + -3.1 5,325,161 + -3.5 5,325,161 + -2.9 5,325,161 + -3.1 5,325,162 - -2.5 5,325,167 + -3.2 5,325,168 - +1.6 5,325,314 + +0.6 5,325,314 + +0.3 5,325,314 + +0.4 5,325,314 + -0.2 5,325,314 + +0.4 5,325,314 + -0.4 5,325,314 + +0.4 5,325,315 - -1.1 5,325,315 - -0.6 5,325,315 - -0.8 5,325,315 - -1.5 5,325,315 - +0.8 5,325,315 - +0.0 5,325,315 - -0.1 5,325,315 - +1.7 5,325,315 - -2.5 5,325,318 + -0.4 5,325,318 + +0.2 5,325,318 + +0.9 5,325,318 + -0.1 5,325,319 - -0.8 5,325,319 - -0.9 5,325,446 + Pf6N2E2_4598 0.17 +0.3 5,325,446 + Pf6N2E2_4598 0.17 +0.8 5,325,446 + Pf6N2E2_4598 0.17 +1.3 5,325,446 + Pf6N2E2_4598 0.17 -0.5 5,325,446 + Pf6N2E2_4598 0.17 +0.7 5,325,447 - Pf6N2E2_4598 0.18 +1.3 5,325,447 - Pf6N2E2_4598 0.18 +0.6 5,325,447 - Pf6N2E2_4598 0.18 +1.3 5,325,447 - Pf6N2E2_4598 0.18 -0.3 5,325,447 - Pf6N2E2_4598 0.18 -0.9 5,325,681 + -0.5 5,325,682 - -0.3 5,325,682 - +1.3 5,325,682 - -0.0 5,325,682 - +0.3 5,325,682 - +0.3 5,325,682 - +0.3 5,325,682 - +0.0 5,325,828 + Pf6N2E2_4599 0.23 -0.2 5,325,828 + Pf6N2E2_4599 0.23 +0.6 5,325,829 - Pf6N2E2_4599 0.23 +0.5 5,326,020 - Pf6N2E2_4599 0.55 +1.6 5,326,175 + Pf6N2E2_4599 0.81 +1.3 5,326,175 + Pf6N2E2_4599 0.81 +1.3 5,326,175 + Pf6N2E2_4599 0.81 -1.2 5,326,175 + Pf6N2E2_4599 0.81 -0.1 5,326,288 + +2.4 5,326,288 + -0.1 5,326,288 + +1.2 5,326,288 + -0.2 5,326,289 - +1.1 5,326,289 - +1.3 5,326,289 - -2.0 5,326,289 - -0.8 5,326,296 + +0.6
Or see this region's nucleotide sequence