Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2630 and Pf6N2E2_2631 are separated by 54 nucleotides Pf6N2E2_2631 and Pf6N2E2_2632 overlap by 1 nucleotides Pf6N2E2_2632 and Pf6N2E2_2633 are separated by 192 nucleotides Pf6N2E2_2633 and Pf6N2E2_2634 are separated by 162 nucleotides
Pf6N2E2_2630 - Phenylalanine 4-monooxygenase (EC 1.14.16.1) (from data) , at 3,092,736 to 3,093,521
_2630
Pf6N2E2_2631 - Pterin-4-alpha-carbinolamine dehydratase (EC 4.2.1.96), at 3,093,576 to 3,093,977
_2631
Pf6N2E2_2632 - tyrosine aminotransferase (EC 2.6.1.57) (from data) , at 3,093,977 to 3,095,170
_2632
Pf6N2E2_2633 - Nitroreductase family protein, at 3,095,363 to 3,095,926
_2633
Pf6N2E2_2634 - Potassium uptake protein TrkH, at 3,096,089 to 3,097,543
_2634
Position (kb)
3093
3094
3095
3096 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3093.073 kb on - strand, within Pf6N2E2_2630 at 3093.073 kb on - strand, within Pf6N2E2_2630 at 3093.201 kb on + strand, within Pf6N2E2_2630 at 3093.216 kb on + strand, within Pf6N2E2_2630 at 3093.216 kb on + strand, within Pf6N2E2_2630 at 3093.270 kb on + strand, within Pf6N2E2_2630 at 3093.270 kb on + strand, within Pf6N2E2_2630 at 3093.309 kb on + strand, within Pf6N2E2_2630 at 3093.309 kb on + strand, within Pf6N2E2_2630 at 3093.310 kb on - strand, within Pf6N2E2_2630 at 3093.310 kb on - strand, within Pf6N2E2_2630 at 3093.400 kb on - strand, within Pf6N2E2_2630 at 3093.534 kb on - strand at 3093.534 kb on - strand at 3093.534 kb on - strand at 3093.600 kb on + strand at 3093.600 kb on + strand at 3093.601 kb on - strand at 3093.601 kb on - strand at 3093.975 kb on + strand at 3093.975 kb on + strand at 3093.975 kb on + strand at 3093.976 kb on - strand at 3093.976 kb on - strand at 3093.976 kb on - strand at 3093.976 kb on - strand at 3093.976 kb on - strand at 3093.976 kb on - strand at 3094.172 kb on + strand, within Pf6N2E2_2632 at 3094.172 kb on + strand, within Pf6N2E2_2632 at 3094.172 kb on + strand, within Pf6N2E2_2632 at 3094.224 kb on + strand, within Pf6N2E2_2632 at 3094.225 kb on - strand, within Pf6N2E2_2632 at 3094.225 kb on - strand, within Pf6N2E2_2632 at 3094.419 kb on - strand, within Pf6N2E2_2632 at 3094.424 kb on + strand, within Pf6N2E2_2632 at 3094.424 kb on + strand, within Pf6N2E2_2632 at 3094.425 kb on - strand, within Pf6N2E2_2632 at 3094.425 kb on - strand, within Pf6N2E2_2632 at 3094.727 kb on + strand, within Pf6N2E2_2632 at 3095.025 kb on - strand, within Pf6N2E2_2632 at 3095.078 kb on + strand at 3095.078 kb on + strand at 3095.078 kb on + strand at 3095.079 kb on - strand at 3095.079 kb on - strand at 3095.079 kb on - strand at 3095.079 kb on - strand at 3095.079 kb on - strand at 3095.509 kb on - strand, within Pf6N2E2_2633 at 3095.553 kb on + strand, within Pf6N2E2_2633 at 3095.554 kb on - strand, within Pf6N2E2_2633 at 3095.554 kb on - strand, within Pf6N2E2_2633 at 3095.954 kb on + strand at 3095.954 kb on + strand at 3095.954 kb on + strand at 3095.955 kb on - strand at 3095.955 kb on - strand at 3095.955 kb on - strand at 3095.955 kb on - strand at 3095.959 kb on + strand at 3095.959 kb on + strand at 3095.959 kb on + strand at 3095.960 kb on - strand at 3095.960 kb on - strand at 3096.050 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 3,093,073 - Pf6N2E2_2630 0.43 -2.5 3,093,073 - Pf6N2E2_2630 0.43 -2.7 3,093,201 + Pf6N2E2_2630 0.59 -3.4 3,093,216 + Pf6N2E2_2630 0.61 -3.6 3,093,216 + Pf6N2E2_2630 0.61 -4.2 3,093,270 + Pf6N2E2_2630 0.68 -3.4 3,093,270 + Pf6N2E2_2630 0.68 -4.1 3,093,309 + Pf6N2E2_2630 0.73 -2.3 3,093,309 + Pf6N2E2_2630 0.73 -3.9 3,093,310 - Pf6N2E2_2630 0.73 -0.5 3,093,310 - Pf6N2E2_2630 0.73 -2.4 3,093,400 - Pf6N2E2_2630 0.84 -5.0 3,093,534 - -2.9 3,093,534 - -3.5 3,093,534 - -2.9 3,093,600 + -0.5 3,093,600 + -0.7 3,093,601 - -3.6 3,093,601 - -1.8 3,093,975 + -2.7 3,093,975 + -4.9 3,093,975 + -3.1 3,093,976 - -3.5 3,093,976 - -2.9 3,093,976 - -2.7 3,093,976 - -4.3 3,093,976 - -4.6 3,093,976 - -1.5 3,094,172 + Pf6N2E2_2632 0.16 -1.9 3,094,172 + Pf6N2E2_2632 0.16 -3.9 3,094,172 + Pf6N2E2_2632 0.16 -1.0 3,094,224 + Pf6N2E2_2632 0.21 -3.5 3,094,225 - Pf6N2E2_2632 0.21 -3.3 3,094,225 - Pf6N2E2_2632 0.21 -2.5 3,094,419 - Pf6N2E2_2632 0.37 -4.2 3,094,424 + Pf6N2E2_2632 0.37 -4.6 3,094,424 + Pf6N2E2_2632 0.37 -3.0 3,094,425 - Pf6N2E2_2632 0.38 -4.1 3,094,425 - Pf6N2E2_2632 0.38 -3.1 3,094,727 + Pf6N2E2_2632 0.63 -4.0 3,095,025 - Pf6N2E2_2632 0.88 -3.7 3,095,078 + -4.1 3,095,078 + -4.8 3,095,078 + -4.1 3,095,079 - -2.4 3,095,079 - -3.2 3,095,079 - -3.7 3,095,079 - -2.9 3,095,079 - -4.7 3,095,509 - Pf6N2E2_2633 0.26 -0.0 3,095,553 + Pf6N2E2_2633 0.34 +0.5 3,095,554 - Pf6N2E2_2633 0.34 -1.9 3,095,554 - Pf6N2E2_2633 0.34 -0.5 3,095,954 + -0.1 3,095,954 + -1.1 3,095,954 + +0.4 3,095,955 - +0.3 3,095,955 - +0.1 3,095,955 - +0.3 3,095,955 - -0.4 3,095,959 + -0.3 3,095,959 + -2.2 3,095,959 + -0.3 3,095,960 - -2.1 3,095,960 - -0.4 3,096,050 + +1.8
Or see this region's nucleotide sequence