Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4131 and Pf6N2E2_4132 are separated by 195 nucleotides Pf6N2E2_4132 and Pf6N2E2_4133 are separated by 15 nucleotides
Pf6N2E2_4131 - Sulfate permease, at 4,809,452 to 4,811,020
_4131
Pf6N2E2_4132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 4,811,216 to 4,812,136
_4132
Pf6N2E2_4133 - Choline-sulfatase (EC 3.1.6.6), at 4,812,152 to 4,813,684
_4133
Position (kb)
4811
4812
4813 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4810.472 kb on - strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.713 kb on + strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.714 kb on - strand, within Pf6N2E2_4131 at 4810.755 kb on + strand, within Pf6N2E2_4131 at 4810.755 kb on + strand, within Pf6N2E2_4131 at 4810.756 kb on - strand, within Pf6N2E2_4131 at 4810.756 kb on - strand, within Pf6N2E2_4131 at 4810.848 kb on + strand, within Pf6N2E2_4131 at 4810.848 kb on + strand, within Pf6N2E2_4131 at 4810.848 kb on + strand, within Pf6N2E2_4131 at 4810.849 kb on - strand, within Pf6N2E2_4131 at 4810.849 kb on - strand, within Pf6N2E2_4131 at 4810.849 kb on - strand, within Pf6N2E2_4131 at 4810.849 kb on - strand, within Pf6N2E2_4131 at 4810.887 kb on + strand at 4811.052 kb on + strand at 4811.052 kb on + strand at 4811.052 kb on + strand at 4811.052 kb on + strand at 4811.053 kb on - strand at 4811.053 kb on - strand at 4811.053 kb on - strand at 4811.138 kb on + strand at 4811.217 kb on + strand at 4811.217 kb on + strand at 4811.218 kb on - strand at 4811.440 kb on - strand, within Pf6N2E2_4132 at 4811.458 kb on + strand, within Pf6N2E2_4132 at 4811.460 kb on + strand, within Pf6N2E2_4132 at 4811.460 kb on + strand, within Pf6N2E2_4132 at 4811.460 kb on + strand, within Pf6N2E2_4132 at 4811.460 kb on + strand, within Pf6N2E2_4132 at 4811.461 kb on - strand, within Pf6N2E2_4132 at 4811.461 kb on - strand, within Pf6N2E2_4132 at 4811.461 kb on - strand, within Pf6N2E2_4132 at 4811.484 kb on + strand, within Pf6N2E2_4132 at 4811.484 kb on + strand, within Pf6N2E2_4132 at 4811.485 kb on - strand, within Pf6N2E2_4132 at 4811.505 kb on + strand, within Pf6N2E2_4132 at 4811.505 kb on + strand, within Pf6N2E2_4132 at 4811.506 kb on - strand, within Pf6N2E2_4132 at 4811.506 kb on - strand, within Pf6N2E2_4132 at 4811.506 kb on - strand, within Pf6N2E2_4132 at 4811.709 kb on + strand, within Pf6N2E2_4132 at 4811.709 kb on + strand, within Pf6N2E2_4132 at 4811.709 kb on + strand, within Pf6N2E2_4132 at 4811.709 kb on + strand, within Pf6N2E2_4132 at 4811.710 kb on - strand, within Pf6N2E2_4132 at 4811.710 kb on - strand, within Pf6N2E2_4132 at 4811.758 kb on + strand, within Pf6N2E2_4132 at 4811.758 kb on + strand, within Pf6N2E2_4132 at 4811.759 kb on - strand, within Pf6N2E2_4132 at 4811.781 kb on + strand, within Pf6N2E2_4132 at 4811.781 kb on + strand, within Pf6N2E2_4132 at 4811.781 kb on + strand, within Pf6N2E2_4132 at 4811.781 kb on + strand, within Pf6N2E2_4132 at 4811.781 kb on + strand, within Pf6N2E2_4132 at 4811.781 kb on + strand, within Pf6N2E2_4132 at 4811.782 kb on - strand, within Pf6N2E2_4132 at 4811.782 kb on - strand, within Pf6N2E2_4132 at 4811.782 kb on - strand, within Pf6N2E2_4132 at 4811.865 kb on + strand, within Pf6N2E2_4132 at 4811.973 kb on + strand, within Pf6N2E2_4132 at 4811.974 kb on - strand, within Pf6N2E2_4132 at 4811.974 kb on - strand, within Pf6N2E2_4132 at 4812.116 kb on + strand at 4812.116 kb on + strand at 4812.117 kb on - strand at 4812.148 kb on + strand at 4812.148 kb on + strand at 4812.148 kb on + strand at 4812.148 kb on + strand at 4812.149 kb on - strand at 4812.149 kb on - strand at 4812.149 kb on - strand at 4812.149 kb on - strand at 4812.149 kb on - strand at 4812.149 kb on - strand at 4812.217 kb on + strand at 4812.218 kb on - strand at 4812.225 kb on + strand at 4812.225 kb on + strand at 4812.225 kb on + strand at 4812.225 kb on + strand at 4812.225 kb on + strand at 4812.225 kb on + strand at 4812.225 kb on + strand at 4812.226 kb on - strand at 4812.226 kb on - strand at 4812.226 kb on - strand at 4812.226 kb on - strand at 4812.226 kb on - strand at 4812.226 kb on - strand at 4812.226 kb on - strand at 4812.226 kb on - strand at 4812.226 kb on - strand at 4812.226 kb on - strand at 4812.480 kb on + strand, within Pf6N2E2_4133 at 4812.480 kb on + strand, within Pf6N2E2_4133 at 4812.480 kb on + strand, within Pf6N2E2_4133 at 4812.481 kb on - strand, within Pf6N2E2_4133 at 4812.481 kb on - strand, within Pf6N2E2_4133 at 4812.481 kb on - strand, within Pf6N2E2_4133 at 4812.543 kb on + strand, within Pf6N2E2_4133 at 4812.543 kb on + strand, within Pf6N2E2_4133 at 4812.543 kb on + strand, within Pf6N2E2_4133 at 4812.543 kb on + strand, within Pf6N2E2_4133 at 4812.543 kb on + strand, within Pf6N2E2_4133 at 4812.543 kb on + strand, within Pf6N2E2_4133 at 4812.543 kb on + strand, within Pf6N2E2_4133 at 4812.543 kb on + strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.544 kb on - strand, within Pf6N2E2_4133 at 4812.768 kb on + strand, within Pf6N2E2_4133 at 4812.836 kb on + strand, within Pf6N2E2_4133 at 4812.837 kb on - strand, within Pf6N2E2_4133 at 4812.888 kb on + strand, within Pf6N2E2_4133 at 4812.888 kb on + strand, within Pf6N2E2_4133 at 4812.888 kb on + strand, within Pf6N2E2_4133 at 4812.888 kb on + strand, within Pf6N2E2_4133 at 4812.888 kb on + strand, within Pf6N2E2_4133 at 4812.889 kb on - strand, within Pf6N2E2_4133 at 4812.889 kb on - strand, within Pf6N2E2_4133 at 4812.889 kb on - strand, within Pf6N2E2_4133 at 4812.889 kb on - strand, within Pf6N2E2_4133 at 4812.889 kb on - strand, within Pf6N2E2_4133 at 4812.889 kb on - strand, within Pf6N2E2_4133 at 4812.889 kb on - strand, within Pf6N2E2_4133 at 4812.931 kb on + strand, within Pf6N2E2_4133 at 4812.932 kb on - strand, within Pf6N2E2_4133 at 4812.963 kb on + strand, within Pf6N2E2_4133 at 4812.963 kb on + strand, within Pf6N2E2_4133 at 4812.963 kb on + strand, within Pf6N2E2_4133 at 4812.963 kb on + strand, within Pf6N2E2_4133 at 4812.963 kb on + strand, within Pf6N2E2_4133 at 4812.963 kb on + strand, within Pf6N2E2_4133 at 4812.963 kb on + strand, within Pf6N2E2_4133 at 4812.963 kb on + strand, within Pf6N2E2_4133 at 4812.964 kb on - strand, within Pf6N2E2_4133 at 4812.964 kb on - strand, within Pf6N2E2_4133 at 4813.044 kb on + strand, within Pf6N2E2_4133 at 4813.044 kb on + strand, within Pf6N2E2_4133 at 4813.045 kb on - strand, within Pf6N2E2_4133 at 4813.045 kb on - strand, within Pf6N2E2_4133
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 4,810,472 - Pf6N2E2_4131 0.65 +0.6 4,810,713 + Pf6N2E2_4131 0.80 +0.1 4,810,713 + Pf6N2E2_4131 0.80 +0.1 4,810,713 + Pf6N2E2_4131 0.80 +0.4 4,810,713 + Pf6N2E2_4131 0.80 -0.9 4,810,713 + Pf6N2E2_4131 0.80 -0.1 4,810,713 + Pf6N2E2_4131 0.80 -0.9 4,810,713 + Pf6N2E2_4131 0.80 +0.4 4,810,713 + Pf6N2E2_4131 0.80 -0.8 4,810,713 + Pf6N2E2_4131 0.80 -0.1 4,810,713 + Pf6N2E2_4131 0.80 -0.1 4,810,713 + Pf6N2E2_4131 0.80 -0.4 4,810,713 + Pf6N2E2_4131 0.80 +0.0 4,810,714 - Pf6N2E2_4131 0.80 -0.1 4,810,714 - Pf6N2E2_4131 0.80 +0.3 4,810,714 - Pf6N2E2_4131 0.80 -0.1 4,810,714 - Pf6N2E2_4131 0.80 -1.0 4,810,714 - Pf6N2E2_4131 0.80 +0.1 4,810,714 - Pf6N2E2_4131 0.80 -1.4 4,810,714 - Pf6N2E2_4131 0.80 +0.9 4,810,714 - Pf6N2E2_4131 0.80 +0.8 4,810,714 - Pf6N2E2_4131 0.80 -0.0 4,810,714 - Pf6N2E2_4131 0.80 -0.1 4,810,755 + Pf6N2E2_4131 0.83 +1.2 4,810,755 + Pf6N2E2_4131 0.83 -0.9 4,810,756 - Pf6N2E2_4131 0.83 -0.9 4,810,756 - Pf6N2E2_4131 0.83 +0.5 4,810,848 + Pf6N2E2_4131 0.89 +0.2 4,810,848 + Pf6N2E2_4131 0.89 +0.4 4,810,848 + Pf6N2E2_4131 0.89 -0.2 4,810,849 - Pf6N2E2_4131 0.89 +0.2 4,810,849 - Pf6N2E2_4131 0.89 +0.1 4,810,849 - Pf6N2E2_4131 0.89 +0.0 4,810,849 - Pf6N2E2_4131 0.89 -0.7 4,810,887 + +0.4 4,811,052 + -0.1 4,811,052 + +0.2 4,811,052 + -0.2 4,811,052 + +0.4 4,811,053 - +0.1 4,811,053 - -0.2 4,811,053 - -0.1 4,811,138 + +1.2 4,811,217 + +1.2 4,811,217 + +1.4 4,811,218 - +0.0 4,811,440 - Pf6N2E2_4132 0.24 +0.2 4,811,458 + Pf6N2E2_4132 0.26 -0.4 4,811,460 + Pf6N2E2_4132 0.26 -1.6 4,811,460 + Pf6N2E2_4132 0.26 -0.5 4,811,460 + Pf6N2E2_4132 0.26 -0.4 4,811,460 + Pf6N2E2_4132 0.26 -0.8 4,811,461 - Pf6N2E2_4132 0.27 +0.3 4,811,461 - Pf6N2E2_4132 0.27 +2.1 4,811,461 - Pf6N2E2_4132 0.27 +0.3 4,811,484 + Pf6N2E2_4132 0.29 +0.7 4,811,484 + Pf6N2E2_4132 0.29 -0.9 4,811,485 - Pf6N2E2_4132 0.29 -0.2 4,811,505 + Pf6N2E2_4132 0.31 -0.3 4,811,505 + Pf6N2E2_4132 0.31 +1.0 4,811,506 - Pf6N2E2_4132 0.31 +0.4 4,811,506 - Pf6N2E2_4132 0.31 -0.8 4,811,506 - Pf6N2E2_4132 0.31 +0.1 4,811,709 + Pf6N2E2_4132 0.54 -0.4 4,811,709 + Pf6N2E2_4132 0.54 -0.4 4,811,709 + Pf6N2E2_4132 0.54 +0.5 4,811,709 + Pf6N2E2_4132 0.54 +0.3 4,811,710 - Pf6N2E2_4132 0.54 +0.4 4,811,710 - Pf6N2E2_4132 0.54 -1.0 4,811,758 + Pf6N2E2_4132 0.59 -0.9 4,811,758 + Pf6N2E2_4132 0.59 +0.2 4,811,759 - Pf6N2E2_4132 0.59 -0.4 4,811,781 + Pf6N2E2_4132 0.61 -0.8 4,811,781 + Pf6N2E2_4132 0.61 -0.4 4,811,781 + Pf6N2E2_4132 0.61 +0.3 4,811,781 + Pf6N2E2_4132 0.61 -0.7 4,811,781 + Pf6N2E2_4132 0.61 -0.5 4,811,781 + Pf6N2E2_4132 0.61 -0.1 4,811,782 - Pf6N2E2_4132 0.61 -0.7 4,811,782 - Pf6N2E2_4132 0.61 +1.3 4,811,782 - Pf6N2E2_4132 0.61 -0.1 4,811,865 + Pf6N2E2_4132 0.70 +0.1 4,811,973 + Pf6N2E2_4132 0.82 -0.2 4,811,974 - Pf6N2E2_4132 0.82 -0.1 4,811,974 - Pf6N2E2_4132 0.82 -0.3 4,812,116 + +0.3 4,812,116 + -0.3 4,812,117 - +0.1 4,812,148 + -0.3 4,812,148 + +0.8 4,812,148 + +0.4 4,812,148 + -0.3 4,812,149 - -0.9 4,812,149 - -0.6 4,812,149 - -2.5 4,812,149 - -0.2 4,812,149 - -0.8 4,812,149 - -0.1 4,812,217 + +0.8 4,812,218 - -0.1 4,812,225 + +0.5 4,812,225 + +1.2 4,812,225 + +0.4 4,812,225 + +0.3 4,812,225 + +0.5 4,812,225 + -1.1 4,812,225 + -0.5 4,812,226 - +1.3 4,812,226 - -0.1 4,812,226 - -0.2 4,812,226 - -0.5 4,812,226 - +0.4 4,812,226 - +0.3 4,812,226 - -0.3 4,812,226 - -0.5 4,812,226 - +0.3 4,812,226 - +1.2 4,812,480 + Pf6N2E2_4133 0.21 -1.9 4,812,480 + Pf6N2E2_4133 0.21 -2.1 4,812,480 + Pf6N2E2_4133 0.21 -0.3 4,812,481 - Pf6N2E2_4133 0.21 +0.2 4,812,481 - Pf6N2E2_4133 0.21 +0.2 4,812,481 - Pf6N2E2_4133 0.21 -0.8 4,812,543 + Pf6N2E2_4133 0.26 +0.6 4,812,543 + Pf6N2E2_4133 0.26 -0.3 4,812,543 + Pf6N2E2_4133 0.26 -1.2 4,812,543 + Pf6N2E2_4133 0.26 +0.1 4,812,543 + Pf6N2E2_4133 0.26 -0.0 4,812,543 + Pf6N2E2_4133 0.26 -0.1 4,812,543 + Pf6N2E2_4133 0.26 -0.8 4,812,543 + Pf6N2E2_4133 0.26 +0.5 4,812,544 - Pf6N2E2_4133 0.26 -0.0 4,812,544 - Pf6N2E2_4133 0.26 -0.4 4,812,544 - Pf6N2E2_4133 0.26 +1.3 4,812,544 - Pf6N2E2_4133 0.26 -0.7 4,812,544 - Pf6N2E2_4133 0.26 -1.0 4,812,544 - Pf6N2E2_4133 0.26 +1.0 4,812,544 - Pf6N2E2_4133 0.26 +0.5 4,812,544 - Pf6N2E2_4133 0.26 +0.0 4,812,544 - Pf6N2E2_4133 0.26 -3.6 4,812,544 - Pf6N2E2_4133 0.26 +0.5 4,812,544 - Pf6N2E2_4133 0.26 -0.9 4,812,544 - Pf6N2E2_4133 0.26 -0.8 4,812,768 + Pf6N2E2_4133 0.40 +0.4 4,812,836 + Pf6N2E2_4133 0.45 -1.6 4,812,837 - Pf6N2E2_4133 0.45 +1.0 4,812,888 + Pf6N2E2_4133 0.48 +0.4 4,812,888 + Pf6N2E2_4133 0.48 -0.9 4,812,888 + Pf6N2E2_4133 0.48 +0.1 4,812,888 + Pf6N2E2_4133 0.48 +0.3 4,812,888 + Pf6N2E2_4133 0.48 +1.4 4,812,889 - Pf6N2E2_4133 0.48 -0.3 4,812,889 - Pf6N2E2_4133 0.48 +0.5 4,812,889 - Pf6N2E2_4133 0.48 +0.7 4,812,889 - Pf6N2E2_4133 0.48 +0.2 4,812,889 - Pf6N2E2_4133 0.48 +0.1 4,812,889 - Pf6N2E2_4133 0.48 +0.6 4,812,889 - Pf6N2E2_4133 0.48 -0.6 4,812,931 + Pf6N2E2_4133 0.51 +0.8 4,812,932 - Pf6N2E2_4133 0.51 +0.1 4,812,963 + Pf6N2E2_4133 0.53 -0.8 4,812,963 + Pf6N2E2_4133 0.53 -0.3 4,812,963 + Pf6N2E2_4133 0.53 -1.8 4,812,963 + Pf6N2E2_4133 0.53 -0.7 4,812,963 + Pf6N2E2_4133 0.53 +0.4 4,812,963 + Pf6N2E2_4133 0.53 +0.1 4,812,963 + Pf6N2E2_4133 0.53 -0.1 4,812,963 + Pf6N2E2_4133 0.53 +0.1 4,812,964 - Pf6N2E2_4133 0.53 -0.1 4,812,964 - Pf6N2E2_4133 0.53 +0.7 4,813,044 + Pf6N2E2_4133 0.58 +0.3 4,813,044 + Pf6N2E2_4133 0.58 +0.6 4,813,045 - Pf6N2E2_4133 0.58 +0.5 4,813,045 - Pf6N2E2_4133 0.58 +0.5
Or see this region's nucleotide sequence