Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_876 and Pf6N2E2_877 are separated by 19 nucleotides Pf6N2E2_877 and Pf6N2E2_878 are separated by 76 nucleotides Pf6N2E2_878 and Pf6N2E2_879 are separated by 238 nucleotides Pf6N2E2_879 and Pf6N2E2_880 are separated by 106 nucleotides
Pf6N2E2_876 - Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84), at 1,025,643 to 1,026,296
_876
Pf6N2E2_877 - Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84), at 1,026,316 to 1,026,948
_877
Pf6N2E2_878 - Amidase clustered with urea ABC transporter and nitrile hydratase functions, at 1,027,025 to 1,028,539
_878
Pf6N2E2_879 - Cyanoglobin; Hemoglobin-like protein HbN, at 1,028,778 to 1,029,302
_879
Pf6N2E2_880 - QbdB, at 1,029,409 to 1,030,275
_880
Position (kb)
1027
1028
1029 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1026.092 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.109 kb on + strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.269 kb on + strand at 1026.269 kb on + strand at 1026.270 kb on - strand at 1026.277 kb on - strand at 1026.286 kb on - strand at 1026.434 kb on - strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.473 kb on - strand, within Pf6N2E2_877 at 1026.493 kb on - strand, within Pf6N2E2_877 at 1026.493 kb on - strand, within Pf6N2E2_877 at 1026.563 kb on + strand, within Pf6N2E2_877 at 1026.563 kb on + strand, within Pf6N2E2_877 at 1026.737 kb on + strand, within Pf6N2E2_877 at 1026.738 kb on - strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.940 kb on - strand at 1026.953 kb on + strand at 1026.953 kb on + strand at 1026.954 kb on - strand at 1026.971 kb on + strand at 1026.987 kb on + strand at 1026.987 kb on + strand at 1026.988 kb on - strand at 1026.988 kb on - strand at 1026.988 kb on - strand at 1027.026 kb on + strand at 1027.027 kb on - strand at 1027.092 kb on + strand at 1027.171 kb on + strand at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.297 kb on - strand, within Pf6N2E2_878 at 1027.297 kb on - strand, within Pf6N2E2_878 at 1027.297 kb on - strand, within Pf6N2E2_878 at 1027.297 kb on - strand, within Pf6N2E2_878 at 1027.318 kb on - strand, within Pf6N2E2_878 at 1027.360 kb on - strand, within Pf6N2E2_878 at 1027.374 kb on + strand, within Pf6N2E2_878 at 1027.375 kb on - strand, within Pf6N2E2_878 at 1027.375 kb on - strand, within Pf6N2E2_878 at 1027.375 kb on - strand, within Pf6N2E2_878 at 1027.482 kb on + strand, within Pf6N2E2_878 at 1027.483 kb on - strand, within Pf6N2E2_878 at 1027.746 kb on + strand, within Pf6N2E2_878 at 1027.746 kb on + strand, within Pf6N2E2_878 at 1027.746 kb on + strand, within Pf6N2E2_878 at 1027.890 kb on + strand, within Pf6N2E2_878 at 1027.890 kb on + strand, within Pf6N2E2_878 at 1027.890 kb on + strand, within Pf6N2E2_878 at 1027.890 kb on + strand, within Pf6N2E2_878 at 1027.891 kb on - strand, within Pf6N2E2_878 at 1027.891 kb on - strand, within Pf6N2E2_878 at 1027.898 kb on + strand, within Pf6N2E2_878 at 1027.899 kb on - strand, within Pf6N2E2_878 at 1027.909 kb on + strand, within Pf6N2E2_878 at 1027.909 kb on + strand, within Pf6N2E2_878 at 1027.909 kb on + strand, within Pf6N2E2_878 at 1027.910 kb on - strand, within Pf6N2E2_878 at 1027.910 kb on - strand, within Pf6N2E2_878 at 1027.910 kb on - strand, within Pf6N2E2_878 at 1027.910 kb on - strand, within Pf6N2E2_878 at 1027.923 kb on + strand, within Pf6N2E2_878 at 1027.924 kb on - strand, within Pf6N2E2_878 at 1027.924 kb on - strand, within Pf6N2E2_878 at 1027.924 kb on - strand, within Pf6N2E2_878 at 1027.924 kb on - strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.044 kb on - strand, within Pf6N2E2_878 at 1028.086 kb on + strand, within Pf6N2E2_878 at 1028.087 kb on - strand, within Pf6N2E2_878 at 1028.087 kb on - strand, within Pf6N2E2_878 at 1028.109 kb on + strand, within Pf6N2E2_878 at 1028.109 kb on + strand, within Pf6N2E2_878 at 1028.189 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.313 kb on + strand, within Pf6N2E2_878 at 1028.314 kb on - strand, within Pf6N2E2_878 at 1028.316 kb on + strand, within Pf6N2E2_878 at 1028.317 kb on - strand, within Pf6N2E2_878 at 1028.418 kb on + strand at 1028.452 kb on - strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.481 kb on - strand at 1028.481 kb on - strand at 1028.481 kb on - strand at 1028.481 kb on - strand at 1028.654 kb on - strand at 1028.661 kb on + strand at 1028.661 kb on + strand at 1028.661 kb on + strand at 1028.662 kb on - strand at 1028.662 kb on - strand at 1028.662 kb on - strand at 1028.759 kb on + strand at 1028.760 kb on - strand at 1028.760 kb on - strand at 1028.765 kb on + strand at 1028.765 kb on + strand at 1028.765 kb on + strand at 1028.766 kb on - strand at 1028.766 kb on - strand at 1028.992 kb on + strand, within Pf6N2E2_879 at 1028.993 kb on - strand, within Pf6N2E2_879 at 1029.082 kb on + strand, within Pf6N2E2_879 at 1029.083 kb on - strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.210 kb on + strand, within Pf6N2E2_879 at 1029.229 kb on + strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.242 kb on - strand, within Pf6N2E2_879 at 1029.258 kb on - strand at 1029.259 kb on + strand at 1029.259 kb on + strand at 1029.260 kb on - strand at 1029.260 kb on - strand at 1029.260 kb on - strand at 1029.260 kb on - strand at 1029.379 kb on - strand at 1029.395 kb on - strand at 1029.411 kb on - strand at 1029.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,026,092 - Pf6N2E2_876 0.69 -1.2 1,026,095 - Pf6N2E2_876 0.69 -1.4 1,026,095 - Pf6N2E2_876 0.69 +0.0 1,026,109 + Pf6N2E2_876 0.71 +0.4 1,026,110 - Pf6N2E2_876 0.71 -0.6 1,026,110 - Pf6N2E2_876 0.71 -0.8 1,026,110 - Pf6N2E2_876 0.71 +0.4 1,026,269 + +0.1 1,026,269 + -1.0 1,026,270 - -0.5 1,026,277 - -1.2 1,026,286 - +1.3 1,026,434 - Pf6N2E2_877 0.19 +0.3 1,026,472 + Pf6N2E2_877 0.25 +2.3 1,026,472 + Pf6N2E2_877 0.25 +0.9 1,026,472 + Pf6N2E2_877 0.25 +0.6 1,026,472 + Pf6N2E2_877 0.25 -0.3 1,026,473 - Pf6N2E2_877 0.25 -0.4 1,026,493 - Pf6N2E2_877 0.28 +0.0 1,026,493 - Pf6N2E2_877 0.28 -0.1 1,026,563 + Pf6N2E2_877 0.39 +0.4 1,026,563 + Pf6N2E2_877 0.39 -0.4 1,026,737 + Pf6N2E2_877 0.67 -0.3 1,026,738 - Pf6N2E2_877 0.67 -0.9 1,026,803 + Pf6N2E2_877 0.77 +0.7 1,026,803 + Pf6N2E2_877 0.77 +0.4 1,026,803 + Pf6N2E2_877 0.77 +0.6 1,026,803 + Pf6N2E2_877 0.77 +1.6 1,026,803 + Pf6N2E2_877 0.77 -1.2 1,026,803 + Pf6N2E2_877 0.77 -0.1 1,026,804 - Pf6N2E2_877 0.77 +1.0 1,026,804 - Pf6N2E2_877 0.77 +0.3 1,026,804 - Pf6N2E2_877 0.77 -1.5 1,026,804 - Pf6N2E2_877 0.77 +0.6 1,026,804 - Pf6N2E2_877 0.77 +0.8 1,026,804 - Pf6N2E2_877 0.77 +0.2 1,026,940 - +0.2 1,026,953 + -0.7 1,026,953 + +1.9 1,026,954 - -0.2 1,026,971 + -1.1 1,026,987 + +1.3 1,026,987 + -0.5 1,026,988 - +0.4 1,026,988 - +0.2 1,026,988 - -0.9 1,027,026 + -0.2 1,027,027 - +1.0 1,027,092 + -0.1 1,027,171 + +1.0 1,027,296 + Pf6N2E2_878 0.18 +0.5 1,027,296 + Pf6N2E2_878 0.18 -0.6 1,027,296 + Pf6N2E2_878 0.18 +0.9 1,027,297 - Pf6N2E2_878 0.18 +0.0 1,027,297 - Pf6N2E2_878 0.18 -0.3 1,027,297 - Pf6N2E2_878 0.18 +0.3 1,027,297 - Pf6N2E2_878 0.18 +1.0 1,027,318 - Pf6N2E2_878 0.19 +0.6 1,027,360 - Pf6N2E2_878 0.22 -1.0 1,027,374 + Pf6N2E2_878 0.23 +0.6 1,027,375 - Pf6N2E2_878 0.23 +1.2 1,027,375 - Pf6N2E2_878 0.23 -0.7 1,027,375 - Pf6N2E2_878 0.23 +0.1 1,027,482 + Pf6N2E2_878 0.30 -0.1 1,027,483 - Pf6N2E2_878 0.30 -0.1 1,027,746 + Pf6N2E2_878 0.48 -2.2 1,027,746 + Pf6N2E2_878 0.48 -2.7 1,027,746 + Pf6N2E2_878 0.48 -1.0 1,027,890 + Pf6N2E2_878 0.57 +0.5 1,027,890 + Pf6N2E2_878 0.57 +1.0 1,027,890 + Pf6N2E2_878 0.57 -3.0 1,027,890 + Pf6N2E2_878 0.57 +1.6 1,027,891 - Pf6N2E2_878 0.57 +0.6 1,027,891 - Pf6N2E2_878 0.57 +1.1 1,027,898 + Pf6N2E2_878 0.58 +1.3 1,027,899 - Pf6N2E2_878 0.58 +0.2 1,027,909 + Pf6N2E2_878 0.58 +0.6 1,027,909 + Pf6N2E2_878 0.58 -0.9 1,027,909 + Pf6N2E2_878 0.58 +0.4 1,027,910 - Pf6N2E2_878 0.58 -0.7 1,027,910 - Pf6N2E2_878 0.58 +0.1 1,027,910 - Pf6N2E2_878 0.58 -0.1 1,027,910 - Pf6N2E2_878 0.58 +0.8 1,027,923 + Pf6N2E2_878 0.59 +0.1 1,027,924 - Pf6N2E2_878 0.59 +0.9 1,027,924 - Pf6N2E2_878 0.59 +1.5 1,027,924 - Pf6N2E2_878 0.59 +0.1 1,027,924 - Pf6N2E2_878 0.59 -0.2 1,028,041 + Pf6N2E2_878 0.67 -0.8 1,028,041 + Pf6N2E2_878 0.67 +0.0 1,028,041 + Pf6N2E2_878 0.67 -0.3 1,028,041 + Pf6N2E2_878 0.67 -0.2 1,028,041 + Pf6N2E2_878 0.67 +1.1 1,028,042 - Pf6N2E2_878 0.67 -1.5 1,028,042 - Pf6N2E2_878 0.67 +0.7 1,028,042 - Pf6N2E2_878 0.67 -0.1 1,028,042 - Pf6N2E2_878 0.67 +0.7 1,028,042 - Pf6N2E2_878 0.67 +0.7 1,028,044 - Pf6N2E2_878 0.67 +0.5 1,028,086 + Pf6N2E2_878 0.70 -0.3 1,028,087 - Pf6N2E2_878 0.70 -0.9 1,028,087 - Pf6N2E2_878 0.70 -0.6 1,028,109 + Pf6N2E2_878 0.72 -0.2 1,028,109 + Pf6N2E2_878 0.72 -0.4 1,028,189 + Pf6N2E2_878 0.77 -2.9 1,028,245 + Pf6N2E2_878 0.81 +0.6 1,028,245 + Pf6N2E2_878 0.81 +0.4 1,028,245 + Pf6N2E2_878 0.81 +0.4 1,028,245 + Pf6N2E2_878 0.81 -1.6 1,028,245 + Pf6N2E2_878 0.81 +0.6 1,028,245 + Pf6N2E2_878 0.81 -1.2 1,028,245 + Pf6N2E2_878 0.81 -0.6 1,028,245 + Pf6N2E2_878 0.81 -1.0 1,028,245 + Pf6N2E2_878 0.81 -1.4 1,028,245 + Pf6N2E2_878 0.81 +0.2 1,028,246 - Pf6N2E2_878 0.81 -0.5 1,028,246 - Pf6N2E2_878 0.81 +0.0 1,028,246 - Pf6N2E2_878 0.81 -0.6 1,028,246 - Pf6N2E2_878 0.81 -0.6 1,028,246 - Pf6N2E2_878 0.81 +0.2 1,028,246 - Pf6N2E2_878 0.81 +0.3 1,028,246 - Pf6N2E2_878 0.81 -2.6 1,028,246 - Pf6N2E2_878 0.81 +0.2 1,028,246 - Pf6N2E2_878 0.81 +0.8 1,028,246 - Pf6N2E2_878 0.81 +0.4 1,028,246 - Pf6N2E2_878 0.81 -0.5 1,028,246 - Pf6N2E2_878 0.81 -1.8 1,028,246 - Pf6N2E2_878 0.81 +0.4 1,028,246 - Pf6N2E2_878 0.81 -0.3 1,028,313 + Pf6N2E2_878 0.85 +1.0 1,028,314 - Pf6N2E2_878 0.85 +0.4 1,028,316 + Pf6N2E2_878 0.85 +1.3 1,028,317 - Pf6N2E2_878 0.85 +0.2 1,028,418 + -0.4 1,028,452 - +0.5 1,028,480 + -0.8 1,028,480 + -0.6 1,028,480 + -1.2 1,028,480 + +0.2 1,028,480 + +0.9 1,028,480 + -0.6 1,028,480 + -1.9 1,028,481 - +1.0 1,028,481 - -0.4 1,028,481 - -0.4 1,028,481 - -0.2 1,028,654 - +1.5 1,028,661 + -0.9 1,028,661 + +0.4 1,028,661 + -2.3 1,028,662 - -0.7 1,028,662 - +0.6 1,028,662 - +0.5 1,028,759 + -0.7 1,028,760 - -0.1 1,028,760 - +0.9 1,028,765 + +0.8 1,028,765 + +0.4 1,028,765 + +0.4 1,028,766 - +0.7 1,028,766 - -0.5 1,028,992 + Pf6N2E2_879 0.41 +0.6 1,028,993 - Pf6N2E2_879 0.41 +0.3 1,029,082 + Pf6N2E2_879 0.58 -1.0 1,029,083 - Pf6N2E2_879 0.58 +0.3 1,029,161 + Pf6N2E2_879 0.73 -0.4 1,029,161 + Pf6N2E2_879 0.73 +0.4 1,029,161 + Pf6N2E2_879 0.73 +2.5 1,029,161 + Pf6N2E2_879 0.73 +1.1 1,029,162 - Pf6N2E2_879 0.73 -0.5 1,029,162 - Pf6N2E2_879 0.73 -0.6 1,029,162 - Pf6N2E2_879 0.73 -1.0 1,029,162 - Pf6N2E2_879 0.73 +0.7 1,029,210 + Pf6N2E2_879 0.82 +0.8 1,029,229 + Pf6N2E2_879 0.86 +1.1 1,029,230 - Pf6N2E2_879 0.86 +2.1 1,029,230 - Pf6N2E2_879 0.86 +0.6 1,029,230 - Pf6N2E2_879 0.86 -0.3 1,029,230 - Pf6N2E2_879 0.86 -2.0 1,029,230 - Pf6N2E2_879 0.86 +0.6 1,029,242 - Pf6N2E2_879 0.88 +1.3 1,029,258 - -0.6 1,029,259 + +0.2 1,029,259 + +0.5 1,029,260 - +0.6 1,029,260 - -0.4 1,029,260 - -0.2 1,029,260 - -1.1 1,029,379 - +0.6 1,029,395 - -0.2 1,029,411 - +1.1 1,029,426 - +0.9
Or see this region's nucleotide sequence