Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5516 and Pf6N2E2_5517 are separated by 93 nucleotides Pf6N2E2_5517 and Pf6N2E2_5518 are separated by 11 nucleotides
Pf6N2E2_5516 - Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1), at 6,335,232 to 6,336,449
_5516
Pf6N2E2_5517 - Glutathione S-transferase (EC 2.5.1.18), at 6,336,543 to 6,337,175
_5517
Pf6N2E2_5518 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein, at 6,337,187 to 6,338,116
_5518
Position (kb)
6336
6337
6338 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6336.304 kb on - strand, within Pf6N2E2_5516 at 6336.447 kb on + strand at 6336.447 kb on + strand at 6336.448 kb on - strand at 6336.525 kb on + strand at 6336.526 kb on - strand at 6336.526 kb on - strand at 6336.526 kb on - strand at 6336.630 kb on + strand, within Pf6N2E2_5517 at 6336.631 kb on - strand, within Pf6N2E2_5517 at 6336.631 kb on - strand, within Pf6N2E2_5517 at 6336.631 kb on - strand, within Pf6N2E2_5517 at 6336.688 kb on - strand, within Pf6N2E2_5517 at 6336.688 kb on - strand, within Pf6N2E2_5517 at 6336.762 kb on + strand, within Pf6N2E2_5517 at 6337.002 kb on + strand, within Pf6N2E2_5517 at 6337.002 kb on + strand, within Pf6N2E2_5517 at 6337.003 kb on - strand, within Pf6N2E2_5517 at 6337.068 kb on + strand, within Pf6N2E2_5517 at 6337.068 kb on + strand, within Pf6N2E2_5517 at 6337.068 kb on + strand, within Pf6N2E2_5517 at 6337.068 kb on + strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.101 kb on + strand, within Pf6N2E2_5517 at 6337.101 kb on + strand, within Pf6N2E2_5517 at 6337.102 kb on - strand, within Pf6N2E2_5517 at 6337.116 kb on + strand at 6337.394 kb on + strand, within Pf6N2E2_5518 at 6337.508 kb on + strand, within Pf6N2E2_5518 at 6337.508 kb on + strand, within Pf6N2E2_5518 at 6337.509 kb on - strand, within Pf6N2E2_5518 at 6337.509 kb on - strand, within Pf6N2E2_5518 at 6337.509 kb on - strand, within Pf6N2E2_5518 at 6337.509 kb on - strand, within Pf6N2E2_5518 at 6337.509 kb on - strand, within Pf6N2E2_5518 at 6337.509 kb on - strand, within Pf6N2E2_5518 at 6337.509 kb on - strand, within Pf6N2E2_5518 at 6337.509 kb on - strand, within Pf6N2E2_5518 at 6337.565 kb on + strand, within Pf6N2E2_5518 at 6337.566 kb on - strand, within Pf6N2E2_5518 at 6337.653 kb on + strand, within Pf6N2E2_5518 at 6337.654 kb on - strand, within Pf6N2E2_5518 at 6337.654 kb on - strand, within Pf6N2E2_5518 at 6337.683 kb on - strand, within Pf6N2E2_5518 at 6337.870 kb on + strand, within Pf6N2E2_5518 at 6337.959 kb on + strand, within Pf6N2E2_5518 at 6337.959 kb on + strand, within Pf6N2E2_5518 at 6337.960 kb on - strand, within Pf6N2E2_5518 at 6337.960 kb on - strand, within Pf6N2E2_5518 at 6338.009 kb on - strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.019 kb on - strand, within Pf6N2E2_5518
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 6,336,304 - Pf6N2E2_5516 0.88 +1.7 6,336,447 + -0.8 6,336,447 + +0.4 6,336,448 - +0.5 6,336,525 + -2.6 6,336,526 - +0.6 6,336,526 - -2.3 6,336,526 - +0.0 6,336,630 + Pf6N2E2_5517 0.14 +0.3 6,336,631 - Pf6N2E2_5517 0.14 +0.2 6,336,631 - Pf6N2E2_5517 0.14 +1.4 6,336,631 - Pf6N2E2_5517 0.14 +0.1 6,336,688 - Pf6N2E2_5517 0.23 -0.2 6,336,688 - Pf6N2E2_5517 0.23 +0.5 6,336,762 + Pf6N2E2_5517 0.35 +0.5 6,337,002 + Pf6N2E2_5517 0.73 -0.7 6,337,002 + Pf6N2E2_5517 0.73 +0.1 6,337,003 - Pf6N2E2_5517 0.73 +2.0 6,337,068 + Pf6N2E2_5517 0.83 +0.2 6,337,068 + Pf6N2E2_5517 0.83 +0.6 6,337,068 + Pf6N2E2_5517 0.83 +0.5 6,337,068 + Pf6N2E2_5517 0.83 +1.2 6,337,069 - Pf6N2E2_5517 0.83 -0.0 6,337,069 - Pf6N2E2_5517 0.83 +0.2 6,337,069 - Pf6N2E2_5517 0.83 -0.9 6,337,069 - Pf6N2E2_5517 0.83 +0.9 6,337,069 - Pf6N2E2_5517 0.83 -0.4 6,337,101 + Pf6N2E2_5517 0.88 +0.1 6,337,101 + Pf6N2E2_5517 0.88 -0.9 6,337,102 - Pf6N2E2_5517 0.88 -0.4 6,337,116 + +2.4 6,337,394 + Pf6N2E2_5518 0.22 +0.2 6,337,508 + Pf6N2E2_5518 0.35 -0.2 6,337,508 + Pf6N2E2_5518 0.35 -0.4 6,337,509 - Pf6N2E2_5518 0.35 +0.8 6,337,509 - Pf6N2E2_5518 0.35 +0.3 6,337,509 - Pf6N2E2_5518 0.35 -2.9 6,337,509 - Pf6N2E2_5518 0.35 -1.2 6,337,509 - Pf6N2E2_5518 0.35 -1.1 6,337,509 - Pf6N2E2_5518 0.35 -1.6 6,337,509 - Pf6N2E2_5518 0.35 +1.5 6,337,509 - Pf6N2E2_5518 0.35 -0.7 6,337,565 + Pf6N2E2_5518 0.41 -1.6 6,337,566 - Pf6N2E2_5518 0.41 -0.6 6,337,653 + Pf6N2E2_5518 0.50 +0.0 6,337,654 - Pf6N2E2_5518 0.50 +0.3 6,337,654 - Pf6N2E2_5518 0.50 +1.4 6,337,683 - Pf6N2E2_5518 0.53 -0.3 6,337,870 + Pf6N2E2_5518 0.73 +0.6 6,337,959 + Pf6N2E2_5518 0.83 +0.6 6,337,959 + Pf6N2E2_5518 0.83 +0.2 6,337,960 - Pf6N2E2_5518 0.83 +0.7 6,337,960 - Pf6N2E2_5518 0.83 +1.2 6,338,009 - Pf6N2E2_5518 0.88 -0.7 6,338,018 + Pf6N2E2_5518 0.89 +0.2 6,338,018 + Pf6N2E2_5518 0.89 +0.1 6,338,018 + Pf6N2E2_5518 0.89 -0.1 6,338,018 + Pf6N2E2_5518 0.89 +0.3 6,338,018 + Pf6N2E2_5518 0.89 +0.4 6,338,019 - Pf6N2E2_5518 0.89 +0.5
Or see this region's nucleotide sequence