Experiment: Gly-Glu (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4224 and Pf6N2E2_4225 are separated by 181 nucleotides Pf6N2E2_4225 and Pf6N2E2_4226 are separated by 9 nucleotides Pf6N2E2_4226 and Pf6N2E2_4227 are separated by 1 nucleotides Pf6N2E2_4227 and Pf6N2E2_4228 are separated by 7 nucleotides
Pf6N2E2_4224 - Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18), at 4,913,719 to 4,914,276
_4224
Pf6N2E2_4225 - Nicotinamidase (EC 3.5.1.19), at 4,914,458 to 4,915,105
_4225
Pf6N2E2_4226 - Nicotinate phosphoribosyltransferase (EC 2.4.2.11), at 4,915,115 to 4,916,344
_4226
Pf6N2E2_4227 - NAD synthetase (EC 6.3.1.5), at 4,916,346 to 4,917,182
_4227
Pf6N2E2_4228 - Chemotactic transducer, at 4,917,190 to 4,919,079
_4228
Position (kb)
4915
4916
4917 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4914.131 kb on + strand, within Pf6N2E2_4224 at 4914.132 kb on - strand, within Pf6N2E2_4224 at 4914.132 kb on - strand, within Pf6N2E2_4224 at 4914.306 kb on - strand at 4914.422 kb on - strand at 4914.422 kb on - strand at 4914.514 kb on + strand at 4914.514 kb on + strand at 4914.515 kb on - strand at 4914.768 kb on - strand, within Pf6N2E2_4225 at 4914.773 kb on + strand, within Pf6N2E2_4225 at 4914.774 kb on - strand, within Pf6N2E2_4225 at 4914.774 kb on - strand, within Pf6N2E2_4225 at 4914.779 kb on + strand, within Pf6N2E2_4225 at 4914.779 kb on + strand, within Pf6N2E2_4225 at 4914.779 kb on + strand, within Pf6N2E2_4225 at 4914.779 kb on + strand, within Pf6N2E2_4225 at 4914.780 kb on - strand, within Pf6N2E2_4225 at 4914.780 kb on - strand, within Pf6N2E2_4225 at 4914.873 kb on - strand, within Pf6N2E2_4225 at 4915.103 kb on + strand at 4915.103 kb on + strand at 4915.103 kb on + strand at 4915.104 kb on - strand at 4915.172 kb on + strand at 4915.172 kb on + strand at 4915.172 kb on + strand at 4915.172 kb on + strand at 4915.172 kb on + strand at 4915.172 kb on + strand at 4915.173 kb on - strand at 4915.173 kb on - strand at 4915.173 kb on - strand at 4915.175 kb on + strand at 4915.175 kb on + strand at 4915.176 kb on - strand at 4915.176 kb on - strand at 4915.176 kb on - strand at 4915.176 kb on - strand at 4915.176 kb on - strand at 4915.176 kb on - strand at 4915.176 kb on - strand at 4915.176 kb on - strand at 4915.176 kb on - strand at 4915.367 kb on + strand, within Pf6N2E2_4226 at 4915.367 kb on + strand, within Pf6N2E2_4226 at 4915.421 kb on + strand, within Pf6N2E2_4226 at 4915.422 kb on - strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.425 kb on + strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.426 kb on - strand, within Pf6N2E2_4226 at 4915.544 kb on + strand, within Pf6N2E2_4226 at 4915.583 kb on + strand, within Pf6N2E2_4226 at 4915.583 kb on + strand, within Pf6N2E2_4226 at 4915.584 kb on - strand, within Pf6N2E2_4226 at 4915.702 kb on + strand, within Pf6N2E2_4226 at 4915.702 kb on + strand, within Pf6N2E2_4226 at 4915.702 kb on + strand, within Pf6N2E2_4226 at 4915.702 kb on + strand, within Pf6N2E2_4226 at 4915.702 kb on + strand, within Pf6N2E2_4226 at 4915.702 kb on + strand, within Pf6N2E2_4226 at 4915.702 kb on + strand, within Pf6N2E2_4226 at 4915.702 kb on + strand, within Pf6N2E2_4226 at 4915.703 kb on - strand, within Pf6N2E2_4226 at 4915.703 kb on - strand, within Pf6N2E2_4226 at 4915.703 kb on - strand, within Pf6N2E2_4226 at 4915.703 kb on - strand, within Pf6N2E2_4226 at 4915.703 kb on - strand, within Pf6N2E2_4226 at 4915.703 kb on - strand, within Pf6N2E2_4226 at 4915.703 kb on - strand, within Pf6N2E2_4226 at 4915.705 kb on + strand, within Pf6N2E2_4226 at 4915.745 kb on - strand, within Pf6N2E2_4226 at 4915.760 kb on + strand, within Pf6N2E2_4226 at 4915.761 kb on - strand, within Pf6N2E2_4226 at 4915.761 kb on - strand, within Pf6N2E2_4226 at 4915.878 kb on - strand, within Pf6N2E2_4226 at 4915.949 kb on + strand, within Pf6N2E2_4226 at 4915.949 kb on + strand, within Pf6N2E2_4226 at 4915.950 kb on - strand, within Pf6N2E2_4226 at 4915.964 kb on + strand, within Pf6N2E2_4226 at 4915.964 kb on + strand, within Pf6N2E2_4226 at 4915.964 kb on + strand, within Pf6N2E2_4226 at 4915.964 kb on + strand, within Pf6N2E2_4226 at 4915.964 kb on + strand, within Pf6N2E2_4226 at 4915.964 kb on + strand, within Pf6N2E2_4226 at 4915.964 kb on + strand, within Pf6N2E2_4226 at 4915.965 kb on - strand, within Pf6N2E2_4226 at 4915.965 kb on - strand, within Pf6N2E2_4226 at 4915.965 kb on - strand, within Pf6N2E2_4226 at 4916.346 kb on - strand at 4916.351 kb on + strand at 4916.352 kb on - strand at 4916.352 kb on - strand at 4916.352 kb on - strand at 4916.533 kb on + strand, within Pf6N2E2_4227 at 4916.534 kb on - strand, within Pf6N2E2_4227 at 4916.534 kb on - strand, within Pf6N2E2_4227 at 4916.571 kb on + strand, within Pf6N2E2_4227 at 4916.571 kb on + strand, within Pf6N2E2_4227 at 4916.571 kb on + strand, within Pf6N2E2_4227 at 4916.571 kb on + strand, within Pf6N2E2_4227 at 4916.571 kb on + strand, within Pf6N2E2_4227 at 4916.571 kb on + strand, within Pf6N2E2_4227 at 4916.572 kb on - strand, within Pf6N2E2_4227 at 4916.585 kb on + strand, within Pf6N2E2_4227 at 4916.586 kb on - strand, within Pf6N2E2_4227 at 4916.604 kb on + strand, within Pf6N2E2_4227 at 4916.604 kb on + strand, within Pf6N2E2_4227 at 4916.604 kb on + strand, within Pf6N2E2_4227 at 4916.802 kb on + strand, within Pf6N2E2_4227 at 4916.802 kb on + strand, within Pf6N2E2_4227 at 4916.803 kb on - strand, within Pf6N2E2_4227 at 4916.803 kb on - strand, within Pf6N2E2_4227 at 4917.043 kb on - strand, within Pf6N2E2_4227 at 4917.054 kb on + strand, within Pf6N2E2_4227 at 4917.073 kb on - strand, within Pf6N2E2_4227 at 4917.127 kb on - strand at 4917.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (N) remove 4,914,131 + Pf6N2E2_4224 0.74 +0.1 4,914,132 - Pf6N2E2_4224 0.74 +0.6 4,914,132 - Pf6N2E2_4224 0.74 -1.4 4,914,306 - -0.7 4,914,422 - -0.6 4,914,422 - -1.7 4,914,514 + -0.5 4,914,514 + -1.5 4,914,515 - +0.5 4,914,768 - Pf6N2E2_4225 0.48 -0.2 4,914,773 + Pf6N2E2_4225 0.49 -0.2 4,914,774 - Pf6N2E2_4225 0.49 +0.4 4,914,774 - Pf6N2E2_4225 0.49 +0.8 4,914,779 + Pf6N2E2_4225 0.50 -1.2 4,914,779 + Pf6N2E2_4225 0.50 -1.2 4,914,779 + Pf6N2E2_4225 0.50 -1.5 4,914,779 + Pf6N2E2_4225 0.50 -0.0 4,914,780 - Pf6N2E2_4225 0.50 +1.0 4,914,780 - Pf6N2E2_4225 0.50 +1.0 4,914,873 - Pf6N2E2_4225 0.64 +0.8 4,915,103 + -1.0 4,915,103 + -1.1 4,915,103 + -1.2 4,915,104 - +0.0 4,915,172 + +0.4 4,915,172 + +0.8 4,915,172 + -0.7 4,915,172 + -0.0 4,915,172 + -0.4 4,915,172 + -1.6 4,915,173 - +0.1 4,915,173 - -1.0 4,915,173 - +1.0 4,915,175 + +0.7 4,915,175 + +0.6 4,915,176 - +0.3 4,915,176 - +0.3 4,915,176 - +0.4 4,915,176 - -0.8 4,915,176 - -0.6 4,915,176 - -0.2 4,915,176 - -0.8 4,915,176 - +0.7 4,915,176 - +0.1 4,915,367 + Pf6N2E2_4226 0.20 +0.4 4,915,367 + Pf6N2E2_4226 0.20 +0.8 4,915,421 + Pf6N2E2_4226 0.25 -0.9 4,915,422 - Pf6N2E2_4226 0.25 +0.1 4,915,425 + Pf6N2E2_4226 0.25 +0.2 4,915,425 + Pf6N2E2_4226 0.25 -1.5 4,915,425 + Pf6N2E2_4226 0.25 -1.7 4,915,425 + Pf6N2E2_4226 0.25 +0.1 4,915,425 + Pf6N2E2_4226 0.25 +0.0 4,915,425 + Pf6N2E2_4226 0.25 -1.3 4,915,425 + Pf6N2E2_4226 0.25 -2.0 4,915,425 + Pf6N2E2_4226 0.25 +0.2 4,915,425 + Pf6N2E2_4226 0.25 +0.2 4,915,425 + Pf6N2E2_4226 0.25 -1.4 4,915,425 + Pf6N2E2_4226 0.25 -0.2 4,915,425 + Pf6N2E2_4226 0.25 -0.6 4,915,426 - Pf6N2E2_4226 0.25 +0.9 4,915,426 - Pf6N2E2_4226 0.25 -0.8 4,915,426 - Pf6N2E2_4226 0.25 +0.4 4,915,426 - Pf6N2E2_4226 0.25 +0.3 4,915,426 - Pf6N2E2_4226 0.25 -0.4 4,915,426 - Pf6N2E2_4226 0.25 +0.1 4,915,426 - Pf6N2E2_4226 0.25 +1.0 4,915,426 - Pf6N2E2_4226 0.25 -0.1 4,915,426 - Pf6N2E2_4226 0.25 +0.9 4,915,426 - Pf6N2E2_4226 0.25 -1.6 4,915,426 - Pf6N2E2_4226 0.25 -0.2 4,915,426 - Pf6N2E2_4226 0.25 +1.7 4,915,426 - Pf6N2E2_4226 0.25 -0.0 4,915,544 + Pf6N2E2_4226 0.35 +1.6 4,915,583 + Pf6N2E2_4226 0.38 -0.2 4,915,583 + Pf6N2E2_4226 0.38 -0.2 4,915,584 - Pf6N2E2_4226 0.38 -0.4 4,915,702 + Pf6N2E2_4226 0.48 -0.1 4,915,702 + Pf6N2E2_4226 0.48 -1.0 4,915,702 + Pf6N2E2_4226 0.48 -0.6 4,915,702 + Pf6N2E2_4226 0.48 +1.1 4,915,702 + Pf6N2E2_4226 0.48 -0.5 4,915,702 + Pf6N2E2_4226 0.48 +0.4 4,915,702 + Pf6N2E2_4226 0.48 +0.6 4,915,702 + Pf6N2E2_4226 0.48 +0.5 4,915,703 - Pf6N2E2_4226 0.48 -0.4 4,915,703 - Pf6N2E2_4226 0.48 +0.2 4,915,703 - Pf6N2E2_4226 0.48 +0.4 4,915,703 - Pf6N2E2_4226 0.48 +0.0 4,915,703 - Pf6N2E2_4226 0.48 +0.0 4,915,703 - Pf6N2E2_4226 0.48 +0.2 4,915,703 - Pf6N2E2_4226 0.48 -0.1 4,915,705 + Pf6N2E2_4226 0.48 +2.1 4,915,745 - Pf6N2E2_4226 0.51 -1.0 4,915,760 + Pf6N2E2_4226 0.52 +1.5 4,915,761 - Pf6N2E2_4226 0.53 -1.8 4,915,761 - Pf6N2E2_4226 0.53 -1.6 4,915,878 - Pf6N2E2_4226 0.62 +0.1 4,915,949 + Pf6N2E2_4226 0.68 -0.8 4,915,949 + Pf6N2E2_4226 0.68 -0.4 4,915,950 - Pf6N2E2_4226 0.68 -0.1 4,915,964 + Pf6N2E2_4226 0.69 -0.5 4,915,964 + Pf6N2E2_4226 0.69 -0.2 4,915,964 + Pf6N2E2_4226 0.69 +0.4 4,915,964 + Pf6N2E2_4226 0.69 +0.3 4,915,964 + Pf6N2E2_4226 0.69 +1.2 4,915,964 + Pf6N2E2_4226 0.69 +0.0 4,915,964 + Pf6N2E2_4226 0.69 -0.4 4,915,965 - Pf6N2E2_4226 0.69 +1.1 4,915,965 - Pf6N2E2_4226 0.69 -0.3 4,915,965 - Pf6N2E2_4226 0.69 +0.3 4,916,346 - +1.2 4,916,351 + -0.2 4,916,352 - +0.0 4,916,352 - +0.6 4,916,352 - +1.0 4,916,533 + Pf6N2E2_4227 0.22 -0.2 4,916,534 - Pf6N2E2_4227 0.22 -0.3 4,916,534 - Pf6N2E2_4227 0.22 -0.5 4,916,571 + Pf6N2E2_4227 0.27 -0.2 4,916,571 + Pf6N2E2_4227 0.27 -0.1 4,916,571 + Pf6N2E2_4227 0.27 +0.1 4,916,571 + Pf6N2E2_4227 0.27 -0.2 4,916,571 + Pf6N2E2_4227 0.27 +0.8 4,916,571 + Pf6N2E2_4227 0.27 -0.8 4,916,572 - Pf6N2E2_4227 0.27 +0.9 4,916,585 + Pf6N2E2_4227 0.29 +0.8 4,916,586 - Pf6N2E2_4227 0.29 +0.3 4,916,604 + Pf6N2E2_4227 0.31 -0.4 4,916,604 + Pf6N2E2_4227 0.31 +0.4 4,916,604 + Pf6N2E2_4227 0.31 +0.7 4,916,802 + Pf6N2E2_4227 0.54 -1.4 4,916,802 + Pf6N2E2_4227 0.54 +0.1 4,916,803 - Pf6N2E2_4227 0.55 +0.4 4,916,803 - Pf6N2E2_4227 0.55 +0.5 4,917,043 - Pf6N2E2_4227 0.83 -0.0 4,917,054 + Pf6N2E2_4227 0.85 -0.0 4,917,073 - Pf6N2E2_4227 0.87 +0.6 4,917,127 - -0.1 4,917,127 - +0.2
Or see this region's nucleotide sequence