Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6058 and Pf6N2E2_6059 are separated by 32 nucleotides Pf6N2E2_6059 and Pf6N2E2_6060 are separated by 280 nucleotides Pf6N2E2_6060 and Pf6N2E2_6061 overlap by 4 nucleotides Pf6N2E2_6061 and Pf6N2E2_6062 are separated by 41 nucleotides
Pf6N2E2_6058 - FIG139976: hypothetical protein, at 6,877,799 to 6,879,304
_6058
Pf6N2E2_6059 - FIG008443: hypothetical protein, at 6,879,337 to 6,879,873
_6059
Pf6N2E2_6060 - Propionate catabolism operon transcriptional regulator of GntR family [predicted], at 6,880,154 to 6,880,864
_6060
Pf6N2E2_6061 - Methylisocitrate lyase (EC 4.1.3.30) (from data) , at 6,880,861 to 6,881,745
_6061
Pf6N2E2_6062 - 2-methylcitrate synthase (EC 2.3.3.5) (from data) , at 6,881,787 to 6,882,914
_6062
Position (kb)
6880
6881 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6879.338 kb on + strand at 6879.338 kb on + strand at 6879.339 kb on - strand at 6879.386 kb on + strand at 6879.386 kb on + strand at 6879.390 kb on + strand at 6879.390 kb on + strand at 6879.390 kb on + strand at 6879.391 kb on - strand, within Pf6N2E2_6059 at 6879.391 kb on - strand, within Pf6N2E2_6059 at 6879.391 kb on - strand, within Pf6N2E2_6059 at 6879.391 kb on - strand, within Pf6N2E2_6059 at 6879.654 kb on - strand, within Pf6N2E2_6059 at 6879.779 kb on + strand, within Pf6N2E2_6059 at 6879.794 kb on + strand, within Pf6N2E2_6059 at 6879.795 kb on - strand, within Pf6N2E2_6059 at 6879.842 kb on + strand at 6879.842 kb on + strand at 6879.873 kb on + strand at 6879.873 kb on + strand at 6879.873 kb on + strand at 6879.873 kb on + strand at 6879.873 kb on + strand at 6879.874 kb on - strand at 6879.920 kb on + strand at 6879.920 kb on + strand at 6879.920 kb on + strand at 6879.950 kb on + strand at 6879.951 kb on - strand at 6879.952 kb on + strand at 6879.983 kb on + strand at 6879.984 kb on - strand at 6880.060 kb on + strand at 6880.229 kb on - strand, within Pf6N2E2_6060 at 6880.301 kb on + strand, within Pf6N2E2_6060 at 6880.428 kb on - strand, within Pf6N2E2_6060 at 6880.565 kb on + strand, within Pf6N2E2_6060 at 6880.565 kb on + strand, within Pf6N2E2_6060 at 6880.655 kb on + strand, within Pf6N2E2_6060 at 6880.673 kb on + strand, within Pf6N2E2_6060 at 6880.673 kb on + strand, within Pf6N2E2_6060 at 6880.987 kb on + strand, within Pf6N2E2_6061 at 6880.987 kb on + strand, within Pf6N2E2_6061 at 6880.988 kb on - strand, within Pf6N2E2_6061 at 6880.988 kb on - strand, within Pf6N2E2_6061 at 6881.036 kb on + strand, within Pf6N2E2_6061 at 6881.037 kb on - strand, within Pf6N2E2_6061 at 6881.037 kb on - strand, within Pf6N2E2_6061 at 6881.037 kb on - strand, within Pf6N2E2_6061 at 6881.200 kb on - strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.519 kb on - strand, within Pf6N2E2_6061 at 6881.611 kb on + strand, within Pf6N2E2_6061 at 6881.612 kb on - strand, within Pf6N2E2_6061 at 6881.612 kb on - strand, within Pf6N2E2_6061 at 6881.695 kb on + strand at 6881.695 kb on + strand at 6881.743 kb on + strand at 6881.743 kb on + strand at 6881.744 kb on - strand at 6881.744 kb on - strand at 6881.744 kb on - strand at 6881.803 kb on + strand at 6881.803 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 6,879,338 + -1.5 6,879,338 + +0.1 6,879,339 - -0.5 6,879,386 + -1.2 6,879,386 + -1.6 6,879,390 + -0.4 6,879,390 + -0.4 6,879,390 + -2.7 6,879,391 - Pf6N2E2_6059 0.10 +0.0 6,879,391 - Pf6N2E2_6059 0.10 +0.0 6,879,391 - Pf6N2E2_6059 0.10 -0.4 6,879,391 - Pf6N2E2_6059 0.10 +0.5 6,879,654 - Pf6N2E2_6059 0.59 +0.2 6,879,779 + Pf6N2E2_6059 0.82 -0.5 6,879,794 + Pf6N2E2_6059 0.85 -1.0 6,879,795 - Pf6N2E2_6059 0.85 +0.6 6,879,842 + -2.2 6,879,842 + +0.2 6,879,873 + -0.9 6,879,873 + +0.1 6,879,873 + -2.3 6,879,873 + -1.2 6,879,873 + -1.1 6,879,874 - +0.3 6,879,920 + -2.5 6,879,920 + -0.4 6,879,920 + -3.3 6,879,950 + -0.7 6,879,951 - -0.5 6,879,952 + -2.4 6,879,983 + +0.5 6,879,984 - -0.1 6,880,060 + -0.3 6,880,229 - Pf6N2E2_6060 0.11 -0.8 6,880,301 + Pf6N2E2_6060 0.21 -2.9 6,880,428 - Pf6N2E2_6060 0.39 -1.1 6,880,565 + Pf6N2E2_6060 0.58 +1.6 6,880,565 + Pf6N2E2_6060 0.58 -0.7 6,880,655 + Pf6N2E2_6060 0.70 -0.6 6,880,673 + Pf6N2E2_6060 0.73 +1.6 6,880,673 + Pf6N2E2_6060 0.73 -1.5 6,880,987 + Pf6N2E2_6061 0.14 -3.0 6,880,987 + Pf6N2E2_6061 0.14 -2.3 6,880,988 - Pf6N2E2_6061 0.14 -2.3 6,880,988 - Pf6N2E2_6061 0.14 -1.4 6,881,036 + Pf6N2E2_6061 0.20 -1.5 6,881,037 - Pf6N2E2_6061 0.20 -0.8 6,881,037 - Pf6N2E2_6061 0.20 -0.7 6,881,037 - Pf6N2E2_6061 0.20 -0.7 6,881,200 - Pf6N2E2_6061 0.38 -1.4 6,881,446 + Pf6N2E2_6061 0.66 -2.1 6,881,446 + Pf6N2E2_6061 0.66 -2.9 6,881,446 + Pf6N2E2_6061 0.66 -1.1 6,881,446 + Pf6N2E2_6061 0.66 -2.1 6,881,446 + Pf6N2E2_6061 0.66 -2.9 6,881,446 + Pf6N2E2_6061 0.66 -0.7 6,881,447 - Pf6N2E2_6061 0.66 -3.0 6,881,447 - Pf6N2E2_6061 0.66 -1.8 6,881,447 - Pf6N2E2_6061 0.66 -1.5 6,881,447 - Pf6N2E2_6061 0.66 -1.9 6,881,447 - Pf6N2E2_6061 0.66 -1.4 6,881,447 - Pf6N2E2_6061 0.66 -2.1 6,881,447 - Pf6N2E2_6061 0.66 -1.5 6,881,519 - Pf6N2E2_6061 0.74 -1.1 6,881,611 + Pf6N2E2_6061 0.85 -3.1 6,881,612 - Pf6N2E2_6061 0.85 -1.4 6,881,612 - Pf6N2E2_6061 0.85 -0.3 6,881,695 + -1.5 6,881,695 + -1.5 6,881,743 + +0.9 6,881,743 + -0.9 6,881,744 - -0.3 6,881,744 - -2.4 6,881,744 - -0.3 6,881,803 + -0.3 6,881,803 + -1.8
Or see this region's nucleotide sequence