Experiment: Gly-Glu (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_292 and Pf6N2E2_293 are separated by 217 nucleotides Pf6N2E2_293 and Pf6N2E2_294 are separated by 123 nucleotides Pf6N2E2_294 and Pf6N2E2_295 are separated by 57 nucleotides Pf6N2E2_295 and Pf6N2E2_296 are separated by 81 nucleotides
Pf6N2E2_292 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 360,587 to 362,857
_292
Pf6N2E2_293 - Translation initiation factor 1, at 363,075 to 363,293
_293
Pf6N2E2_294 - Arginine-tRNA-protein transferase (EC 2.3.2.8), at 363,417 to 364,124
_294
Pf6N2E2_295 - Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6), at 364,182 to 364,862
_295
Pf6N2E2_296 - Thioredoxin reductase (EC 1.8.1.9), at 364,944 to 365,903
_296
Position (kb) 363
364
365 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 362.907 kb on - strand at 362.907 kb on - strand at 363.396 kb on - strand at 363.396 kb on - strand at 363.402 kb on - strand at 363.402 kb on - strand at 363.402 kb on - strand at 363.404 kb on - strand at 363.479 kb on - strand at 363.517 kb on + strand, within Pf6N2E2_294 at 363.518 kb on - strand, within Pf6N2E2_294 at 363.518 kb on - strand, within Pf6N2E2_294 at 363.608 kb on - strand, within Pf6N2E2_294 at 363.608 kb on - strand, within Pf6N2E2_294 at 363.608 kb on - strand, within Pf6N2E2_294 at 363.616 kb on + strand, within Pf6N2E2_294 at 363.616 kb on + strand, within Pf6N2E2_294 at 363.616 kb on + strand, within Pf6N2E2_294 at 363.617 kb on - strand, within Pf6N2E2_294 at 363.692 kb on - strand, within Pf6N2E2_294 at 363.692 kb on - strand, within Pf6N2E2_294 at 363.754 kb on + strand, within Pf6N2E2_294 at 363.754 kb on + strand, within Pf6N2E2_294 at 363.754 kb on + strand, within Pf6N2E2_294 at 363.755 kb on - strand, within Pf6N2E2_294 at 363.755 kb on - strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.788 kb on - strand, within Pf6N2E2_294 at 363.797 kb on - strand, within Pf6N2E2_294 at 363.808 kb on + strand, within Pf6N2E2_294 at 363.808 kb on + strand, within Pf6N2E2_294 at 363.809 kb on - strand, within Pf6N2E2_294 at 363.809 kb on - strand, within Pf6N2E2_294 at 363.972 kb on - strand, within Pf6N2E2_294 at 364.097 kb on - strand at 364.147 kb on + strand at 364.148 kb on - strand at 364.153 kb on + strand at 364.153 kb on + strand at 364.153 kb on + strand at 364.154 kb on - strand at 364.159 kb on + strand at 364.159 kb on + strand at 364.160 kb on - strand at 364.160 kb on - strand at 364.160 kb on - strand at 364.231 kb on + strand at 364.232 kb on - strand at 364.419 kb on + strand, within Pf6N2E2_295 at 364.427 kb on - strand, within Pf6N2E2_295 at 364.544 kb on - strand, within Pf6N2E2_295 at 364.606 kb on + strand, within Pf6N2E2_295 at 364.606 kb on + strand, within Pf6N2E2_295 at 364.768 kb on - strand, within Pf6N2E2_295 at 364.867 kb on - strand at 364.878 kb on + strand at 364.878 kb on + strand at 364.878 kb on + strand at 364.878 kb on + strand at 364.878 kb on + strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.969 kb on + strand at 364.969 kb on + strand at 364.969 kb on + strand at 364.969 kb on + strand at 364.970 kb on - strand at 364.970 kb on - strand at 364.970 kb on - strand at 364.970 kb on - strand at 365.015 kb on + strand at 365.015 kb on + strand at 365.015 kb on + strand at 365.016 kb on - strand at 365.016 kb on - strand at 365.016 kb on - strand at 365.110 kb on - strand, within Pf6N2E2_296
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (N) remove 362,907 - -1.5 362,907 - +0.3 363,396 - +1.1 363,396 - -0.6 363,402 - -0.4 363,402 - -0.6 363,402 - -0.0 363,404 - +0.9 363,479 - +0.8 363,517 + Pf6N2E2_294 0.14 +1.2 363,518 - Pf6N2E2_294 0.14 -1.3 363,518 - Pf6N2E2_294 0.14 +1.3 363,608 - Pf6N2E2_294 0.27 +1.5 363,608 - Pf6N2E2_294 0.27 -0.1 363,608 - Pf6N2E2_294 0.27 +0.4 363,616 + Pf6N2E2_294 0.28 +1.0 363,616 + Pf6N2E2_294 0.28 +0.8 363,616 + Pf6N2E2_294 0.28 +0.2 363,617 - Pf6N2E2_294 0.28 +2.0 363,692 - Pf6N2E2_294 0.39 +1.4 363,692 - Pf6N2E2_294 0.39 +0.8 363,754 + Pf6N2E2_294 0.48 +1.4 363,754 + Pf6N2E2_294 0.48 +0.5 363,754 + Pf6N2E2_294 0.48 -0.0 363,755 - Pf6N2E2_294 0.48 -0.5 363,755 - Pf6N2E2_294 0.48 +0.4 363,787 + Pf6N2E2_294 0.52 +1.6 363,787 + Pf6N2E2_294 0.52 +1.3 363,787 + Pf6N2E2_294 0.52 +0.7 363,787 + Pf6N2E2_294 0.52 +2.2 363,788 - Pf6N2E2_294 0.52 +3.0 363,797 - Pf6N2E2_294 0.54 +2.3 363,808 + Pf6N2E2_294 0.55 +0.5 363,808 + Pf6N2E2_294 0.55 +0.5 363,809 - Pf6N2E2_294 0.55 +0.6 363,809 - Pf6N2E2_294 0.55 -0.3 363,972 - Pf6N2E2_294 0.78 +1.5 364,097 - +0.4 364,147 + -1.2 364,148 - +2.1 364,153 + +0.1 364,153 + +0.4 364,153 + -0.1 364,154 - +1.9 364,159 + +0.5 364,159 + -0.6 364,160 - +0.2 364,160 - +0.9 364,160 - -1.0 364,231 + +1.1 364,232 - -0.9 364,419 + Pf6N2E2_295 0.35 +0.4 364,427 - Pf6N2E2_295 0.36 +0.8 364,544 - Pf6N2E2_295 0.53 -2.0 364,606 + Pf6N2E2_295 0.62 +0.7 364,606 + Pf6N2E2_295 0.62 -0.6 364,768 - Pf6N2E2_295 0.86 +0.0 364,867 - -0.1 364,878 + -0.5 364,878 + -0.2 364,878 + -0.4 364,878 + -0.4 364,878 + +0.2 364,879 - -0.2 364,879 - +2.5 364,879 - -1.2 364,879 - +0.1 364,879 - -0.8 364,879 - -1.3 364,879 - -0.4 364,969 + +0.7 364,969 + +0.2 364,969 + -1.8 364,969 + +1.1 364,970 - +0.2 364,970 - -0.1 364,970 - +1.2 364,970 - +0.1 365,015 + +0.0 365,015 + +0.8 365,015 + -0.8 365,016 - +0.1 365,016 - -0.5 365,016 - -2.9 365,110 - Pf6N2E2_296 0.17 +0.1
Or see this region's nucleotide sequence