Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1560 and Pf6N2E2_1561 are separated by 113 nucleotides Pf6N2E2_1561 and Pf6N2E2_1562 are separated by 154 nucleotides
Pf6N2E2_1560 - Glycine cleavage system transcriptional activator GcvA, at 1,821,858 to 1,822,763
_1560
Pf6N2E2_1561 - Glutathione S-transferase (EC 2.5.1.18), at 1,822,877 to 1,823,476
_1561
Pf6N2E2_1562 - Threonine dehydratase (EC 4.3.1.19), at 1,823,631 to 1,824,587
_1562
Position (kb)
1822
1823
1824 Strain fitness (log2 ratio)
-2
-1
0
1 at 1821.903 kb on - strand at 1821.903 kb on - strand at 1822.028 kb on - strand, within Pf6N2E2_1560 at 1822.373 kb on + strand, within Pf6N2E2_1560 at 1822.373 kb on + strand, within Pf6N2E2_1560 at 1822.558 kb on + strand, within Pf6N2E2_1560 at 1822.558 kb on + strand, within Pf6N2E2_1560 at 1822.703 kb on + strand at 1822.730 kb on + strand at 1822.795 kb on - strand at 1822.799 kb on + strand at 1822.799 kb on + strand at 1822.886 kb on + strand at 1822.887 kb on - strand at 1822.887 kb on - strand at 1823.027 kb on + strand, within Pf6N2E2_1561 at 1823.027 kb on + strand, within Pf6N2E2_1561 at 1823.027 kb on + strand, within Pf6N2E2_1561 at 1823.090 kb on + strand, within Pf6N2E2_1561 at 1823.179 kb on + strand, within Pf6N2E2_1561 at 1823.180 kb on - strand, within Pf6N2E2_1561 at 1823.225 kb on - strand, within Pf6N2E2_1561 at 1823.279 kb on + strand, within Pf6N2E2_1561 at 1823.279 kb on + strand, within Pf6N2E2_1561 at 1823.280 kb on - strand, within Pf6N2E2_1561 at 1823.342 kb on + strand, within Pf6N2E2_1561 at 1823.342 kb on + strand, within Pf6N2E2_1561 at 1823.342 kb on + strand, within Pf6N2E2_1561 at 1823.342 kb on + strand, within Pf6N2E2_1561 at 1823.343 kb on - strand, within Pf6N2E2_1561 at 1823.343 kb on - strand, within Pf6N2E2_1561 at 1823.343 kb on - strand, within Pf6N2E2_1561 at 1823.343 kb on - strand, within Pf6N2E2_1561 at 1823.475 kb on - strand at 1823.650 kb on + strand at 1823.651 kb on - strand at 1823.651 kb on - strand at 1823.666 kb on + strand at 1823.782 kb on + strand, within Pf6N2E2_1562 at 1823.782 kb on + strand, within Pf6N2E2_1562 at 1823.782 kb on + strand, within Pf6N2E2_1562 at 1823.783 kb on - strand, within Pf6N2E2_1562 at 1823.783 kb on - strand, within Pf6N2E2_1562 at 1823.785 kb on + strand, within Pf6N2E2_1562 at 1823.786 kb on - strand, within Pf6N2E2_1562 at 1823.826 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.907 kb on - strand, within Pf6N2E2_1562 at 1823.907 kb on - strand, within Pf6N2E2_1562 at 1824.064 kb on + strand, within Pf6N2E2_1562 at 1824.064 kb on + strand, within Pf6N2E2_1562 at 1824.065 kb on - strand, within Pf6N2E2_1562 at 1824.065 kb on - strand, within Pf6N2E2_1562 at 1824.110 kb on - strand, within Pf6N2E2_1562 at 1824.276 kb on - strand, within Pf6N2E2_1562
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 1,821,903 - -0.1 1,821,903 - +0.7 1,822,028 - Pf6N2E2_1560 0.19 +0.5 1,822,373 + Pf6N2E2_1560 0.57 +0.5 1,822,373 + Pf6N2E2_1560 0.57 -0.2 1,822,558 + Pf6N2E2_1560 0.77 -0.6 1,822,558 + Pf6N2E2_1560 0.77 +1.2 1,822,703 + +0.3 1,822,730 + +1.2 1,822,795 - -0.1 1,822,799 + +0.0 1,822,799 + +0.2 1,822,886 + -0.7 1,822,887 - +0.6 1,822,887 - +0.0 1,823,027 + Pf6N2E2_1561 0.25 +0.0 1,823,027 + Pf6N2E2_1561 0.25 +0.0 1,823,027 + Pf6N2E2_1561 0.25 +0.6 1,823,090 + Pf6N2E2_1561 0.35 +0.5 1,823,179 + Pf6N2E2_1561 0.50 +0.1 1,823,180 - Pf6N2E2_1561 0.51 -0.3 1,823,225 - Pf6N2E2_1561 0.58 -1.5 1,823,279 + Pf6N2E2_1561 0.67 -0.2 1,823,279 + Pf6N2E2_1561 0.67 -1.9 1,823,280 - Pf6N2E2_1561 0.67 -1.7 1,823,342 + Pf6N2E2_1561 0.78 -0.5 1,823,342 + Pf6N2E2_1561 0.78 +0.7 1,823,342 + Pf6N2E2_1561 0.78 +0.3 1,823,342 + Pf6N2E2_1561 0.78 +0.3 1,823,343 - Pf6N2E2_1561 0.78 -0.7 1,823,343 - Pf6N2E2_1561 0.78 -0.9 1,823,343 - Pf6N2E2_1561 0.78 +0.2 1,823,343 - Pf6N2E2_1561 0.78 -0.8 1,823,475 - +0.6 1,823,650 + -0.0 1,823,651 - +0.5 1,823,651 - -2.4 1,823,666 + +1.0 1,823,782 + Pf6N2E2_1562 0.16 +0.9 1,823,782 + Pf6N2E2_1562 0.16 +0.9 1,823,782 + Pf6N2E2_1562 0.16 -1.1 1,823,783 - Pf6N2E2_1562 0.16 -0.3 1,823,783 - Pf6N2E2_1562 0.16 -0.2 1,823,785 + Pf6N2E2_1562 0.16 -1.7 1,823,786 - Pf6N2E2_1562 0.16 -0.1 1,823,826 + Pf6N2E2_1562 0.20 +0.5 1,823,851 + Pf6N2E2_1562 0.23 +0.0 1,823,851 + Pf6N2E2_1562 0.23 -0.7 1,823,851 + Pf6N2E2_1562 0.23 -1.1 1,823,851 + Pf6N2E2_1562 0.23 +0.5 1,823,851 + Pf6N2E2_1562 0.23 +0.3 1,823,851 + Pf6N2E2_1562 0.23 -0.1 1,823,852 - Pf6N2E2_1562 0.23 -2.3 1,823,852 - Pf6N2E2_1562 0.23 +0.2 1,823,852 - Pf6N2E2_1562 0.23 +0.5 1,823,852 - Pf6N2E2_1562 0.23 -0.7 1,823,852 - Pf6N2E2_1562 0.23 +0.9 1,823,907 - Pf6N2E2_1562 0.29 +0.4 1,823,907 - Pf6N2E2_1562 0.29 +0.4 1,824,064 + Pf6N2E2_1562 0.45 +1.4 1,824,064 + Pf6N2E2_1562 0.45 +0.0 1,824,065 - Pf6N2E2_1562 0.45 +1.1 1,824,065 - Pf6N2E2_1562 0.45 +0.9 1,824,110 - Pf6N2E2_1562 0.50 +0.4 1,824,276 - Pf6N2E2_1562 0.67 +0.2
Or see this region's nucleotide sequence