Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1172 and Pf6N2E2_1173 are separated by 281 nucleotides Pf6N2E2_1173 and Pf6N2E2_1174 are separated by 278 nucleotides Pf6N2E2_1174 and Pf6N2E2_1175 are separated by 269 nucleotides
Pf6N2E2_1172 - Short-chain dehydrogenase/reductase SDR, at 1,372,316 to 1,373,089
_1172
Pf6N2E2_1173 - hypothetical protein, at 1,373,371 to 1,373,685
_1173
Pf6N2E2_1174 - hypothetical protein, at 1,373,964 to 1,375,022
_1174
Pf6N2E2_1175 - VgrG protein, at 1,375,292 to 1,377,517
_1175
Position (kb)
1373
1374
1375
1376 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1372.971 kb on + strand, within Pf6N2E2_1172 at 1373.066 kb on - strand at 1373.066 kb on - strand at 1373.107 kb on - strand at 1373.107 kb on - strand at 1373.129 kb on + strand at 1373.130 kb on - strand at 1373.161 kb on + strand at 1373.228 kb on + strand at 1373.285 kb on - strand at 1373.288 kb on + strand at 1373.472 kb on - strand, within Pf6N2E2_1173 at 1373.472 kb on - strand, within Pf6N2E2_1173 at 1373.518 kb on + strand, within Pf6N2E2_1173 at 1373.519 kb on - strand, within Pf6N2E2_1173 at 1373.519 kb on - strand, within Pf6N2E2_1173 at 1373.725 kb on + strand at 1373.791 kb on - strand at 1373.965 kb on + strand at 1374.139 kb on + strand, within Pf6N2E2_1174 at 1374.208 kb on + strand, within Pf6N2E2_1174 at 1374.208 kb on + strand, within Pf6N2E2_1174 at 1374.234 kb on + strand, within Pf6N2E2_1174 at 1374.337 kb on + strand, within Pf6N2E2_1174 at 1374.456 kb on + strand, within Pf6N2E2_1174 at 1374.457 kb on - strand, within Pf6N2E2_1174 at 1374.469 kb on + strand, within Pf6N2E2_1174 at 1374.488 kb on + strand, within Pf6N2E2_1174 at 1374.488 kb on + strand, within Pf6N2E2_1174 at 1374.595 kb on - strand, within Pf6N2E2_1174 at 1374.681 kb on - strand, within Pf6N2E2_1174 at 1374.711 kb on - strand, within Pf6N2E2_1174 at 1374.711 kb on - strand, within Pf6N2E2_1174 at 1374.711 kb on - strand, within Pf6N2E2_1174 at 1374.711 kb on - strand, within Pf6N2E2_1174 at 1374.772 kb on - strand, within Pf6N2E2_1174 at 1374.794 kb on + strand, within Pf6N2E2_1174 at 1374.816 kb on + strand, within Pf6N2E2_1174 at 1374.817 kb on - strand, within Pf6N2E2_1174 at 1374.817 kb on - strand, within Pf6N2E2_1174 at 1374.861 kb on + strand, within Pf6N2E2_1174 at 1374.862 kb on - strand, within Pf6N2E2_1174 at 1374.862 kb on - strand, within Pf6N2E2_1174 at 1374.862 kb on - strand, within Pf6N2E2_1174 at 1374.946 kb on + strand at 1374.946 kb on + strand at 1374.947 kb on - strand at 1374.996 kb on + strand at 1374.997 kb on - strand at 1374.997 kb on - strand at 1374.997 kb on - strand at 1374.997 kb on - strand at 1375.056 kb on - strand at 1375.108 kb on + strand at 1375.136 kb on + strand at 1375.136 kb on + strand at 1375.136 kb on + strand at 1375.136 kb on + strand at 1375.167 kb on + strand at 1375.207 kb on - strand at 1375.207 kb on - strand at 1375.207 kb on - strand at 1375.281 kb on + strand at 1375.487 kb on + strand at 1375.487 kb on + strand at 1375.487 kb on + strand at 1375.727 kb on + strand, within Pf6N2E2_1175 at 1375.727 kb on + strand, within Pf6N2E2_1175 at 1375.793 kb on + strand, within Pf6N2E2_1175 at 1375.793 kb on + strand, within Pf6N2E2_1175 at 1375.793 kb on + strand, within Pf6N2E2_1175 at 1375.794 kb on - strand, within Pf6N2E2_1175 at 1375.794 kb on - strand, within Pf6N2E2_1175 at 1375.794 kb on - strand, within Pf6N2E2_1175 at 1375.794 kb on - strand, within Pf6N2E2_1175 at 1375.796 kb on + strand, within Pf6N2E2_1175 at 1375.848 kb on - strand, within Pf6N2E2_1175 at 1375.874 kb on + strand, within Pf6N2E2_1175 at 1375.875 kb on - strand, within Pf6N2E2_1175 at 1375.932 kb on - strand, within Pf6N2E2_1175 at 1375.932 kb on - strand, within Pf6N2E2_1175
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 1,372,971 + Pf6N2E2_1172 0.85 +2.1 1,373,066 - +0.4 1,373,066 - +0.8 1,373,107 - +1.2 1,373,107 - +1.1 1,373,129 + -0.3 1,373,130 - +1.2 1,373,161 + +0.5 1,373,228 + -0.2 1,373,285 - -0.2 1,373,288 + -0.8 1,373,472 - Pf6N2E2_1173 0.32 +1.1 1,373,472 - Pf6N2E2_1173 0.32 -0.5 1,373,518 + Pf6N2E2_1173 0.47 +0.1 1,373,519 - Pf6N2E2_1173 0.47 +0.5 1,373,519 - Pf6N2E2_1173 0.47 -1.7 1,373,725 + +0.3 1,373,791 - +0.8 1,373,965 + +0.2 1,374,139 + Pf6N2E2_1174 0.17 +0.5 1,374,208 + Pf6N2E2_1174 0.23 +0.8 1,374,208 + Pf6N2E2_1174 0.23 +0.8 1,374,234 + Pf6N2E2_1174 0.25 -0.8 1,374,337 + Pf6N2E2_1174 0.35 +1.6 1,374,456 + Pf6N2E2_1174 0.46 +1.0 1,374,457 - Pf6N2E2_1174 0.47 +1.1 1,374,469 + Pf6N2E2_1174 0.48 +1.5 1,374,488 + Pf6N2E2_1174 0.49 +0.2 1,374,488 + Pf6N2E2_1174 0.49 -0.6 1,374,595 - Pf6N2E2_1174 0.60 +0.2 1,374,681 - Pf6N2E2_1174 0.68 +1.0 1,374,711 - Pf6N2E2_1174 0.71 +0.4 1,374,711 - Pf6N2E2_1174 0.71 -0.2 1,374,711 - Pf6N2E2_1174 0.71 +0.6 1,374,711 - Pf6N2E2_1174 0.71 +0.6 1,374,772 - Pf6N2E2_1174 0.76 +0.7 1,374,794 + Pf6N2E2_1174 0.78 +0.4 1,374,816 + Pf6N2E2_1174 0.80 +1.4 1,374,817 - Pf6N2E2_1174 0.81 -2.1 1,374,817 - Pf6N2E2_1174 0.81 +1.1 1,374,861 + Pf6N2E2_1174 0.85 +0.2 1,374,862 - Pf6N2E2_1174 0.85 +0.8 1,374,862 - Pf6N2E2_1174 0.85 -0.1 1,374,862 - Pf6N2E2_1174 0.85 -0.2 1,374,946 + -0.7 1,374,946 + -0.9 1,374,947 - -0.1 1,374,996 + -0.4 1,374,997 - -0.9 1,374,997 - +0.5 1,374,997 - +0.3 1,374,997 - -0.5 1,375,056 - +0.0 1,375,108 + +0.3 1,375,136 + +0.5 1,375,136 + +1.0 1,375,136 + -0.8 1,375,136 + -0.2 1,375,167 + -1.4 1,375,207 - +0.2 1,375,207 - -3.2 1,375,207 - +0.4 1,375,281 + +0.2 1,375,487 + +0.2 1,375,487 + +0.1 1,375,487 + -1.9 1,375,727 + Pf6N2E2_1175 0.20 +0.7 1,375,727 + Pf6N2E2_1175 0.20 -0.9 1,375,793 + Pf6N2E2_1175 0.23 +0.8 1,375,793 + Pf6N2E2_1175 0.23 -0.3 1,375,793 + Pf6N2E2_1175 0.23 +0.5 1,375,794 - Pf6N2E2_1175 0.23 +0.1 1,375,794 - Pf6N2E2_1175 0.23 -1.4 1,375,794 - Pf6N2E2_1175 0.23 -0.5 1,375,794 - Pf6N2E2_1175 0.23 -0.5 1,375,796 + Pf6N2E2_1175 0.23 +0.8 1,375,848 - Pf6N2E2_1175 0.25 -1.6 1,375,874 + Pf6N2E2_1175 0.26 +1.0 1,375,875 - Pf6N2E2_1175 0.26 +1.7 1,375,932 - Pf6N2E2_1175 0.29 +1.7 1,375,932 - Pf6N2E2_1175 0.29 +0.5
Or see this region's nucleotide sequence