Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2518 and Pf6N2E2_2519 are separated by 28 nucleotides Pf6N2E2_2519 and Pf6N2E2_2520 are separated by 13 nucleotides
Pf6N2E2_2518 - Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), at 2,970,891 to 2,973,098
_2518
Pf6N2E2_2519 - Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57), at 2,973,127 to 2,974,239
_2519
Pf6N2E2_2520 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 2,974,253 to 2,975,347
_2520
Position (kb)
2973
2974
2975 Strain fitness (log2 ratio)
-1
0
1 at 2972.225 kb on - strand, within Pf6N2E2_2518 at 2972.225 kb on - strand, within Pf6N2E2_2518 at 2972.355 kb on - strand, within Pf6N2E2_2518 at 2972.355 kb on - strand, within Pf6N2E2_2518 at 2973.099 kb on - strand at 2973.099 kb on - strand at 2973.191 kb on - strand at 2973.191 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.226 kb on - strand at 2973.226 kb on - strand at 2973.546 kb on - strand, within Pf6N2E2_2519
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 2,972,225 - Pf6N2E2_2518 0.60 -0.9 2,972,225 - Pf6N2E2_2518 0.60 -0.7 2,972,355 - Pf6N2E2_2518 0.66 -0.9 2,972,355 - Pf6N2E2_2518 0.66 +0.3 2,973,099 - -0.3 2,973,099 - -0.4 2,973,191 - +0.0 2,973,191 - +0.5 2,973,222 - +0.1 2,973,222 - -1.7 2,973,222 - +0.2 2,973,222 - +0.3 2,973,222 - -0.7 2,973,226 - +1.7 2,973,226 - +0.1 2,973,546 - Pf6N2E2_2519 0.38 +0.3
Or see this region's nucleotide sequence