Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3171 and Pf6N2E2_3172 are separated by 63 nucleotides Pf6N2E2_3172 and Pf6N2E2_3173 overlap by 13 nucleotides Pf6N2E2_3173 and Pf6N2E2_3174 are separated by 270 nucleotides
Pf6N2E2_3171 - hypothetical protein, at 3,667,027 to 3,667,440
_3171
Pf6N2E2_3172 - Aliphatic amidase AmiE (EC 3.5.1.4), at 3,667,504 to 3,668,298
_3172
Pf6N2E2_3173 - Aspartate aminotransferase (EC 2.6.1.1), at 3,668,286 to 3,669,434
_3173
Pf6N2E2_3174 - GTP-binding protein EngA, at 3,669,705 to 3,671,177
_3174
Position (kb)
3668
3669
3670 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3667.389 kb on + strand, within Pf6N2E2_3171 at 3667.414 kb on + strand at 3667.414 kb on + strand at 3667.431 kb on + strand at 3667.432 kb on - strand at 3667.440 kb on + strand at 3667.559 kb on + strand at 3667.667 kb on + strand, within Pf6N2E2_3172 at 3667.667 kb on + strand, within Pf6N2E2_3172 at 3667.668 kb on - strand, within Pf6N2E2_3172 at 3667.715 kb on + strand, within Pf6N2E2_3172 at 3667.716 kb on - strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.795 kb on + strand, within Pf6N2E2_3172 at 3667.795 kb on + strand, within Pf6N2E2_3172 at 3667.796 kb on - strand, within Pf6N2E2_3172 at 3667.796 kb on - strand, within Pf6N2E2_3172 at 3667.851 kb on - strand, within Pf6N2E2_3172 at 3667.916 kb on + strand, within Pf6N2E2_3172 at 3667.917 kb on - strand, within Pf6N2E2_3172 at 3667.961 kb on + strand, within Pf6N2E2_3172 at 3667.962 kb on - strand, within Pf6N2E2_3172 at 3667.962 kb on - strand, within Pf6N2E2_3172 at 3667.962 kb on - strand, within Pf6N2E2_3172 at 3668.298 kb on + strand at 3668.298 kb on + strand at 3668.299 kb on - strand at 3668.299 kb on - strand at 3668.299 kb on - strand at 3668.383 kb on + strand at 3668.384 kb on - strand at 3668.384 kb on - strand at 3668.476 kb on + strand, within Pf6N2E2_3173 at 3668.494 kb on + strand, within Pf6N2E2_3173 at 3668.494 kb on + strand, within Pf6N2E2_3173 at 3668.495 kb on - strand, within Pf6N2E2_3173 at 3668.495 kb on - strand, within Pf6N2E2_3173 at 3668.495 kb on - strand, within Pf6N2E2_3173 at 3668.569 kb on + strand, within Pf6N2E2_3173 at 3668.570 kb on - strand, within Pf6N2E2_3173 at 3668.570 kb on - strand, within Pf6N2E2_3173 at 3668.614 kb on + strand, within Pf6N2E2_3173 at 3668.614 kb on + strand, within Pf6N2E2_3173 at 3668.614 kb on + strand, within Pf6N2E2_3173 at 3668.615 kb on - strand, within Pf6N2E2_3173 at 3668.615 kb on - strand, within Pf6N2E2_3173 at 3668.629 kb on + strand, within Pf6N2E2_3173 at 3668.629 kb on + strand, within Pf6N2E2_3173 at 3668.662 kb on + strand, within Pf6N2E2_3173 at 3668.662 kb on + strand, within Pf6N2E2_3173 at 3668.662 kb on + strand, within Pf6N2E2_3173 at 3668.662 kb on + strand, within Pf6N2E2_3173 at 3668.663 kb on - strand, within Pf6N2E2_3173 at 3668.663 kb on - strand, within Pf6N2E2_3173 at 3668.663 kb on - strand, within Pf6N2E2_3173 at 3668.663 kb on - strand, within Pf6N2E2_3173 at 3668.670 kb on + strand, within Pf6N2E2_3173 at 3668.670 kb on + strand, within Pf6N2E2_3173 at 3668.670 kb on + strand, within Pf6N2E2_3173 at 3668.670 kb on + strand, within Pf6N2E2_3173 at 3668.670 kb on + strand, within Pf6N2E2_3173 at 3668.671 kb on - strand, within Pf6N2E2_3173 at 3668.740 kb on + strand, within Pf6N2E2_3173 at 3668.741 kb on - strand, within Pf6N2E2_3173 at 3668.741 kb on - strand, within Pf6N2E2_3173 at 3668.741 kb on - strand, within Pf6N2E2_3173 at 3668.839 kb on + strand, within Pf6N2E2_3173 at 3668.860 kb on + strand, within Pf6N2E2_3173 at 3668.860 kb on + strand, within Pf6N2E2_3173 at 3668.860 kb on + strand, within Pf6N2E2_3173 at 3668.860 kb on + strand, within Pf6N2E2_3173 at 3668.861 kb on - strand, within Pf6N2E2_3173 at 3668.861 kb on - strand, within Pf6N2E2_3173 at 3668.861 kb on - strand, within Pf6N2E2_3173 at 3668.861 kb on - strand, within Pf6N2E2_3173 at 3668.861 kb on - strand, within Pf6N2E2_3173 at 3668.861 kb on - strand at 3669.070 kb on + strand, within Pf6N2E2_3173 at 3669.070 kb on + strand, within Pf6N2E2_3173 at 3669.070 kb on + strand, within Pf6N2E2_3173 at 3669.070 kb on + strand, within Pf6N2E2_3173 at 3669.070 kb on + strand, within Pf6N2E2_3173 at 3669.071 kb on - strand, within Pf6N2E2_3173 at 3669.071 kb on - strand, within Pf6N2E2_3173 at 3669.071 kb on - strand, within Pf6N2E2_3173 at 3669.071 kb on - strand, within Pf6N2E2_3173 at 3669.079 kb on + strand, within Pf6N2E2_3173 at 3669.079 kb on + strand, within Pf6N2E2_3173 at 3669.079 kb on + strand, within Pf6N2E2_3173 at 3669.080 kb on - strand, within Pf6N2E2_3173 at 3669.080 kb on - strand, within Pf6N2E2_3173 at 3669.080 kb on - strand, within Pf6N2E2_3173 at 3669.083 kb on + strand, within Pf6N2E2_3173 at 3669.083 kb on + strand, within Pf6N2E2_3173 at 3669.083 kb on + strand, within Pf6N2E2_3173 at 3669.084 kb on - strand, within Pf6N2E2_3173 at 3669.084 kb on - strand, within Pf6N2E2_3173 at 3669.203 kb on - strand, within Pf6N2E2_3173 at 3669.266 kb on - strand, within Pf6N2E2_3173 at 3669.398 kb on + strand at 3669.398 kb on + strand at 3669.398 kb on + strand at 3669.398 kb on + strand at 3669.398 kb on + strand at 3669.398 kb on + strand at 3669.489 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 3,667,389 + Pf6N2E2_3171 0.87 +0.5 3,667,414 + -0.2 3,667,414 + -0.7 3,667,431 + +1.7 3,667,432 - +0.4 3,667,440 + +0.5 3,667,559 + -0.4 3,667,667 + Pf6N2E2_3172 0.21 +0.1 3,667,667 + Pf6N2E2_3172 0.21 +0.3 3,667,668 - Pf6N2E2_3172 0.21 -0.5 3,667,715 + Pf6N2E2_3172 0.27 +0.1 3,667,716 - Pf6N2E2_3172 0.27 +0.4 3,667,793 + Pf6N2E2_3172 0.36 +0.2 3,667,793 + Pf6N2E2_3172 0.36 -1.0 3,667,793 + Pf6N2E2_3172 0.36 -0.4 3,667,793 + Pf6N2E2_3172 0.36 +0.7 3,667,793 + Pf6N2E2_3172 0.36 -0.8 3,667,794 - Pf6N2E2_3172 0.36 -0.5 3,667,794 - Pf6N2E2_3172 0.36 +0.0 3,667,794 - Pf6N2E2_3172 0.36 +0.3 3,667,794 - Pf6N2E2_3172 0.36 +0.5 3,667,794 - Pf6N2E2_3172 0.36 -0.5 3,667,794 - Pf6N2E2_3172 0.36 +0.6 3,667,795 + Pf6N2E2_3172 0.37 +1.1 3,667,795 + Pf6N2E2_3172 0.37 -0.2 3,667,796 - Pf6N2E2_3172 0.37 +0.4 3,667,796 - Pf6N2E2_3172 0.37 -0.5 3,667,851 - Pf6N2E2_3172 0.44 -0.3 3,667,916 + Pf6N2E2_3172 0.52 +0.2 3,667,917 - Pf6N2E2_3172 0.52 -0.6 3,667,961 + Pf6N2E2_3172 0.57 +0.7 3,667,962 - Pf6N2E2_3172 0.58 +0.1 3,667,962 - Pf6N2E2_3172 0.58 -0.4 3,667,962 - Pf6N2E2_3172 0.58 -1.2 3,668,298 + +0.3 3,668,298 + -0.9 3,668,299 - +0.0 3,668,299 - +0.4 3,668,299 - +0.4 3,668,383 + +0.2 3,668,384 - +0.1 3,668,384 - +0.3 3,668,476 + Pf6N2E2_3173 0.17 +2.0 3,668,494 + Pf6N2E2_3173 0.18 -0.3 3,668,494 + Pf6N2E2_3173 0.18 -1.2 3,668,495 - Pf6N2E2_3173 0.18 +0.1 3,668,495 - Pf6N2E2_3173 0.18 +0.4 3,668,495 - Pf6N2E2_3173 0.18 -0.4 3,668,569 + Pf6N2E2_3173 0.25 +1.5 3,668,570 - Pf6N2E2_3173 0.25 -0.4 3,668,570 - Pf6N2E2_3173 0.25 -1.2 3,668,614 + Pf6N2E2_3173 0.29 +0.1 3,668,614 + Pf6N2E2_3173 0.29 +1.2 3,668,614 + Pf6N2E2_3173 0.29 -0.0 3,668,615 - Pf6N2E2_3173 0.29 +0.5 3,668,615 - Pf6N2E2_3173 0.29 +0.1 3,668,629 + Pf6N2E2_3173 0.30 +1.1 3,668,629 + Pf6N2E2_3173 0.30 +0.2 3,668,662 + Pf6N2E2_3173 0.33 +0.4 3,668,662 + Pf6N2E2_3173 0.33 -0.0 3,668,662 + Pf6N2E2_3173 0.33 +0.8 3,668,662 + Pf6N2E2_3173 0.33 +0.9 3,668,663 - Pf6N2E2_3173 0.33 -0.1 3,668,663 - Pf6N2E2_3173 0.33 -0.2 3,668,663 - Pf6N2E2_3173 0.33 -0.0 3,668,663 - Pf6N2E2_3173 0.33 +0.4 3,668,670 + Pf6N2E2_3173 0.33 +0.8 3,668,670 + Pf6N2E2_3173 0.33 +0.7 3,668,670 + Pf6N2E2_3173 0.33 -0.6 3,668,670 + Pf6N2E2_3173 0.33 -0.2 3,668,670 + Pf6N2E2_3173 0.33 -0.3 3,668,671 - Pf6N2E2_3173 0.34 +0.5 3,668,740 + Pf6N2E2_3173 0.40 +0.6 3,668,741 - Pf6N2E2_3173 0.40 -0.4 3,668,741 - Pf6N2E2_3173 0.40 +0.8 3,668,741 - Pf6N2E2_3173 0.40 +0.5 3,668,839 + Pf6N2E2_3173 0.48 -1.2 3,668,860 + Pf6N2E2_3173 0.50 +0.3 3,668,860 + Pf6N2E2_3173 0.50 -1.0 3,668,860 + Pf6N2E2_3173 0.50 +0.8 3,668,860 + Pf6N2E2_3173 0.50 +1.1 3,668,861 - Pf6N2E2_3173 0.50 +1.1 3,668,861 - Pf6N2E2_3173 0.50 +0.6 3,668,861 - Pf6N2E2_3173 0.50 +0.1 3,668,861 - Pf6N2E2_3173 0.50 -0.7 3,668,861 - Pf6N2E2_3173 0.50 +0.3 3,668,861 - +1.7 3,669,070 + Pf6N2E2_3173 0.68 -0.5 3,669,070 + Pf6N2E2_3173 0.68 +0.4 3,669,070 + Pf6N2E2_3173 0.68 +0.5 3,669,070 + Pf6N2E2_3173 0.68 -0.5 3,669,070 + Pf6N2E2_3173 0.68 -1.2 3,669,071 - Pf6N2E2_3173 0.68 -1.0 3,669,071 - Pf6N2E2_3173 0.68 -0.2 3,669,071 - Pf6N2E2_3173 0.68 -1.1 3,669,071 - Pf6N2E2_3173 0.68 -0.2 3,669,079 + Pf6N2E2_3173 0.69 +0.4 3,669,079 + Pf6N2E2_3173 0.69 -2.2 3,669,079 + Pf6N2E2_3173 0.69 -1.2 3,669,080 - Pf6N2E2_3173 0.69 +0.1 3,669,080 - Pf6N2E2_3173 0.69 +1.0 3,669,080 - Pf6N2E2_3173 0.69 -1.7 3,669,083 + Pf6N2E2_3173 0.69 -0.9 3,669,083 + Pf6N2E2_3173 0.69 -0.3 3,669,083 + Pf6N2E2_3173 0.69 +1.3 3,669,084 - Pf6N2E2_3173 0.69 -1.3 3,669,084 - Pf6N2E2_3173 0.69 -0.1 3,669,203 - Pf6N2E2_3173 0.80 +1.2 3,669,266 - Pf6N2E2_3173 0.85 +1.3 3,669,398 + +0.6 3,669,398 + -1.2 3,669,398 + -0.5 3,669,398 + +0.1 3,669,398 + -0.1 3,669,398 + -0.5 3,669,489 + -1.2
Or see this region's nucleotide sequence