Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1190 and Pf6N2E2_1191 are separated by 147 nucleotides Pf6N2E2_1191 and Pf6N2E2_1192 are separated by 630 nucleotides Pf6N2E2_1192 and Pf6N2E2_1193 are separated by 135 nucleotides
Pf6N2E2_1190 - Glutamine-dependent 2-keto-4-methylthiobutyrate transaminase, at 1,393,302 to 1,394,303
_1190
Pf6N2E2_1191 - Thiaminase II (EC 3.5.99.2), at 1,394,451 to 1,395,161
_1191
Pf6N2E2_1192 - Transcriptional regulator, at 1,395,792 to 1,396,019
_1192
Pf6N2E2_1193 - Outer membrane porin, OprD family, at 1,396,155 to 1,396,385
_1193
Position (kb)
1394
1395
1396 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1393.468 kb on - strand, within Pf6N2E2_1190 at 1393.468 kb on - strand, within Pf6N2E2_1190 at 1393.486 kb on + strand, within Pf6N2E2_1190 at 1393.501 kb on + strand, within Pf6N2E2_1190 at 1393.501 kb on + strand, within Pf6N2E2_1190 at 1393.502 kb on - strand, within Pf6N2E2_1190 at 1393.502 kb on - strand, within Pf6N2E2_1190 at 1393.579 kb on + strand, within Pf6N2E2_1190 at 1393.579 kb on + strand, within Pf6N2E2_1190 at 1393.647 kb on + strand, within Pf6N2E2_1190 at 1393.666 kb on + strand, within Pf6N2E2_1190 at 1393.667 kb on - strand, within Pf6N2E2_1190 at 1393.667 kb on - strand, within Pf6N2E2_1190 at 1393.690 kb on + strand, within Pf6N2E2_1190 at 1393.691 kb on - strand, within Pf6N2E2_1190 at 1393.691 kb on - strand, within Pf6N2E2_1190 at 1393.828 kb on + strand, within Pf6N2E2_1190 at 1393.835 kb on - strand, within Pf6N2E2_1190 at 1393.844 kb on - strand, within Pf6N2E2_1190 at 1393.959 kb on + strand, within Pf6N2E2_1190 at 1393.960 kb on - strand, within Pf6N2E2_1190 at 1394.077 kb on + strand, within Pf6N2E2_1190 at 1394.080 kb on + strand, within Pf6N2E2_1190 at 1394.081 kb on - strand, within Pf6N2E2_1190 at 1394.086 kb on + strand, within Pf6N2E2_1190 at 1394.137 kb on + strand, within Pf6N2E2_1190 at 1394.150 kb on - strand, within Pf6N2E2_1190 at 1394.150 kb on - strand, within Pf6N2E2_1190 at 1394.368 kb on + strand at 1394.368 kb on + strand at 1394.369 kb on - strand at 1394.369 kb on - strand at 1394.441 kb on + strand at 1394.441 kb on + strand at 1394.508 kb on + strand at 1394.531 kb on + strand, within Pf6N2E2_1191 at 1394.532 kb on - strand, within Pf6N2E2_1191 at 1394.532 kb on - strand, within Pf6N2E2_1191 at 1394.620 kb on + strand, within Pf6N2E2_1191 at 1394.621 kb on - strand, within Pf6N2E2_1191 at 1394.626 kb on + strand, within Pf6N2E2_1191 at 1394.626 kb on + strand, within Pf6N2E2_1191 at 1394.627 kb on - strand, within Pf6N2E2_1191 at 1394.627 kb on - strand, within Pf6N2E2_1191 at 1394.656 kb on + strand, within Pf6N2E2_1191 at 1394.657 kb on - strand, within Pf6N2E2_1191 at 1394.674 kb on + strand, within Pf6N2E2_1191 at 1394.716 kb on + strand, within Pf6N2E2_1191 at 1394.716 kb on + strand, within Pf6N2E2_1191 at 1394.717 kb on - strand, within Pf6N2E2_1191 at 1394.717 kb on - strand, within Pf6N2E2_1191 at 1394.717 kb on - strand, within Pf6N2E2_1191 at 1394.717 kb on - strand, within Pf6N2E2_1191 at 1394.717 kb on - strand, within Pf6N2E2_1191 at 1394.799 kb on - strand, within Pf6N2E2_1191 at 1394.799 kb on - strand, within Pf6N2E2_1191 at 1394.800 kb on + strand, within Pf6N2E2_1191 at 1394.800 kb on + strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.847 kb on + strand, within Pf6N2E2_1191 at 1394.848 kb on - strand, within Pf6N2E2_1191 at 1394.848 kb on - strand, within Pf6N2E2_1191 at 1394.982 kb on - strand, within Pf6N2E2_1191 at 1394.982 kb on - strand, within Pf6N2E2_1191 at 1394.989 kb on + strand, within Pf6N2E2_1191 at 1394.990 kb on - strand, within Pf6N2E2_1191 at 1394.990 kb on - strand, within Pf6N2E2_1191 at 1394.998 kb on + strand, within Pf6N2E2_1191 at 1395.056 kb on + strand, within Pf6N2E2_1191 at 1395.057 kb on - strand, within Pf6N2E2_1191 at 1395.057 kb on - strand at 1395.058 kb on + strand, within Pf6N2E2_1191 at 1395.059 kb on - strand, within Pf6N2E2_1191 at 1395.059 kb on - strand, within Pf6N2E2_1191 at 1395.133 kb on + strand at 1395.161 kb on + strand at 1395.161 kb on + strand at 1395.161 kb on + strand at 1395.162 kb on - strand at 1395.162 kb on - strand at 1395.162 kb on - strand at 1395.175 kb on + strand at 1395.176 kb on - strand at 1395.185 kb on + strand at 1395.186 kb on - strand at 1395.186 kb on - strand at 1395.186 kb on - strand at 1395.837 kb on + strand, within Pf6N2E2_1192 at 1395.837 kb on + strand, within Pf6N2E2_1192 at 1395.838 kb on - strand, within Pf6N2E2_1192 at 1395.838 kb on - strand, within Pf6N2E2_1192 at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.906 kb on + strand at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.993 kb on + strand, within Pf6N2E2_1192 at 1395.993 kb on + strand, within Pf6N2E2_1192 at 1396.000 kb on + strand at 1396.000 kb on + strand at 1396.104 kb on + strand at 1396.104 kb on + strand at 1396.105 kb on - strand at 1396.105 kb on - strand at 1396.105 kb on - strand at 1396.111 kb on - strand at 1396.158 kb on + strand at 1396.158 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 1,393,468 - Pf6N2E2_1190 0.17 -1.5 1,393,468 - Pf6N2E2_1190 0.17 -4.1 1,393,486 + Pf6N2E2_1190 0.18 -3.1 1,393,501 + Pf6N2E2_1190 0.20 -3.8 1,393,501 + Pf6N2E2_1190 0.20 -3.5 1,393,502 - Pf6N2E2_1190 0.20 -3.2 1,393,502 - Pf6N2E2_1190 0.20 -2.2 1,393,579 + Pf6N2E2_1190 0.28 -2.9 1,393,579 + Pf6N2E2_1190 0.28 -3.7 1,393,647 + Pf6N2E2_1190 0.34 -2.8 1,393,666 + Pf6N2E2_1190 0.36 -3.2 1,393,667 - Pf6N2E2_1190 0.36 -3.6 1,393,667 - Pf6N2E2_1190 0.36 -4.9 1,393,690 + Pf6N2E2_1190 0.39 -4.4 1,393,691 - Pf6N2E2_1190 0.39 -3.7 1,393,691 - Pf6N2E2_1190 0.39 -2.5 1,393,828 + Pf6N2E2_1190 0.52 -3.8 1,393,835 - Pf6N2E2_1190 0.53 -4.5 1,393,844 - Pf6N2E2_1190 0.54 -3.2 1,393,959 + Pf6N2E2_1190 0.66 -2.2 1,393,960 - Pf6N2E2_1190 0.66 -3.5 1,394,077 + Pf6N2E2_1190 0.77 -2.4 1,394,080 + Pf6N2E2_1190 0.78 -4.4 1,394,081 - Pf6N2E2_1190 0.78 -2.8 1,394,086 + Pf6N2E2_1190 0.78 -4.7 1,394,137 + Pf6N2E2_1190 0.83 -1.2 1,394,150 - Pf6N2E2_1190 0.85 -3.4 1,394,150 - Pf6N2E2_1190 0.85 -3.8 1,394,368 + -3.4 1,394,368 + -3.2 1,394,369 - -3.1 1,394,369 - -4.4 1,394,441 + -1.2 1,394,441 + -3.5 1,394,508 + -3.2 1,394,531 + Pf6N2E2_1191 0.11 -4.1 1,394,532 - Pf6N2E2_1191 0.11 -4.8 1,394,532 - Pf6N2E2_1191 0.11 -2.8 1,394,620 + Pf6N2E2_1191 0.24 -4.4 1,394,621 - Pf6N2E2_1191 0.24 -4.3 1,394,626 + Pf6N2E2_1191 0.25 -3.5 1,394,626 + Pf6N2E2_1191 0.25 -3.4 1,394,627 - Pf6N2E2_1191 0.25 -0.9 1,394,627 - Pf6N2E2_1191 0.25 -2.4 1,394,656 + Pf6N2E2_1191 0.29 -3.4 1,394,657 - Pf6N2E2_1191 0.29 -3.1 1,394,674 + Pf6N2E2_1191 0.31 -0.9 1,394,716 + Pf6N2E2_1191 0.37 -2.8 1,394,716 + Pf6N2E2_1191 0.37 -3.7 1,394,717 - Pf6N2E2_1191 0.37 -2.5 1,394,717 - Pf6N2E2_1191 0.37 -4.6 1,394,717 - Pf6N2E2_1191 0.37 -3.2 1,394,717 - Pf6N2E2_1191 0.37 -3.0 1,394,717 - Pf6N2E2_1191 0.37 -3.2 1,394,799 - Pf6N2E2_1191 0.49 -4.4 1,394,799 - Pf6N2E2_1191 0.49 -3.4 1,394,800 + Pf6N2E2_1191 0.49 -3.2 1,394,800 + Pf6N2E2_1191 0.49 -2.5 1,394,801 - Pf6N2E2_1191 0.49 -2.8 1,394,801 - Pf6N2E2_1191 0.49 -4.2 1,394,801 - Pf6N2E2_1191 0.49 -0.3 1,394,801 - Pf6N2E2_1191 0.49 -3.5 1,394,801 - Pf6N2E2_1191 0.49 -3.4 1,394,801 - Pf6N2E2_1191 0.49 -2.2 1,394,801 - Pf6N2E2_1191 0.49 -2.5 1,394,801 - Pf6N2E2_1191 0.49 -3.0 1,394,801 - Pf6N2E2_1191 0.49 -4.2 1,394,847 + Pf6N2E2_1191 0.56 -3.4 1,394,848 - Pf6N2E2_1191 0.56 -3.4 1,394,848 - Pf6N2E2_1191 0.56 -4.1 1,394,982 - Pf6N2E2_1191 0.75 -3.1 1,394,982 - Pf6N2E2_1191 0.75 -3.9 1,394,989 + Pf6N2E2_1191 0.76 -3.6 1,394,990 - Pf6N2E2_1191 0.76 -4.7 1,394,990 - Pf6N2E2_1191 0.76 -2.8 1,394,998 + Pf6N2E2_1191 0.77 -4.5 1,395,056 + Pf6N2E2_1191 0.85 -4.0 1,395,057 - Pf6N2E2_1191 0.85 -4.3 1,395,057 - -2.5 1,395,058 + Pf6N2E2_1191 0.85 -4.0 1,395,059 - Pf6N2E2_1191 0.86 -3.3 1,395,059 - Pf6N2E2_1191 0.86 -3.4 1,395,133 + -2.0 1,395,161 + -3.7 1,395,161 + -3.2 1,395,161 + -2.9 1,395,162 - -3.2 1,395,162 - -2.2 1,395,162 - -2.8 1,395,175 + -3.0 1,395,176 - -1.2 1,395,185 + -1.5 1,395,186 - -4.7 1,395,186 - -3.0 1,395,186 - -2.8 1,395,837 + Pf6N2E2_1192 0.20 +2.5 1,395,837 + Pf6N2E2_1192 0.20 -0.5 1,395,838 - Pf6N2E2_1192 0.20 +1.2 1,395,838 - Pf6N2E2_1192 0.20 +0.1 1,395,906 + Pf6N2E2_1192 0.50 +0.8 1,395,906 + Pf6N2E2_1192 0.50 -1.4 1,395,906 + +1.0 1,395,906 + Pf6N2E2_1192 0.50 -0.3 1,395,906 + Pf6N2E2_1192 0.50 -0.3 1,395,907 - Pf6N2E2_1192 0.50 +0.2 1,395,907 - Pf6N2E2_1192 0.50 +1.3 1,395,907 - Pf6N2E2_1192 0.50 +0.3 1,395,907 - Pf6N2E2_1192 0.50 +0.5 1,395,993 + Pf6N2E2_1192 0.88 -0.4 1,395,993 + Pf6N2E2_1192 0.88 +0.4 1,396,000 + +1.5 1,396,000 + +0.7 1,396,104 + -1.3 1,396,104 + +1.5 1,396,105 - +0.4 1,396,105 - -0.0 1,396,105 - +0.4 1,396,111 - +0.2 1,396,158 + +0.7 1,396,158 + +1.0
Or see this region's nucleotide sequence