Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_302 and Pf6N2E2_303 are separated by 127 nucleotides Pf6N2E2_303 and Pf6N2E2_304 are separated by 88 nucleotides
Pf6N2E2_302 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 372,395 to 373,789
_302
Pf6N2E2_303 - Glutathione S-transferase, omega (EC 2.5.1.18), at 373,917 to 374,915
_303
Pf6N2E2_304 - Anthranilate phosphoribosyltransferase like (EC 2.4.2.18), at 375,004 to 376,005
_304
Position (kb) 373
374
375 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 373.049 kb on + strand, within Pf6N2E2_302 at 373.193 kb on + strand, within Pf6N2E2_302 at 373.193 kb on + strand, within Pf6N2E2_302 at 373.440 kb on - strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.538 kb on + strand, within Pf6N2E2_302 at 373.539 kb on - strand, within Pf6N2E2_302 at 373.539 kb on - strand, within Pf6N2E2_302 at 373.539 kb on - strand, within Pf6N2E2_302 at 373.539 kb on - strand, within Pf6N2E2_302 at 373.755 kb on + strand at 373.755 kb on + strand at 373.787 kb on + strand at 373.788 kb on - strand at 374.035 kb on + strand, within Pf6N2E2_303 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.052 kb on - strand, within Pf6N2E2_303 at 374.092 kb on + strand, within Pf6N2E2_303 at 374.167 kb on + strand, within Pf6N2E2_303 at 374.167 kb on + strand, within Pf6N2E2_303 at 374.168 kb on - strand, within Pf6N2E2_303 at 374.225 kb on - strand, within Pf6N2E2_303 at 374.225 kb on - strand, within Pf6N2E2_303 at 374.239 kb on + strand, within Pf6N2E2_303 at 374.308 kb on + strand, within Pf6N2E2_303 at 374.309 kb on - strand, within Pf6N2E2_303 at 374.366 kb on - strand, within Pf6N2E2_303 at 374.545 kb on + strand, within Pf6N2E2_303 at 374.546 kb on - strand, within Pf6N2E2_303 at 374.566 kb on + strand, within Pf6N2E2_303 at 374.656 kb on + strand, within Pf6N2E2_303 at 374.670 kb on + strand, within Pf6N2E2_303 at 374.670 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.875 kb on + strand at 374.875 kb on + strand at 374.875 kb on + strand at 374.876 kb on - strand at 374.876 kb on - strand at 374.904 kb on + strand at 374.953 kb on + strand at 374.973 kb on - strand at 375.005 kb on + strand at 375.032 kb on + strand at 375.123 kb on - strand, within Pf6N2E2_304 at 375.563 kb on - strand, within Pf6N2E2_304 at 375.599 kb on + strand, within Pf6N2E2_304 at 375.684 kb on - strand, within Pf6N2E2_304 at 375.713 kb on + strand, within Pf6N2E2_304 at 375.713 kb on + strand, within Pf6N2E2_304 at 375.820 kb on + strand, within Pf6N2E2_304
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 373,049 + Pf6N2E2_302 0.47 -2.4 373,193 + Pf6N2E2_302 0.57 -3.0 373,193 + Pf6N2E2_302 0.57 -3.9 373,440 - Pf6N2E2_302 0.75 -0.7 373,538 + Pf6N2E2_302 0.82 -2.5 373,538 + Pf6N2E2_302 0.82 -2.3 373,538 + Pf6N2E2_302 0.82 -3.7 373,538 + Pf6N2E2_302 0.82 -3.1 373,538 + Pf6N2E2_302 0.82 -1.7 373,539 - Pf6N2E2_302 0.82 -3.3 373,539 - Pf6N2E2_302 0.82 -2.1 373,539 - Pf6N2E2_302 0.82 -0.4 373,539 - Pf6N2E2_302 0.82 -3.5 373,755 + -2.3 373,755 + -0.6 373,787 + +0.9 373,788 - -2.8 374,035 + Pf6N2E2_303 0.12 -0.7 374,035 + Pf6N2E2_303 0.12 -0.3 374,035 + Pf6N2E2_303 0.12 -0.1 374,036 - Pf6N2E2_303 0.12 -0.2 374,036 - Pf6N2E2_303 0.12 -0.6 374,036 - Pf6N2E2_303 0.12 +0.2 374,036 - Pf6N2E2_303 0.12 -0.7 374,039 - Pf6N2E2_303 0.12 -0.1 374,039 - Pf6N2E2_303 0.12 -0.4 374,039 - Pf6N2E2_303 0.12 +0.6 374,052 - Pf6N2E2_303 0.14 +1.2 374,092 + Pf6N2E2_303 0.18 +0.0 374,167 + Pf6N2E2_303 0.25 +0.1 374,167 + Pf6N2E2_303 0.25 +0.2 374,168 - Pf6N2E2_303 0.25 +0.2 374,225 - Pf6N2E2_303 0.31 -0.2 374,225 - Pf6N2E2_303 0.31 -0.3 374,239 + Pf6N2E2_303 0.32 +0.7 374,308 + Pf6N2E2_303 0.39 +0.6 374,309 - Pf6N2E2_303 0.39 +0.5 374,366 - Pf6N2E2_303 0.45 +0.1 374,545 + Pf6N2E2_303 0.63 +0.4 374,546 - Pf6N2E2_303 0.63 +1.1 374,566 + Pf6N2E2_303 0.65 -0.0 374,656 + Pf6N2E2_303 0.74 -0.5 374,670 + Pf6N2E2_303 0.75 +0.7 374,670 + Pf6N2E2_303 0.75 +1.1 374,749 + Pf6N2E2_303 0.83 +0.5 374,749 + Pf6N2E2_303 0.83 +0.1 374,749 + Pf6N2E2_303 0.83 -0.3 374,749 + Pf6N2E2_303 0.83 +0.1 374,749 + Pf6N2E2_303 0.83 -0.2 374,749 + Pf6N2E2_303 0.83 -1.0 374,749 + Pf6N2E2_303 0.83 -1.5 374,749 + Pf6N2E2_303 0.83 +0.5 374,749 + Pf6N2E2_303 0.83 -1.1 374,749 + Pf6N2E2_303 0.83 +1.1 374,750 - Pf6N2E2_303 0.83 +1.2 374,750 - Pf6N2E2_303 0.83 -1.0 374,750 - Pf6N2E2_303 0.83 -0.4 374,750 - Pf6N2E2_303 0.83 -0.3 374,750 - Pf6N2E2_303 0.83 -1.1 374,750 - Pf6N2E2_303 0.83 -1.1 374,875 + +0.1 374,875 + +0.4 374,875 + +0.1 374,876 - +0.6 374,876 - -0.6 374,904 + +0.7 374,953 + -0.5 374,973 - +0.5 375,005 + -0.2 375,032 + +0.2 375,123 - Pf6N2E2_304 0.12 +0.8 375,563 - Pf6N2E2_304 0.56 -0.2 375,599 + Pf6N2E2_304 0.59 -1.6 375,684 - Pf6N2E2_304 0.68 -0.6 375,713 + Pf6N2E2_304 0.71 -0.1 375,713 + Pf6N2E2_304 0.71 +0.9 375,820 + Pf6N2E2_304 0.81 -1.7
Or see this region's nucleotide sequence