Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4894 and Pf6N2E2_4895 are separated by 81 nucleotides Pf6N2E2_4895 and Pf6N2E2_4896 are separated by 98 nucleotides
Pf6N2E2_4894 - large transcriptional regulator, at 5,645,441 to 5,648,740
_4894
Pf6N2E2_4895 - Isochorismatase (EC 3.3.2.1), at 5,648,822 to 5,649,376
_4895
Pf6N2E2_4896 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,649,475 to 5,650,470
_4896
Position (kb)
5648
5649
5650 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5647.907 kb on + strand, within Pf6N2E2_4894 at 5647.907 kb on + strand, within Pf6N2E2_4894 at 5647.907 kb on + strand, within Pf6N2E2_4894 at 5647.908 kb on - strand, within Pf6N2E2_4894 at 5647.911 kb on - strand, within Pf6N2E2_4894 at 5647.938 kb on - strand, within Pf6N2E2_4894 at 5647.938 kb on - strand, within Pf6N2E2_4894 at 5647.959 kb on + strand, within Pf6N2E2_4894 at 5647.959 kb on + strand, within Pf6N2E2_4894 at 5648.033 kb on + strand, within Pf6N2E2_4894 at 5648.033 kb on + strand, within Pf6N2E2_4894 at 5648.033 kb on + strand, within Pf6N2E2_4894 at 5648.033 kb on + strand, within Pf6N2E2_4894 at 5648.034 kb on - strand, within Pf6N2E2_4894 at 5648.351 kb on + strand, within Pf6N2E2_4894 at 5648.351 kb on + strand, within Pf6N2E2_4894 at 5648.351 kb on + strand, within Pf6N2E2_4894 at 5648.351 kb on + strand, within Pf6N2E2_4894 at 5648.352 kb on - strand, within Pf6N2E2_4894 at 5648.352 kb on - strand, within Pf6N2E2_4894 at 5648.352 kb on - strand, within Pf6N2E2_4894 at 5648.352 kb on - strand, within Pf6N2E2_4894 at 5648.519 kb on - strand at 5648.530 kb on + strand at 5648.530 kb on + strand at 5648.752 kb on + strand at 5648.753 kb on - strand at 5648.753 kb on - strand at 5648.823 kb on + strand at 5648.824 kb on - strand at 5648.983 kb on - strand, within Pf6N2E2_4895 at 5649.086 kb on + strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.158 kb on - strand, within Pf6N2E2_4895 at 5649.333 kb on + strand at 5649.333 kb on + strand at 5649.334 kb on - strand at 5649.334 kb on - strand at 5649.334 kb on - strand at 5649.334 kb on - strand at 5649.334 kb on - strand at 5649.354 kb on - strand at 5649.422 kb on + strand at 5649.455 kb on + strand at 5649.455 kb on + strand at 5649.455 kb on + strand at 5649.455 kb on + strand at 5649.456 kb on - strand at 5649.456 kb on - strand at 5649.456 kb on - strand at 5649.456 kb on - strand at 5649.517 kb on + strand at 5649.517 kb on + strand at 5649.517 kb on + strand at 5649.518 kb on - strand at 5650.001 kb on + strand, within Pf6N2E2_4896 at 5650.099 kb on - strand, within Pf6N2E2_4896 at 5650.224 kb on + strand, within Pf6N2E2_4896 at 5650.224 kb on + strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.273 kb on + strand, within Pf6N2E2_4896 at 5650.274 kb on - strand, within Pf6N2E2_4896 at 5650.274 kb on - strand, within Pf6N2E2_4896 at 5650.274 kb on - strand, within Pf6N2E2_4896
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 5,647,907 + Pf6N2E2_4894 0.75 -0.2 5,647,907 + Pf6N2E2_4894 0.75 -1.7 5,647,907 + Pf6N2E2_4894 0.75 -0.4 5,647,908 - Pf6N2E2_4894 0.75 +0.3 5,647,911 - Pf6N2E2_4894 0.75 +1.0 5,647,938 - Pf6N2E2_4894 0.76 -1.6 5,647,938 - Pf6N2E2_4894 0.76 +1.7 5,647,959 + Pf6N2E2_4894 0.76 +0.4 5,647,959 + Pf6N2E2_4894 0.76 -0.4 5,648,033 + Pf6N2E2_4894 0.79 -0.6 5,648,033 + Pf6N2E2_4894 0.79 -0.2 5,648,033 + Pf6N2E2_4894 0.79 +0.0 5,648,033 + Pf6N2E2_4894 0.79 -0.3 5,648,034 - Pf6N2E2_4894 0.79 +0.8 5,648,351 + Pf6N2E2_4894 0.88 +0.1 5,648,351 + Pf6N2E2_4894 0.88 -0.2 5,648,351 + Pf6N2E2_4894 0.88 -3.4 5,648,351 + Pf6N2E2_4894 0.88 +1.0 5,648,352 - Pf6N2E2_4894 0.88 +0.0 5,648,352 - Pf6N2E2_4894 0.88 -0.4 5,648,352 - Pf6N2E2_4894 0.88 +1.0 5,648,352 - Pf6N2E2_4894 0.88 +0.2 5,648,519 - -0.6 5,648,530 + +0.7 5,648,530 + +0.7 5,648,752 + +0.6 5,648,753 - -0.3 5,648,753 - +1.7 5,648,823 + -0.7 5,648,824 - +0.1 5,648,983 - Pf6N2E2_4895 0.29 +0.2 5,649,086 + Pf6N2E2_4895 0.48 +0.6 5,649,087 - Pf6N2E2_4895 0.48 +1.0 5,649,087 - Pf6N2E2_4895 0.48 +1.4 5,649,087 - Pf6N2E2_4895 0.48 +0.2 5,649,087 - Pf6N2E2_4895 0.48 +0.6 5,649,087 - Pf6N2E2_4895 0.48 -0.0 5,649,087 - Pf6N2E2_4895 0.48 +0.7 5,649,158 - Pf6N2E2_4895 0.61 -0.0 5,649,333 + +0.6 5,649,333 + +0.1 5,649,334 - +0.4 5,649,334 - +1.4 5,649,334 - -1.3 5,649,334 - +0.2 5,649,334 - -0.1 5,649,354 - +0.7 5,649,422 + +0.1 5,649,455 + +0.8 5,649,455 + -0.2 5,649,455 + -0.4 5,649,455 + -0.3 5,649,456 - +0.7 5,649,456 - +0.1 5,649,456 - +1.8 5,649,456 - -0.1 5,649,517 + +0.4 5,649,517 + -0.2 5,649,517 + -0.3 5,649,518 - +1.0 5,650,001 + Pf6N2E2_4896 0.53 -1.2 5,650,099 - Pf6N2E2_4896 0.63 +0.2 5,650,224 + Pf6N2E2_4896 0.75 +0.3 5,650,224 + Pf6N2E2_4896 0.75 +0.6 5,650,225 - Pf6N2E2_4896 0.75 +0.2 5,650,225 - Pf6N2E2_4896 0.75 -0.9 5,650,225 - Pf6N2E2_4896 0.75 +0.1 5,650,225 - Pf6N2E2_4896 0.75 +0.6 5,650,273 + Pf6N2E2_4896 0.80 +0.8 5,650,274 - Pf6N2E2_4896 0.80 +1.4 5,650,274 - Pf6N2E2_4896 0.80 +1.1 5,650,274 - Pf6N2E2_4896 0.80 -0.4
Or see this region's nucleotide sequence