Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_346 and Pf6N2E2_347 are separated by 120 nucleotides Pf6N2E2_347 and Pf6N2E2_348 are separated by 132 nucleotides Pf6N2E2_348 and Pf6N2E2_349 overlap by 4 nucleotides Pf6N2E2_349 and Pf6N2E2_350 are separated by 65 nucleotides
Pf6N2E2_346 - 3-demethylubiquinone-9 3-methyltransferase, at 412,980 to 413,459
_346
Pf6N2E2_347 - Endoribonuclease L-PSP, at 413,580 to 413,981
_347
Pf6N2E2_348 - Transcriptional regulator, LysR family, at 414,114 to 415,043
_348
Pf6N2E2_349 - hypothetical protein, at 415,040 to 415,774
_349
Pf6N2E2_350 - Gfa-like protein, at 415,840 to 416,232
_350
Position (kb) 414
415
416 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 413.219 kb on + strand, within Pf6N2E2_346 at 413.636 kb on - strand, within Pf6N2E2_347 at 413.637 kb on + strand, within Pf6N2E2_347 at 413.637 kb on + strand, within Pf6N2E2_347 at 413.637 kb on + strand, within Pf6N2E2_347 at 413.638 kb on - strand, within Pf6N2E2_347 at 413.638 kb on - strand, within Pf6N2E2_347 at 413.638 kb on - strand, within Pf6N2E2_347 at 413.638 kb on - strand, within Pf6N2E2_347 at 413.697 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.936 kb on - strand, within Pf6N2E2_347 at 413.936 kb on - strand, within Pf6N2E2_347 at 413.985 kb on + strand at 413.986 kb on - strand at 413.986 kb on - strand at 413.986 kb on - strand at 414.026 kb on + strand at 414.027 kb on - strand at 414.306 kb on + strand, within Pf6N2E2_348 at 414.306 kb on + strand, within Pf6N2E2_348 at 414.456 kb on + strand, within Pf6N2E2_348 at 414.457 kb on - strand, within Pf6N2E2_348 at 414.726 kb on + strand, within Pf6N2E2_348 at 414.727 kb on - strand, within Pf6N2E2_348 at 414.801 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 415.062 kb on + strand at 415.062 kb on + strand at 415.063 kb on - strand at 415.063 kb on - strand at 415.073 kb on + strand at 415.073 kb on + strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.082 kb on + strand at 415.083 kb on - strand at 415.083 kb on - strand at 415.347 kb on + strand, within Pf6N2E2_349 at 415.515 kb on + strand, within Pf6N2E2_349 at 415.516 kb on - strand, within Pf6N2E2_349 at 415.542 kb on - strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.772 kb on + strand at 415.773 kb on - strand at 415.806 kb on + strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.865 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 413,219 + Pf6N2E2_346 0.50 +0.5 413,636 - Pf6N2E2_347 0.14 +0.9 413,637 + Pf6N2E2_347 0.14 +0.5 413,637 + Pf6N2E2_347 0.14 -0.4 413,637 + Pf6N2E2_347 0.14 +0.7 413,638 - Pf6N2E2_347 0.14 -0.8 413,638 - Pf6N2E2_347 0.14 -0.1 413,638 - Pf6N2E2_347 0.14 +0.5 413,638 - Pf6N2E2_347 0.14 -1.7 413,697 + Pf6N2E2_347 0.29 -0.4 413,935 + Pf6N2E2_347 0.88 -1.1 413,935 + Pf6N2E2_347 0.88 -0.9 413,935 + Pf6N2E2_347 0.88 -0.8 413,935 + Pf6N2E2_347 0.88 -0.1 413,935 + Pf6N2E2_347 0.88 +1.3 413,936 - Pf6N2E2_347 0.89 +0.4 413,936 - Pf6N2E2_347 0.89 -0.4 413,985 + +1.1 413,986 - -0.5 413,986 - +0.3 413,986 - -0.3 414,026 + -0.8 414,027 - +0.6 414,306 + Pf6N2E2_348 0.21 +1.0 414,306 + Pf6N2E2_348 0.21 +0.3 414,456 + Pf6N2E2_348 0.37 +1.5 414,457 - Pf6N2E2_348 0.37 -0.1 414,726 + Pf6N2E2_348 0.66 +0.4 414,727 - Pf6N2E2_348 0.66 -0.1 414,801 + Pf6N2E2_348 0.74 -0.5 414,849 + Pf6N2E2_348 0.79 +0.7 414,849 + Pf6N2E2_348 0.79 +0.2 414,849 + Pf6N2E2_348 0.79 +2.0 414,849 + Pf6N2E2_348 0.79 +1.6 414,849 + Pf6N2E2_348 0.79 +1.5 414,850 - Pf6N2E2_348 0.79 +1.5 414,850 - Pf6N2E2_348 0.79 +0.1 414,850 - Pf6N2E2_348 0.79 +2.2 414,850 - Pf6N2E2_348 0.79 +1.3 414,927 + Pf6N2E2_348 0.87 +0.1 414,927 + Pf6N2E2_348 0.87 -0.5 415,062 + -0.8 415,062 + -0.7 415,063 - -0.8 415,063 - +0.6 415,073 + +0.6 415,073 + +1.0 415,074 - +0.4 415,074 - -1.7 415,074 - +3.3 415,082 + -0.8 415,083 - +0.3 415,083 - -0.2 415,347 + Pf6N2E2_349 0.42 +1.0 415,515 + Pf6N2E2_349 0.65 +0.6 415,516 - Pf6N2E2_349 0.65 +1.3 415,542 - Pf6N2E2_349 0.68 -0.4 415,660 + Pf6N2E2_349 0.84 -0.1 415,660 + Pf6N2E2_349 0.84 -2.0 415,772 + -1.2 415,773 - +0.1 415,806 + -0.1 415,807 - +0.4 415,807 - +2.6 415,807 - -0.8 415,807 - +0.9 415,865 + +0.2
Or see this region's nucleotide sequence