Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2550 and Pf6N2E2_2551 are separated by 389 nucleotides Pf6N2E2_2551 and Pf6N2E2_2552 are separated by 14 nucleotides Pf6N2E2_2552 and Pf6N2E2_2553 overlap by 8 nucleotides Pf6N2E2_2553 and Pf6N2E2_2554 overlap by 4 nucleotides Pf6N2E2_2554 and Pf6N2E2_2555 overlap by 4 nucleotides
Pf6N2E2_2550 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 3,009,761 to 3,011,854
_2550
Pf6N2E2_2551 - Cytochrome c heme lyase subunit CcmH, at 3,012,244 to 3,012,651
_2551
Pf6N2E2_2552 - Cytochrome c heme lyase subunit CcmH, at 3,012,666 to 3,013,868
_2552
Pf6N2E2_2553 - Cytochrome c heme lyase subunit CcmL, at 3,013,861 to 3,014,334
_2553
Pf6N2E2_2554 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 3,014,331 to 3,014,867
_2554
Pf6N2E2_2555 - Cytochrome c heme lyase subunit CcmF, at 3,014,864 to 3,016,852
_2555
Position (kb)
3012
3013
3014 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3011.708 kb on - strand at 3011.709 kb on + strand at 3011.709 kb on + strand at 3011.710 kb on - strand at 3011.710 kb on - strand at 3011.710 kb on - strand at 3011.710 kb on - strand at 3011.776 kb on - strand at 3011.776 kb on - strand at 3011.818 kb on + strand at 3011.819 kb on - strand at 3011.819 kb on - strand at 3011.819 kb on - strand at 3011.819 kb on - strand at 3011.821 kb on + strand at 3011.822 kb on - strand at 3011.822 kb on - strand at 3011.984 kb on + strand at 3011.984 kb on + strand at 3011.984 kb on + strand at 3011.984 kb on + strand at 3011.984 kb on + strand at 3012.031 kb on - strand at 3012.031 kb on - strand at 3012.054 kb on - strand at 3012.122 kb on - strand at 3012.122 kb on - strand at 3012.176 kb on + strand at 3012.176 kb on + strand at 3012.177 kb on - strand at 3012.246 kb on - strand at 3012.246 kb on - strand at 3012.246 kb on - strand at 3012.347 kb on + strand, within Pf6N2E2_2551 at 3012.350 kb on + strand, within Pf6N2E2_2551 at 3012.350 kb on + strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.396 kb on - strand, within Pf6N2E2_2551 at 3012.396 kb on - strand, within Pf6N2E2_2551 at 3012.396 kb on - strand, within Pf6N2E2_2551 at 3012.396 kb on - strand, within Pf6N2E2_2551 at 3012.459 kb on + strand, within Pf6N2E2_2551 at 3012.459 kb on + strand, within Pf6N2E2_2551 at 3012.459 kb on + strand, within Pf6N2E2_2551 at 3012.633 kb on + strand at 3012.634 kb on - strand at 3012.634 kb on - strand at 3012.634 kb on - strand at 3012.668 kb on - strand at 3013.160 kb on - strand, within Pf6N2E2_2552 at 3013.257 kb on - strand, within Pf6N2E2_2552 at 3013.390 kb on + strand, within Pf6N2E2_2552 at 3013.390 kb on + strand, within Pf6N2E2_2552 at 3013.390 kb on + strand, within Pf6N2E2_2552 at 3013.390 kb on + strand at 3013.390 kb on + strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.394 kb on + strand, within Pf6N2E2_2552 at 3013.408 kb on + strand, within Pf6N2E2_2552 at 3013.408 kb on + strand, within Pf6N2E2_2552 at 3013.409 kb on - strand, within Pf6N2E2_2552 at 3013.541 kb on - strand, within Pf6N2E2_2552 at 3013.541 kb on - strand, within Pf6N2E2_2552 at 3013.741 kb on + strand, within Pf6N2E2_2552 at 3013.742 kb on - strand, within Pf6N2E2_2552 at 3014.363 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 3,011,708 - -0.5 3,011,709 + +0.1 3,011,709 + -0.4 3,011,710 - -1.2 3,011,710 - +0.9 3,011,710 - -1.6 3,011,710 - -0.1 3,011,776 - -0.2 3,011,776 - +0.2 3,011,818 + +1.2 3,011,819 - -0.7 3,011,819 - +0.8 3,011,819 - -0.2 3,011,819 - -1.8 3,011,821 + -0.1 3,011,822 - -0.6 3,011,822 - +0.1 3,011,984 + -0.7 3,011,984 + +0.0 3,011,984 + +0.3 3,011,984 + -0.3 3,011,984 + +0.4 3,012,031 - -1.2 3,012,031 - +0.2 3,012,054 - -1.8 3,012,122 - +0.4 3,012,122 - -0.8 3,012,176 + +0.7 3,012,176 + -0.3 3,012,177 - -0.8 3,012,246 - -0.6 3,012,246 - +0.1 3,012,246 - -0.2 3,012,347 + Pf6N2E2_2551 0.25 -0.1 3,012,350 + Pf6N2E2_2551 0.26 +0.6 3,012,350 + Pf6N2E2_2551 0.26 +0.0 3,012,351 - Pf6N2E2_2551 0.26 +1.5 3,012,351 - Pf6N2E2_2551 0.26 +1.3 3,012,351 - Pf6N2E2_2551 0.26 +0.2 3,012,351 - Pf6N2E2_2551 0.26 -0.0 3,012,351 - Pf6N2E2_2551 0.26 -0.8 3,012,395 + Pf6N2E2_2551 0.37 +1.4 3,012,395 + Pf6N2E2_2551 0.37 -0.5 3,012,395 + Pf6N2E2_2551 0.37 -0.3 3,012,395 + Pf6N2E2_2551 0.37 +0.7 3,012,395 + Pf6N2E2_2551 0.37 -0.1 3,012,396 - Pf6N2E2_2551 0.37 +0.4 3,012,396 - Pf6N2E2_2551 0.37 +1.6 3,012,396 - Pf6N2E2_2551 0.37 -0.2 3,012,396 - Pf6N2E2_2551 0.37 +0.3 3,012,459 + Pf6N2E2_2551 0.53 +1.3 3,012,459 + Pf6N2E2_2551 0.53 +0.4 3,012,459 + Pf6N2E2_2551 0.53 -0.1 3,012,633 + +1.3 3,012,634 - -0.4 3,012,634 - +0.7 3,012,634 - -0.1 3,012,668 - -0.3 3,013,160 - Pf6N2E2_2552 0.41 +0.2 3,013,257 - Pf6N2E2_2552 0.49 -1.2 3,013,390 + Pf6N2E2_2552 0.60 -3.8 3,013,390 + Pf6N2E2_2552 0.60 -3.7 3,013,390 + Pf6N2E2_2552 0.60 -1.0 3,013,390 + +1.4 3,013,390 + Pf6N2E2_2552 0.60 -1.0 3,013,391 - Pf6N2E2_2552 0.60 -0.2 3,013,391 - Pf6N2E2_2552 0.60 -2.7 3,013,391 - Pf6N2E2_2552 0.60 -1.0 3,013,391 - Pf6N2E2_2552 0.60 -2.1 3,013,391 - Pf6N2E2_2552 0.60 -1.8 3,013,394 + Pf6N2E2_2552 0.61 -3.7 3,013,408 + Pf6N2E2_2552 0.62 -2.5 3,013,408 + Pf6N2E2_2552 0.62 -3.0 3,013,409 - Pf6N2E2_2552 0.62 -0.7 3,013,541 - Pf6N2E2_2552 0.73 -0.9 3,013,541 - Pf6N2E2_2552 0.73 -1.2 3,013,741 + Pf6N2E2_2552 0.89 -2.7 3,013,742 - Pf6N2E2_2552 0.89 -1.6 3,014,363 - -1.9
Or see this region's nucleotide sequence